Estrogenic and thyroid hormone activity of a series of hydroxy-polychlorinated biphenyls

Shiraishi, Fujio; Okumura, Tameo; Nomachi, Makoto; Serizawa, Shigeko; Nishikawa, J; Edmonds, John S.; Shiraishi, Hiroaki; Morita, Masatoshi

doi:10.1016/s0045-6535(03)00261-3

Cited by 69 publications

(32 citation statements)

References 14 publications

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“…A portion of the total RNA (100 ng quantified by spectrophotometry at 260 nm) was reverse transcribed with 0.5 mg oligo dT 15 primer in a final volume of 25 ml at 378C for 60 min using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega), and the resultant complementary DNA was diluted four times with nuclease-free water. A portion of the diluted complementary DNA solution (2 ml) was amplified by GoTaq 1 DNA polymerase (Promega), together with 0.5 mM each of the specific primer combinations for target mRNAs described in the Supplemental Data (Table S1), in a total volume of 50 ml.…”

Section: Gene Expressionmentioning

confidence: 99%

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The feasibility of using mosquitofish (Gambusia affinis) for detecting endocrine-disrupting chemicals in the freshwater environment

Kamata

Itoh

Nakajima

et al. 2011

Environmental Toxicology and Chemistry

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We evaluated the utility of gene-transcriptional responses in the liver of mosquitofish (Gambusia affinis), a species introduced to many countries and therefore widely available, for detecting endocrine-disrupting activity in water. Exposure to β-naphthoflavone, an aryl hydrocarbon receptor (AhR) agonist, significantly increased the transcript of the cytochrome P4501A gene (cyp1a), peaking at 24 h, in both sexes at concentrations of 10 µg/L or more. 17β-Estradiol (E(2) ) at 500 ng/L increased the number of males showing gene transcription of precursors of yolk protein, vitellogenin (Vtga, Vtgb, and Vtgc), at 24, 48, and 72 h. Exposure for 48 h to bisphenol A (BPA), an estrogen mimic, also increased vtg-positive males at 1 mg/L or more. Leachate from a Japanese stable-type landfill significantly increased vtg-positive males after 48 h exposure, and the in vitro activity of the leachate against the estrogen receptor (ER) was estimated as an E(2) equivalent of 240 ng/L by yeast transfected with the ER. Chemical analysis showed that major contributors to the ER activation were BPA and 4-tert-octylphenol. This leachate and drainage water from a control-type landfill had AhR activities, estimated by yeast with the AhR, but had no significant effect on cyp1a transcription. These results showed that mosquitofish are suitable for detecting in vivo AhR and ER effects, but are insensitive to E(2).

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Section: Gene Expressionmentioning

confidence: 99%

“…The assays were performed as previously reported for screening chemicals [13][14][15]. Briefly, yeast cells were preincubated for 24 h at 30 8C in modified synthetic dextrose medium.…”

Section: Recombinant Yeast Assaymentioning

confidence: 99%

The feasibility of using mosquitofish (Gambusia affinis) for detecting endocrine-disrupting chemicals in the freshwater environment

Kamata

Itoh

Nakajima

et al. 2011

Environmental Toxicology and Chemistry

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“…The details of the assay have been published elsewhere. 33) The estrogenic activity was recorded as the effective concentration (EC) × 10, which is defined as the concentration of the test solution required to produce a chemiluminescent signal 10 times stronger than that of the blank control. The relative activity (RA) was calculated on the basis of the follows equation: RA (%) = (EC × 10 for BPA)/(EC × 10 for test compounds) × 100.…”

Section: Methodsmentioning

confidence: 99%

Estrogenic Activity of Alkyl(thio)phenols and 4,4'-thiodiphenol Formed from Degradation of Commercial Insecticides

Yamada

Terasaki

Makino

2011

JOURNAL OF HEALTH SCIENCE

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We investigated the estrogenic activity of commercial insecticides fenamiphos, fenthion, methiocarb, propaphos, sulprophos, and temephos as well as some phenolic compounds obtained as a result of their degradation. Using a yeast two-hybrid assay, the relative activities of 4-(methylthio)phenol, 3-methyl-4-(methylthio)phenol, 3,5-dimethyl-4-(methylthio)phenol, and 4,4 -thiodiphenol (TDP) were evaluated as 11, 10, 4, and 1000% (10 times) that of bisphenol-A. To reveal the binding abilities of the abovementioned phenolic compounds with respect to human estrogen receptor α (hERα), we carried out ER-ELISA and found that all compounds had significant abilities, particularly, TDP. From the viewpoint of bioisosterism, we discussed the similarity between a vinylene-group, -CH=CH-, and a thioether-group, -S-. We suggest that an alkylthio-group substituted at the "para"-position of a phenol ring plays a key role in the binding abilities of the investigated phenolic compounds.

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“…The concerns with respect to likely carcinogenic influence on mammary tissues with exposure to PCBs derived from the estrogenic activity of these compounds, which have the ability to mimic or interfere with the action of endogenous hormones [15][16][17]. Because of their lipophilic nature, PCBs tend to accumulate in adipose tissues, and therefore could be detectable in human breast tissues and even in milk.…”

Section: Environmental Pcb Exposure and The Etiology Of Breast Cancermentioning

confidence: 99%

“…Breast cancer incidence rates have rapidly increased in the last 5 decades, and the current estimates indicate approximately 1 out of 8 women globally will develop breast cancer over her lifetime. There is increasing evidence from epidemiological and animal studies that the initiation and progression of breast cancer may be considerably attributed to environmental pollution, such as the environmental endocrine-disrupting compounds [15]. The weakly estrogenic substances (e.g., PCBs and DDT) have been etiologically concerned in the development or progression of breast cancer over years.…”

Section: Introductionmentioning

confidence: 99%

The associations between the environmental exposure to polychlorinated biphenyls (PCBs) and breast cancer risk and progression

Liu

Zhang

et al. 2010

Sci. China Chem.

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Polychlorinated biphenyls (PCBs) are chlorinated biphenyl compounds with wide applications in the industry. In spite of a ban on their production in the late 1970s, PCBs, as a group of POPs, are still persistent and widely spread in the environment, posing potential threats to human health. The role of PCBs as etiologic agents for breast cancer has been intensively explored in a variety of in vivo, animal and epidemiologic studies. Initial investigations indicated higher levels of PCBs in mammary tissues or sera corresponded to the occurrence of breast cancer, but later studies showed no positive association between PCB exposure and breast cancer development. More recent data suggested that the CYP1A1 m2 polymorphisms might add increased risk to the etiology of breast cancer in women with environmental exposure to PCBs. PCBs are implicated in advancing breast cancer progression, and our unpublished data reveals that PCBs activate the ROCK signaling to enhance breast cancer metastasis. Therefore, the correlation between PCB exposure and breast cancer risk warrants further careful investigations.Polychlorinated biphenyls (PCBs), environmental exposure, CYP1A1 polymorphisms, breast cancer risk, tumor progression

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Estrogenic and thyroid hormone activity of a series of hydroxy-polychlorinated biphenyls

Cited by 69 publications

References 14 publications

The feasibility of using mosquitofish (Gambusia affinis) for detecting endocrine-disrupting chemicals in the freshwater environment

The feasibility of using mosquitofish (Gambusia affinis) for detecting endocrine-disrupting chemicals in the freshwater environment

Estrogenic Activity of Alkyl(thio)phenols and 4,4'-thiodiphenol Formed from Degradation of Commercial Insecticides

The associations between the environmental exposure to polychlorinated biphenyls (PCBs) and breast cancer risk and progression

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