Ethanol Gluconobacter biosensor designed for flow injection analysis

Valach, M.; Katrlı́k, Jaroslav; Šturdı́k, Ernest; Gemeiner, Peter

doi:10.1016/j.snb.2009.02.017

Cited by 34 publications

(15 citation statements)

References 24 publications

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“…The response of the biosensor for ethanol was linear in the range from 10 μM to 1.5 mM. Ethanol concentrations obtained were in a good correlation with a gas chromatography reference method (Valach et al 2009). Tkac et al prepared a ferricyanide-mediated microbial biosensors by surface modification of a glassy carbon electrode.…”

Section: Ethanolmentioning

confidence: 61%

Application of Enzyme Biosensors in Analysis of Food and Beverages

et al. 2011

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The importance of analyses of different parameters in food products and monitoring of a production process requires quick and reliable analytical methods and devices. For this purpose, biosensors can be a suitable option, whereas most of the current quality control techniques are time consuming, expensive, and unpractical. In this paper, we describe biosensors developed for analysis of different components present in food samples, namely, glucose, fructose, sucrose, lactose, lactic, malic, acetic, ascorbic, citric and amino acids, ethanol, glycerol, and triglyceride. Biosensors showed desirable sensitivity, selectivity, and response time required for various applications. They are often designed to avoid interference from components present in a complex sample to be analyzed.

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Section: Ethanolmentioning

confidence: 61%

Application of Enzyme Biosensors in Analysis of Food and Beverages

et al. 2011

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“…29 Stabilization of the quaternary structure of multimeric enzymes such as GOx and PyOx may require the use of a further crosslinking step to prevent subunit dissociation. 30,31 The glutaraldehyde technique is very versatile to achieve multipoint attachments. 32 In terms of stabilization, the treatment of enzymes that previously adsorbed on dendrimers bearing primary amino groups with glutaraldehyde, in many cases offers very good results, because this technique permits the crosslink between glutaraldehyde molecules bound to the enzyme and glutaraldehyde molecules bound to the dendrimer.…”

Section: Introductionmentioning

confidence: 99%

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

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Akin

Geyik

et al. 2011

Biotechnology Progress

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This article deals with the use of pyranose oxidase (PyOx) and glucose oxidase (GOx) enzymes in amperometric biosensor design and their application in monitoring fermentation processes with the combination of flow injection analysis (FIA). The amperometric studies were carried out at -0.7 V by following the oxygen consumption due to the enzymatic reactions for both batch and FIA modes. Optimization studies (enzyme amounts and pH) and analytical parameters such as linearity, repeatability, effect of interference, storage, and operational stabilities have been studied. Under optimized conditions, for the PyOx-based biosensor, linear graph was obtained from 0.025 to 0.5 mM glucose in phosphate buffer (50 mM) at pH 7.0 with the equation of y = 3.358x + 0.028 and R(2) = 0.998. Linearity was found to be 0.01-1.0 mM in citrate buffer (50 mM and pH 4.0) with the equation of y = 1.539x + 0.181 and R(2) = 0.992 for the GOx biosensor. Finally, these biosensor configurations were further evaluated in a conventional flow injection system. Results from batch experiments provide a guide to design sensitive, stable, and interference-free biosensors for FIA mode. Biosensor stability, dynamic range, and repeatability were also studied in FIA conditions, and the applicability for the determination of glucose in fermentation medium could be successfully demonstrated. The FIA-combined glucose biosensor was used for the offline monitoring of yeast fermentation. The obtained results correlated well with HPLC measurements.

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“… Assessing product stability towards, water activity, solute concentration, pH value, preservative concentration, protective atmosphere composition, chemical reaction and microbial growth (Lowe, 1985).  Biosensors that use whole cells or enzymes have been used for the detection of alcohol (Valach at al., 2009). A.…”

Section: Application Of Bio-sensorsmentioning

confidence: 99%