Ethanol-induced changes in the expression of proteins related to neurotransmission and metabolism in different regions of the rat brain

Zahr, Natalie M.; Bell, Richard L.; Ringham, Heather N.; Sullivan, Edith V.; Witzmann, Frank A.; Pfefferbaum, Adolf

doi:10.1016/j.pbb.2011.03.002

Cited by 11 publications

(9 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ethanol vapor inhalation procedure and the chambers used in this study were previously described (see O'Dell et al, 2004; Roberts et al, 1996, 2000; Rogers et al, 1979; Slawecki et al, 2001; Zahr et al, 2011). Ethanol vapor chambers were calibrated to produce high to moderate BECs between 150–225 mg/dL.…”

Section: Methodsmentioning

confidence: 99%

Peri-Adolescent Ethanol Vapor Exposure Produces Reductions in Hippocampal Volume that are Correlated with Deficits in Prepulse Inhibition of the Startle

Ehlers

Oguz

Budin

et al. 2013

Alcohol Clin Exp Res

View full text Add to dashboard Cite

BACKGROUND Epidemiological studies suggest that excessive alcohol consumption is prevalent among adolescents and may have lasting neurobehavioral consequences. The use of animal models allows for the separation of the effects of adolescent ethanol exposure from genetic background and other environmental insults. In the present study the effects of moderate ethanol vapor exposure, during adolescence, on structural diffusion tensor imaging (DTI) and behavioral measures were evaluated in adulthood. METHODS A total of 53 Wistar rats were received at postnatal day (PD) 21, and were randomly assigned to ethanol vapor (14 hrs on/10 hrs off/day) or air exposure for 35 days from PD 23-PD 58 (average blood ethanol concentration (BEC): 169 mg%). Animals were received in two groups that were subsequently sacrificed at two time points following withdrawal from ethanol vapor: (1) at 72 days of age, 2 weeks following withdrawal or (2) at day 128, 10 weeks following withdrawal. In the second group, behavior in the light/dark box and prepulse inhibition of the startle (PPI) were also evaluated. Fifteen animals in each group were scanned, post mortem, for structural DTI. RESULTS There were no significant differences in body weight between ethanol and control animals. Volumetric data, demonstrated that total brain, hippocampal, corpus callosum but not ventricular volume was significantly larger in the 128 day sacrificed animals as compared to the 72 day animals. The hippocampus was smaller and the ventricles larger at 128 days as compared to 72 days, in the ethanol exposed animals, leading to a significant group × time effect. Ethanol exposed animals sacrificed at 128 days also had diminished PPI and more rears in the light box that were significantly correlated with hippocampal size. CONCLUSIONS These studies demonstrate that DTI volumetric measures of hippocampus are significantly impacted by age and periadolescent ethanol exposure and withdrawal in Wistar rats.

show abstract

Section: Methodsmentioning

confidence: 99%

Peri-Adolescent Ethanol Vapor Exposure Produces Reductions in Hippocampal Volume that are Correlated with Deficits in Prepulse Inhibition of the Startle

Ehlers

Oguz

Budin

et al. 2013

Alcohol Clin Exp Res

View full text Add to dashboard Cite

show abstract

“…56 Briefly, frozen pulverized cortex tissue from protein overload and control rats was solubilized in 8 M urea, 4% CHAPS, 100 mM DTT, 40 mM Tris-Base pH 9.5, 0.4% carrier ampholytes (3)(4)(5)(6)(7)(8)(9)(10). 200 mg of protein was loaded onto IPG strips (11 cm, nonlinear pH 3-10, Bio-Rad, Hercules, CA) using overnight passive rehydration at room temperature.…”

Section: Immunofluorescence Of Rat Kidney Sectionsmentioning

confidence: 99%

“…The resulting 16-bit Tiff images were analyzed using Progenesis SameSpots TM (v3.0, Nonlinear Dynamics, Durham, NC) and its embedded AN-OVA analytic software as previously described. 56 For mass spectrometry, protein spots showing significant changes with protein overload were manually excised and analyzed as previously described. 56,58 The acquired mass spectral data were searched against the International Protein Index rat database using SEQUEST, validated using PeptideProphet and ProteinProphet in the TransProteomic Pipeline 59,60 and only those proteins with greater than 90% confidence were considered identifications.…”

Section: Immunofluorescence Of Rat Kidney Sectionsmentioning

confidence: 99%

Proximal Tubules Have the Capacity to Regulate Uptake of Albumin

Wagner

Campos-Bilderback

Chowdhury

et al. 2016

Journal of the American Society of Nephrology

Self Cite

View full text Add to dashboard Cite

Evidence from multiple studies supports the concept that both glomerular filtration and proximal tubule (PT) reclamation affect urinary albumin excretion rate. To better understand these roles of glomerular filtration and PT uptake, we investigated these processes in two distinct animal models. In a rat model of acute exogenous albumin overload, we quantified glomerular sieving coefficients (GSC) and PT uptake of Texas Red-labeled rat serum albumin using two-photon intravital microscopy. No change in GSC was observed, but a significant decrease in PT albumin uptake was quantified. In a second model, loss of endogenous albumin was induced in rats by podocyte-specific transgenic expression of diphtheria toxin receptor. In these albumin-deficient rats, exposure to diphtheria toxin induced an increase in albumin GSC and albumin filtration, resulting in increased exposure of the PTs to endogenous albumin. In this case, PT albumin reabsorption was markedly increased. Analysis of known albumin receptors and assessment of cortical protein expression in the albumin overload model, conducted to identify potential proteins and pathways affected by acute protein overload, revealed changes in the expression levels of calreticulin, disabled homolog 2, NRF2, angiopoietin-2, and proteins involved in ATP synthesis. Taken together, these results suggest that a regulated PT cell albumin uptake system can respond rapidly to different physiologic conditions to minimize alterations in serum albumin level. 27: 482-494, 201627: 482-494, . doi: 10.1681 While the clinical relevance of proteinuria, and especially albuminuria, has been well documented, the quantitative and mechanistic significance of different components to albumin excretion remains an area of considerable excitement and debate. 1 Recent data from several laboratories utilizing different approaches have delineated a role of proximal tubules (PTs) in regulation of albumin reabsorption and reclamation. 2 Furthermore, albumin transcytosis has been visualized in PT cells 3 and FcRn has been shown to mediate the transcytosis of albumin across PTCs, 4 as it is known to do for many other cell types. 2 Therefore, the present studies were conducted to begin differentiating and quantifying glomerular and PT contributions to albuminuria under physiologic and disease conditions. This understanding is important because before appropriate therapies can be developed to modulate albuminuria, the structural, functional and mechanistic characterization need to be better understood and interrelated. J Am Soc Nephrol

show abstract

“…The ethanol vapor inhalation procedure and the cages used in this study have been previously described (Rogers et al, 1979; Slawecki et al, 2001; Slawecki, 2002; O’Dell et al, 2004; Funk et al, 2006; Gilpin et al, 2008; Ehlers et al, 2011, 2013; Zahr et al, 2011). Ethanol vapor exposure was calibrated to produce high to moderate BECs between 150–225 mg/dL.…”

Section: 0 Experimental Proceduresmentioning

confidence: 99%

Periadolescent ethanol vapor exposure persistently reduces measures of hippocampal neurogenesis that are associated with behavioral outcomes in adulthood

Ehlers¹,

Liu²,

Wills³

et al. 2013

Neuroscience

100

View full text Add to dashboard Cite

Excessive alcohol consumption is prevalent among adolescents and may result in lasting neurobehavioral consequences. The use of animal models to study adolescent alcohol exposure has the advantage of allowing for the control necessary in order to evaluate the effects of ethanol on the brain and separate such effects from genetic background and other environmental insults. In the present study the effects of moderate ethanol vapor exposure, during adolescence, on measures of neurogenesis and behavioral measures were evaluated at two different times following ethanol withdrawal, in adulthood. The two groups of Wistar rats were both exposed to intermittent ethanol vapor (14 hrs on/10 hrs off/day) for 35–36 days from PD 23-PD 58 (average blood ethanol concentration (BEC): 163 mg%). In the first group, after rats were withdrawal from vapor they were subsequently assessed for locomotor activity, conflict behavior in the open field, and behaviors in the forced swim test and then sacrificed at 72 days of age. The second group of rats were withdrawn from vapor and injected for 5 days with Bromo-deoxy-Uridine (BrdU). Over the next 8 weeks they were also assessed for locomotor activity, conflict behavior in the open field, and behaviors in the forced swim test and then sacrificed at 113/114 days of age. All rats were perfused for histochemical analyses. Ethanol vapor exposed rats displayed hypoactivity in tests of locomotion and less anxiety-like and/or more “disinhibitory” behavior in the open field conflict. Quantitative analyses of immunoreactivity revealed a significant reduction in measures of neurogenesis, progenitor proliferation, as indexed by doublecortin (DCX), Ki67, and increased markers of cell death as indexed by cleaved caspase-3, and Fluoro-Jade at 72 days, and decreases in doublecortin (DCX), and increases in cleaved caspase-3 at 114 days in the ethanol vapor exposed rats. Progenitor survival, as assessed by BrdU+, was reduced in the vapor exposed animals that were sacrificed at 114 days. The reduction seen in DCX labeled in cell counts was significantly correlated with hypoactivity at 24 hours after withdrawal as well as less anxiety-like and/or more “disinhibitory” behavior in the open field conflict test at 2 and 8 weeks following termination of vapor exposure. These studies demonstrate that behavioral measures of disinhibitory behavior correlated with decreases in neurogenesis are all significantly and persistently impacted by periadolescent ethanol exposure and withdrawal in Wistar rats.

show abstract

Ethanol-induced changes in the expression of proteins related to neurotransmission and metabolism in different regions of the rat brain

Cited by 11 publications

References 94 publications

Peri-Adolescent Ethanol Vapor Exposure Produces Reductions in Hippocampal Volume that are Correlated with Deficits in Prepulse Inhibition of the Startle

Peri-Adolescent Ethanol Vapor Exposure Produces Reductions in Hippocampal Volume that are Correlated with Deficits in Prepulse Inhibition of the Startle

Proximal Tubules Have the Capacity to Regulate Uptake of Albumin

Periadolescent ethanol vapor exposure persistently reduces measures of hippocampal neurogenesis that are associated with behavioral outcomes in adulthood

Contact Info

Product

Resources

About