Ethanol production by a mixed culture of flocculent strains of Zymomonas mobilis and Saccharomyces sp.

Abate, Carlos Mauricio; Callieri, D. A. S.; Rodríguez, E.; Garro, Oscar Alfredo

doi:10.1007/s002530050732

Cited by 26 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results clearly demonstrated that starch saccharification potential of S. diastaticus was higher than that of R. stolonifer and A. terreus. Similar results have been reported by Abate et al (1996).…”

Section: Fermentation Using a Terreus And Z Mobilissupporting

confidence: 92%

See 1 more Smart Citation

Simultaneous saccharification and fermentation of cassava waste for ethanol production

Pothiraj

Arun

Eyini³

2015

Biofuel Res. J.

View full text Add to dashboard Cite

Please cite this article as: Pothiraj C., Arun A., Eyini M. Simultaneous saccharification and fermentation of cassava waste for ethanol production. The efficiency of enzymatic and microbial saccharification of cassava waste for ethanol production was investigated and the effective parameters were optimized. The mixture of amylase and amyloglucosidase (AMG) resulted in a significantly higher rate of saccharification (79.6%) than the amylase alone (68.7%). Simultaneous saccharification and fermentation (SSF) yielded 6.2 g L -1 ethanol representing 64.5% of the theoretical yield. Saccharification and fermentation using pure and cocultures of fungal isolates including Rhizopus stolonifer, Aspergillus terreus, Saccharomyces diastaticus and Zymomonas mobilis revealed that the co-culture system involving S. diastaticus and Z. mobilis was highly suitable for the bio-conversion of cassava waste into ethanol, resulting in 20.4 g L -1 in 36 h (91.3% of the theoretical yield).

show abstract

Section: Fermentation Using a Terreus And Z Mobilissupporting

confidence: 92%

“…The supernatant obtained by centrifugation of the culture broth at 6,000 ×g was used for reducing sugars (Miller, 1959) and ethanol (Caputi et al, 1968) analyses. The kinetic parameters of ethanol fermentation were calculated according to Abate et al (1996).…”

Section: Methodsmentioning

confidence: 99%

Simultaneous saccharification and fermentation of cassava waste for ethanol production

Pothiraj

Arun

Eyini³

2015

Biofuel Res. J.

View full text Add to dashboard Cite

show abstract

“…The microbial biomass was determined by harvesting cells by centrifugation, drying them at 70°C under vacuum to a constant weight, and expressed as gram dry cell weight (DCW) per liter [27]. The kinetic parameters of ethanol fermentation were determined followed by Abate et al [28] as follows:…”

Section: Estimationsmentioning

confidence: 99%

Sustaining ethanol production from lime pretreated water hyacinth biomass using mono and co-cultures of isolated fungal strains with Pichia stipitis

Pothiraj

Arumugam

Gobinath³

2014

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Background: The high rate of propagation and easy availability of water hyacinth has made it a renewable carbon source for biofuel production. The present study was undertaken to screen the feasibility of using water hyacinth's hemicelluloses as a substrate for alcohol production by microbial fermentation using mono and co-cultures of Trichoderma reesei and Fusarium oxysporum with Pichia stipitis. Results: In separate hydrolysis and fermentation (SHF), the alkali pretreated water hyacinth biomass was saccharified by crude fungal enzymes of T. reesei, F. oxysporum and then fermented by P. stipitis. In simultaneous saccharification and fermentation (SSF), the saccharification and fermentation was carried out simultaneously at optimized conditions using mono and co-cultures of selected fungal strains. Finally, the ethanol production kinetics were analyzed by appropriate methods. The higher crystalline index (66.7%) and the Fourier transform infrared (FTIR) spectra showed that the lime pretreatment possibly increased the availability of cellulose and hemicelluloses for enzymatic conversion. In SSF, the co-culture fermentation using T. reesei and P. stipitis was found to be promising with a higher yield of ethanol (0.411 g g

show abstract

“…For examples, Abate et al (1996) described ethanol production by a co-culture of Z. mobilis and Saccharomyces sp. with higher yields and production rates than with either microorganism in pure culture.…”

Section: Introductionmentioning

confidence: 99%

Engineered microbial consortium for the efficient conversion of biomass to biofuels: A preliminary study

Anieto¹

2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

This work showed ethanol production by a microbial consortium of Clostridium cellulolyticum and a recombinant Zymomonas mobilis (ZM4 pAA1). The ZM4 pAA1 and wild type ZM4 (ZM4 WT) were first tested on RM medium (ATCC 1341) containing 2% cellobiose as the carbon source. Ethanol production from ZM4 pAA1 was three times higher than that observed from the ZM4 WT. Concomitant with ethanol production was the reduction in OD from 2.00 to 1.580. The ZM4 pAA1 was then co-cultured with C. cellulolyticum using cellobiose and microcrystalline cellulose , respectively, as carbon sources. Results indicate that the ZM4 pAA1 with C. cellulolyticum utilized 2.0 g/L cellobiose, producing as much as 0.40 mM of ethanol, whereas only 0.20 mM ethanol was detected for the ZM4 WT co-cultured with C. cellulolyticum under similar conditions. A consortium of the ZM4 pAA1 and C. cellulolyticum using 7.5 g/L microcrystalline cellulose gave a far lower ethanol yi eld than when using cellobiose. In the latter case, ethanol production was detected within 5 days, whereas it took about 10 days for ethanol to be detectable for the ZM4 WT and C. cellulolyticum. Future efforts will concentrate on identifying suitable partners for the ZM4 pAA1, the correct concentration of feedstocks at which synergy will be observed, as well as optimize medium formulations and inoculation techniques.

show abstract

Ethanol production by a mixed culture of flocculent strains of Zymomonas mobilis and Saccharomyces sp.

Cited by 26 publications

References 12 publications

Simultaneous saccharification and fermentation of cassava waste for ethanol production

Simultaneous saccharification and fermentation of cassava waste for ethanol production

Sustaining ethanol production from lime pretreated water hyacinth biomass using mono and co-cultures of isolated fungal strains with Pichia stipitis

Engineered microbial consortium for the efficient conversion of biomass to biofuels: A preliminary study

Contact Info

Product

Resources

About