Ethanol production by a new pentose‐fermenting yeast strain, Scheffersomyces stipitis UFMG‐IMH 43.2, isolated from the Brazilian forest

Ferreira, Adriana Dilon; Mussatto, Solange I.; Cadete, Raquel M.; Rosa, Carlos A.; Silva, Sílvio S.

doi:10.1002/yea.1858

Cited by 50 publications

(43 citation statements)

References 17 publications

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“…Pichia stipitis (Nigam [2001]) and Scheffersomyces stipitis (Liu et al [2010]) are well described within the context of microbial ethanol production, but have not been mentioned previously with regards to lipid production. Furthermore they are able to assimilate hemicellulosic compounds (Ferreira et al [2011]; Nigam [2001]).…”

Section: Discussionmentioning

confidence: 99%

Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis

et al. 2014

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The yeast strains Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis were isolated from soil samples and identified as oleaginous yeast strains beneficial for the establishment of microbial production processes for sustainable lipid production suitable for several industrial applications. When cultured in bioreactors with glucose as the sole carbon source C. podzolicus yielded 31.8% lipid per dry biomass at 20°C, while T. porosum yielded 34.1% at 25°C and P. segobiensis 24.6% at 25°C. These amounts correspond to lipid concentrations of 17.97 g/L, 17.02 g/L and 12.7 g/L and volumetric productivities of 0.09 g/Lh, 0.1 g/Lh and 0.07 g/Lh, respectively. During the culture of C. podzolicus 30 g/l gluconic acid was detected as by-product in the culture broth and 12 g/L gluconic acid in T. porosum culture. The production of gluconic acid was eliminated for both strains when glucose was substituted by xylose as the carbon source. Using xylose lipid yields were 11.1 g/L and 13.9 g/L, corresponding to 26.8% and 33.4% lipid per dry biomass and a volumetric productivity of 0.07 g/Lh and 0.09 g/Lh, for C. podzolicus and T. porosum respectively. The fatty acid profile analysis showed that oleic acid was the main component (39.6 to 59.4%) in all three strains and could be applicable for biodiesel production. Palmitic acid (18.4 to 21.1%) and linolenic acid (7.5 to 18.7%) are valuable for cosmetic applications. P. segobiensis had a considerable amount of palmitoleic acid (16% content) and may be suitable for medical applications.

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Section: Discussionmentioning

confidence: 99%

Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis

et al. 2014

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“…The inoculum was prepared by transferring a loopful of strains from the slant into 125 ml Erlenmeyer flasks containing 50 ml of synthetic medium composed by 30.0 g/l of xylose, 3.0 g/l of yeast extract and 5.0 g/l of peptone (Ferreira et al 2011). The flasks were incubated in a rotatory shaker (Innova 4000 Incubator Shaker, New Brunswick Scientific, Enfield, CT, USA) at 200 rpm and 30 °C for 24 h. Following 24 h growth, broth was centrifuged (2,777.1× g for 10 min) and inoculum was prepared corresponding to 1.0 g/l cells (d. wt).…”

Section: Methodsmentioning

confidence: 99%

“…Considering that yeasts require a variety of substances for the synthesis of cellular material and energy (such as carbon, oxygen, nitrogen, etc. ), the presence of these nutrients is essential for a good performance of a microorganism in fermentation processes (Ferreira et al 2011). Aiming to replace the conventional and expensive nitrogen sources such as yeast extract and peptone in fermentation processes, RBE was used as a nitrogen source in the sugarcane bagasse hemicellulosic hydrolysate to produce economic ethanol by the yeast S. stipitis .…”

Section: Fermentation Of Hydrolysatementioning

confidence: 99%

Rice bran extract: an inexpensive nitrogen source for the production of 2G ethanol from sugarcane bagasse hydrolysate

et al. 2012

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Selection of the raw material and its efficient utilization are the critical factors in economization of second generation (2G) ethanol production. Fermentation of the released sugars into ethanol by a suitable ethanol producing microorganism using cheap media ingredients is the cornerstone of the overall process. This study evaluated the potential of rice bran extract (RBE) as a cheap nitrogen source for the production of 2G ethanol by Scheffersomyces (Pichia) stipitis NRRL Y-7124 using sugarcane bagasse (SB) hemicellulosic hydrolysate. Dilute acid hydrolysis of SB showed 12.45 g/l of xylose and 0.67 g/l of glucose along with inhibitors. It was concentrated by vacuum evaporation and submitted to sequential detoxification (neutralization by calcium hydroxide and charcoal adsorption). The detoxified hydrolysate revealed the removal of furfural (81 %) and 5-hydroxymethylfurfural (61 %) leading to the final concentration of glucose (1.69 g/l) and xylose (33.03 g/l). S. stipitis was grown in three different fermentation media composed of detoxified hydrolysate as carbon source supplemented with varying nitrogen sources i.e. medium #1 (RBE + ammonium sulfate + calcium chloride), medium #2 (yeast extract + peptone) and medium #3 (yeast extract + peptone + malt extract). Medium #1 showed maximum ethanol production (8.6 g/l, yield 0.22 g/g) followed by medium #2 (8.1 g/l, yield 0.19 g/g) and medium #3 (7.4 g/l, yield 0.18 g/g).

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“…Another strategy to reduce the toxic effect of inhibitors present in the hydrolyzate is high cell concentration, contributing towards a high number of viable cells for the formation of biomass and product (Ferreira, Mussatto, Cadete, Rosa, & Silva, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media

2017

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ABSTRACT. Biomass from lignocellulosic material constitutes a promising energy alternative and without competing with food production. However, pretreatments are required for conversion into sugars which release hexoses, pentoses and other sugars, coupled to inhibitors. Current analysis focuses on ethanol production with the three major inhibitors of lignocellulosic biomass pretreatment, namely, acetic acid, furfural and 5-hydroxymetilfurfural (HMF), and investigates the influence of a mixture of these inhibitors on fermentation by Pichia stipits, using commercial xylose as the only carbon source, through a full factorial 2 3 + 3 design of experiments (DOE). Fermentations were conducted in a laboratory scale, at 150 rpm and 72h, in a complex culture media with xylose and different inhibitor concentrations, based on the experimental analysis of sugarcane bagasse and 2.10 .

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Ethanol production by a new pentose‐fermenting yeast strain, Scheffersomyces stipitis UFMG‐IMH 43.2, isolated from the Brazilian forest

Cited by 50 publications

References 17 publications

Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis

Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis

Rice bran extract: an inexpensive nitrogen source for the production of 2G ethanol from sugarcane bagasse hydrolysate

<b>Effect of inhibitors on ethanol production by <i>Pichia stipitis</i> in a complex culture media

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