2020
DOI: 10.1080/2314808x.2020.1746884
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Ethanol production from cassava starch by protoplast fusants ofWickerhamomyces anomalusandGalactomyces candidum

Abstract: The potential of protoplast fusants of Wickerhamomyces anomalus and Galactomyces candidum isolates for ethanol production from cassava starch was evaluated. The protoplasts were obtained from 18 h yeast cells digested by zymolase and fused by polyethylene glycol (PEG). Regenerated recombinants were randomly selected and examined for desired marker traits: rate of fermentation of glucose, maltose and ethanol tolerance. Cassava starch hydrolysates were obtained by the amylolytic action of partially purified amyl… Show more

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Cited by 7 publications
(4 citation statements)
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“…In most cases, the ethanol titre is affected by the microbial tolerance to ethanol as a stress response (Gibson et al, 2007). The fermentation of cassava starch by subjecting the amylolytic hydrolysates produced from Aspergillus fermentation to ethanol production using protoplast fusion recombinants of yeast strains showed superior tolerance to a higher ethanol concentration of up to 25% v/v (Adeleye et al, 2020). On the other hand, use of butanol as a biofuel has remarkable advantages over ethanol in terms of higher energy, low volatility and can be mixed or completely replace current fuel systems (Green, 2011).…”
Section: Cassava Root Tubers For Bioenergymentioning
confidence: 99%
“…In most cases, the ethanol titre is affected by the microbial tolerance to ethanol as a stress response (Gibson et al, 2007). The fermentation of cassava starch by subjecting the amylolytic hydrolysates produced from Aspergillus fermentation to ethanol production using protoplast fusion recombinants of yeast strains showed superior tolerance to a higher ethanol concentration of up to 25% v/v (Adeleye et al, 2020). On the other hand, use of butanol as a biofuel has remarkable advantages over ethanol in terms of higher energy, low volatility and can be mixed or completely replace current fuel systems (Green, 2011).…”
Section: Cassava Root Tubers For Bioenergymentioning
confidence: 99%
“…The rising demand for biofuel offers countries like Nigeria opportunity for economic revolution in the production of biofuel from cassava. Adeleye et al [18] has report an ethanol yield of 1.5 L of 78% (v/v) from 2.5 kg wet weight of cassava. Countries like South Africa and China are already at advance stages of developing cassava plantations for production of starch for industrial uses [19,20].…”
Section: Bioethanolmentioning
confidence: 99%
“…Cassava starch is being used in beer making, ethanol production [24,25], pharmaceuticals, paper manufacturing, textile etc. [26].…”
Section: Fertilizer Influence On Starch Qualitiesmentioning
confidence: 99%