Ethanol production from Kinnow mandarin (Citrus reticulata) peels via simultaneous saccharification and fermentation using crude enzyme produced by Aspergillus oryzae and the thermotolerant Pichia kudriavzevii strain

Sandhu, Simranjeet Kaur; Oberoi, Harinder Singh; Dhaliwal, Sandeep Singh; Babbar, Neha; Kaur, Ujjal; Nanda, Dhiraj Kumar; Kumar, Dinesh

doi:10.1007/s13213-011-0302-x

Cited by 34 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Glucose, xylose, arabinose, glycerol, xylitol and oligosaccharides were determined with the high-performance liquid chromatograph (HPLC) [Dionex Corporation, Sunnyvale, CA, USA] using a Shodex SP-0810 column (300 9 7.8 mm) fitted with a SP-G guard column (Waters Inc., Milford, MA, USA). Ethanol was determined with the YSI 2786 ethanol membrane kits using YSI 2700 Select biochemical analyzer (YSI Inc, Buffalo, NY, USA) [29]. The other parameters, such as temperatures of column oven and refractive index detector were maintained as per the previously described procedure [29].…”

Section: Methodsmentioning

confidence: 99%

“…Details about the isolation, identification, and propagation of the yeast isolate are discussed in detail in our previous papers [10,29]. Extraction of genomic DNA was done with the fungal genomic DNA isolation kit (Chromous Biotech Pvt Ltd, Bangalore, India).…”

Section: Isolation and Propagation Of Yeast Cellsmentioning

confidence: 99%

“…Previous studies have reported the use of Pichia kudriavzevii (Issatchenkia orientalis) along with Saccharomyces cerevisiae in fermentation of grape must and sugarcane juice syrup [11,15]. We had recently reported ethanol production from sugarcane juice and Kinnow peel using P. kudriavzevii [10,29], but we are yet to come across any literature reporting the use of P. kudriavzevii for ethanol production from any lignocellulosic biomass. The present study was thus conducted to analyze the effect of different concentrations of sodium hydroxide (NaOH) on quantitative changes in the rice straw structural components; compositional changes during hydrolysis of pretreated rice straw; characterization of the P. kudriavzevii HOP1 strain; comparative evaluation of P. kudriavzevii and the conventional S. cerevisiae in ethanol production from glucose and also to evaluate the potential of P. kudriavzevii HOP1 in ethanol production from alkalitreated rice straw in a SSF process.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ethanol production from alkali-treated rice straw via simultaneous saccharification and fermentation using newly isolated thermotolerant Pichia kudriavzevii HOP-1

Oberoi

Babbar

Sandhu

et al. 2012

Journal of Industrial Microbiology and Biotechnology

Self Cite

105

View full text Add to dashboard Cite

In this study, simultaneous saccharification and fermentation (SSF) was employed to produce ethanol from 1% sodium hydroxide-treated rice straw in a thermostatically controlled glass reactor using 20 FPU gds⁻¹ cellulase, 50 IU gds⁻¹ β-glucosidase, 15 IU gds⁻¹ pectinase and a newly isolated thermotolerant Pichia kudriavzevii HOP-1 strain. Scanning electron micrograph images showed that the size of the P. kudriavzevii cells ranged from 2.48 to 6.93 μm in diameter while the shape of the cells varied from oval, ellipsoidal to elongate. Pichia kudriavzevii cells showed extensive pseudohyphae formation after 5 days of growth and could assimilate sugars like glucose, sucrose, galactose, fructose, and mannose but the cells could not assimilate xylose, arabinose, cellobiose, raffinose, or trehalose. In addition, the yeast cells could tolerate up to 40% glucose and 5% NaCl concentrations but their growth was inhibited at 1% acetic acid and 0.01% cyclohexamide concentrations. Pichia kudriavzevii produced about 35 and 200% more ethanol than the conventional Saccharomyces cerevisiae cells at 40 and 45°C, respectively. About 94% glucan in alkali-treated rice straw was converted to glucose through enzymatic hydrolysis within 36 h. Ethanol concentration of 24.25 g l⁻¹ corresponding to 82% theoretical yield on glucan basis and ethanol productivity of 1.10 g l⁻¹ h⁻¹ achieved using P. kudriavzevii during SSF hold promise for scale-up studies. An insignificant amount of glycerol and no xylitol was produced during SSF. To the best of our knowledge, this is the first study reporting ethanol production from any lignocellulosic biomass using P. kudriavzevii.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Isolation and Propagation Of Yeast Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ethanol production from alkali-treated rice straw via simultaneous saccharification and fermentation using newly isolated thermotolerant Pichia kudriavzevii HOP-1

Oberoi

Babbar

Sandhu

et al. 2012

Journal of Industrial Microbiology and Biotechnology

Self Cite

105

View full text Add to dashboard Cite

show abstract

“…The enzymes considered include -amylase (Bogar et al, 2002;Farid and Shata, 2011;Kareem et al, 2009;Pengthamkeerati et al, 2012;Sivaramakrishnan et al, 2007;Xu et al, 2008), protease (Chutmanop et al, 2008), and xylanase (Pirota et al, 2013;Szendefy et al, 2006), amongst others. However, to the best of our knowledge, there have been only a few studies concerning the production of cellulase by A. oryzae cultivated under SSF, from the bioprocess development point of view (Begum and Alimon, 2011;Lio and Wang, 2012;Sandhu et al, 2012). Given the potential of A. oryzae and its recognized application for industrial enzyme production, there is great interest in the characterization of this fungus in terms of cellulase production using SSF under controlled operational conditions.…”

Section: Introductionmentioning

confidence: 99%

BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION

Pirota

Tonelotto

Delabona

et al. 2016

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

-Bioprocess development studies concerning the production of cellulases are of crucial importance due to the significant impact of these enzymes on the economics of biomass conversion into fuels and chemicals. This work evaluates the effects of solid-state fermentation (SSF) operational conditions on cellulase production by a novel strain of Aspergillus oryzae using an instrumented lab-scale bioreactor equipped with an on-line automated monitoring and control system. The use of SSF cultivation under controlled conditions substantially improved cellulase production. Highest production of FPase (0.40 IU g -1 ), endoglucanase (123.64 IU g -1 ), and -glucosidase (18.32 IU g -1 ) was achieved at 28 C, using an initial substrate moisture content of 70%, with an inlet air humidity of 80% and an airflow rate of 20 mL min -1 . Further studies of kinetic profiles and respirometric analyses were performed. The results showed that these data could be very useful for bioprocess development of cellulase production and scale-up.

show abstract

“…omtA의 증폭은 forward primer, 5'-CAG GAT ATC ATT GTG GAC GG-3'와 reverse primer, 5'-CTC CTC TAC CAG TGG CTT CG-3' 를 사용하였고 [10], aflR은 F2 (5'-CCG GCG CAT AAC ACG TAC TC-3')와 R2 (5'-GGC GCT TGG CCA ATA GGT TC-3')를 사용하였다 [11]. [22][23][24]. P. kudriavzevii는 일반 효모와 달리 균의 모양이 길쭉하며, 경우 에 따라서는 세포질이 연결된 위균사(pseudohyphae)를 만 들기도 한다 (Fig.…”

unclassified

Diversity, Saccharification Capacity, and Toxigenicity Analyses of Fungal Isolates in Nuruk

김민식¹,

김신일²,

하병석³

et al. 2014

The Korean Journal of Mycology

View full text Add to dashboard Cite

: Nuruk samples collected from various regions in Korea were investigated in terms of fungal contents and diversity. In measurement of colony forming unit (CFU) in Nuruk suspensions on DRBC agar, Nuruk samples MS4, MS8, and MS10 were among the highest fungal density, with 1,278.9±21.6 (×10 ) were among the samples showing the highest fungal density. CFU per 20 mg Nuruk, respectively. The majority of fungal components were yeasts, including Pichia anomala, P. kudriavzevii, Kluyveromyces marxianus, and Saccharomycopsis fibuligera, whereas Aspergillus oryzae and Rhizopus oryzae, the representative Nuruk fungi, were predominant only in the low fungal density Nuruks (MS2, MS5, and MS11). Saccharification capability of the fungal isolates was assessed by measurement of amylase activity in the culture broth. The highest amylase activity was found in A. niger and A. luchuensis, followed by S. fibuligera. A. oryzae and R. oryzae showed fair amylase activity but significantly lower than those of the three fungal species. R. oryzae was suggested to play an additional role in degradation of β-glucan in crop component of Nuruk since R. oryzae was the only fungus that showed β-glucanase activity among the fungal isolates. To confirm the safety of Nuruk, aflatoxigenicity of the isolated Aspergillus was estimated using the DNA markers norB-cypA, aflR, and omtA. All of the isolates turned out to be non-aflatoxigenic as evidenced by the deletion of gene markers, norB-cypA and aflR, and the absence of aflatoxin in the culture supernatants shown by TLC analysis.

show abstract

Ethanol production from Kinnow mandarin (Citrus reticulata) peels via simultaneous saccharification and fermentation using crude enzyme produced by Aspergillus oryzae and the thermotolerant Pichia kudriavzevii strain

Cited by 34 publications

References 33 publications

Ethanol production from alkali-treated rice straw via simultaneous saccharification and fermentation using newly isolated thermotolerant Pichia kudriavzevii HOP-1

Ethanol production from alkali-treated rice straw via simultaneous saccharification and fermentation using newly isolated thermotolerant Pichia kudriavzevii HOP-1

BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION

Diversity, Saccharification Capacity, and Toxigenicity Analyses of Fungal Isolates in Nuruk

Contact Info

Product

Resources

About