Ether-Soluble &amp; Ether-Insoluble Auxins from Immature Corn Kernels

Srivastava, B. I. Sahai

doi:10.1104/pp.38.4.473

Cited by 22 publications

(8 citation statements)

References 6 publications

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“…Similarly, initial studies of auxin levels in maize showed high levels with apolar solvents such as diethyl ether or chloroform (Avery and others 1941), likely due to enzymes active within entrapped aqueous micellar droplets. Thus, as the enzyme age materialized in the early 1950s, more polar extraction solvents such as acetone, methanol, and ethanol were used to suppress enzyme activity during extraction (Hamilton and others 1961;Srivastava 1963;Wildman and Muir 1949). Acetone was initially used, but quenched radioactivity, and did not allow UV spectral analysis, so several groups tended toward primary alcohols.…”

Section: Purification Of Auxins and Their Measurementmentioning

confidence: 98%

Analytical History of Auxin

Tivendale

Cohen

2015

J Plant Growth Regul

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Auxins are a group of naturally occurring phytohormones that have numerous functions in plant growth and development. The identification and quantification of these hormones from plant tissues has a long and varied history, dating back as far as the mid-19th century. The evolution of auxin analysis closely mirrors the evolution of analytical chemistry. However, despite the fact that the methods for auxin determination have evolved since the initial bioassays, few of the techniques developed over the years have been completely abandoned, and many of them are still used in routine auxin analyses today.

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Section: Purification Of Auxins and Their Measurementmentioning

confidence: 98%

Analytical History of Auxin

Tivendale

Cohen

2015

J Plant Growth Regul

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“…Auxin activity was recorded between /?, 0,2-0,4 on the paper chromatograms, the highest activity being present in the stolons (Figure 2), Hemberg (1958) and Srivastava (1963) previously reported that IAA is found in this region on paper chromatograms. Expressed as IAA equivalents, the stolons contained about twice as much auxin as the sprouts (Table 1).…”

Section: Resultsmentioning

confidence: 60%

A Comparison of the Endogenous Hormone Levels in Stolons and Sprouts of the Potato Solanum tuberosum

Dimalla

Vanstaden

Smith

1977

Physiologia Plantarum

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Whereas the gibberellin and inhibitor levels of potato (Solanum tuberosum L. cv. BP‐1) sprouts and stolons differ, their auxin and cytokinin levels apparently remain more or less unchanged. The levels of gibberellin in the sprouts is higher than that of the stolons; however, with regard to inhibitor levels the reverse is true. It would appear as if an internal balance of different hormones controls whether or not tuberization will occur. High gibberellin and low inhibitor levels (sprouts) may favour cell elongation and at the same time limit cell division. Decreased gibberellin and increased inhibitor levels (stolons) seems to favour cell division. As cell division is not retarded despite high inhibitor levels, it would appear as if the effect of the inhibitor is mainly directed at countering the gibberellins.

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“…Hemberg et Tillberg (1980) ont rapporté que la quantité d'AIA extraite à partir du maïs sec avec l'éther diéthylique augmente continuellement avec le temps d'extraction. Srivastava (1963) a expliqué cette augmentation, soit par la conversion d'autres indoles, soit par l'hydrolyse progressive de l'AIA présente sous forme conjuguée. L'extraction par l'acétone donne une faible quantité d'AIA qui n'augmente pas significativement avec la température et la durée d'extraction prolongée.…”

Section: Discussionunclassified

Production de composés indoliques rhizogènes par le ver de terre <i>Lumbricus terrestris</i>

Harti¹,

Saghi²,

Molina³

et al. 2001

Can. J. Zool.

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L'application in vitro de l'extrait brut total de vers (Lumbricus terrestris) à différentes dilutions entraîne une stimulation de la rhizogenèse des plantules de haricots (Phaseolus vulgaris). L'effet rhizogène observé est similaire à celui enregistré en présence de l'acide indole acétique, substance de croissance connue chez les végétaux et utilisée ici comme témoin à différentes concentrations. Le fractionnement de l'extrait brut de vers par chromatographie sur colonne a permis d'isoler trois groupes de fractions. Seul le groupe de fractions polaires présente un effet rhizogène notable; cet effet est cependant inférieur à celui observé respectivement en présence de l'extrait brut total de vers et de l'acide indole acétique. Les analyses de l'extrait brut de vers par chromatographie sur couches minces, utilisant des réactifs et des systèmes chromatographiques appropriés, ont révélé l'absence de l'acide indole acétique et la présence probable d'autres dérivés indoliques à comportement chromotographique neutre à basique. Les composés indoliques présumés identifiés sont le méthyl-tryptophane, la sérotonine et l'hydroxyle acide indole acétique. Les analyses par spectrométrie de masse couplées à la chromatographie en phase gazeuse, effectuées après fractionnement et purification du groupe de fractions rhizogènes, ont révélé la présence de l'hydroxyle acide indole carboxylique; celui-ci serait pré-sent sous forme de plusieurs isomères de position. Abstract: In vitro application of total gross extract of earthworms (Lumbricus terrestris) in diverse dilutions stimulates rhizogenesis in young bean plants (Phaseolus vulgaris).The observed effect is similar to that of indol acetic acid, a well-known growth enhancer in plants, used here as a control in various concentrations. Fragmentation of worm extract by column chromatography results in three groups of fractions. Only the polar group of fractions has a significant rhizogenous effect, which is, however, inferior to that observed in the presence of total gross extract of worms or of indol acetic acid. Gross extract analyses using thin layer chromatography, with appropriate chromatography systems and reagents, revealed that indol acetic acid is not present, but is probably replaced by other indol-derived substances that have a neutral to basic chromatographic behaviour. These presumed indol-derived substances are identified as methyltryptophane, serotonin, and hydroxy-indol acetic acid. Analyses using mass spectrometry combined with gas chromatography, following fragmentation and purification of the group of rhyzogenous fractions, have revealed the presence of hydroxy-indol carboxylic acid, which seems to take the form of several isomeres.

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Ether-Soluble & Ether-Insoluble Auxins from Immature Corn Kernels

Cited by 22 publications

References 6 publications

Analytical History of Auxin

Analytical History of Auxin

A Comparison of the Endogenous Hormone Levels in Stolons and Sprouts of the Potato Solanum tuberosum

Production de composés indoliques rhizogènes par le ver de terre <i>Lumbricus terrestris</i>

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