Mushrooms have wide geographical distribution, with greatest commercial importance, both in temperate areas and tropical regions of the world. The agarics mycota of tropical eco-regions of Bangaluru, Karnataka was surveyed in different seasons from 2019 to 2020 for collection and identification of fungal samples. Detailed macroscopic and microscopic study of fungal samples was identified as Pleurotus pulmonarius (Fr.) Quél. P. ostreatus (Jack.) P. Kumm. P. populinus O. Hilber & O. K. Mill. For the first time, P. pulmonarius (Fr.) Quél was causing stem decay in Mangifera indica L. was reported from India. New host record of P. populinus O. Hilber & O. K. Mill on Spathodea campanulata was reported from India. The 3 species of Pleurotus was reported for the first time from the study area of Bengaluru, Karnataka, India.