Etiology of urinary tract infection and drug resistance cases of uropathogenes

Bhatt, CP; Shrestha, Basudha; Khadka, Sachin; Swar, Sumita; Shah, Bibha; Pun, Kong-Pang

doi:10.3126/jkmc.v1i2.8150

Cited by 16 publications

(22 citation statements)

References 10 publications

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“…The results were in agreement with the ndings by many other investigators [28][29][30]. The close proximity of the vaginal and anal opening in the female and the absence of the prostatic uid which has the bactericidal property in the female may make them more susceptible to the UTIs [29,31]. The patients of age group (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) showed the highest signi cant growth i.e.…”

Section: Discussionsupporting

confidence: 92%

“…The close proximity of the vaginal and anal opening in the female and the absence of the prostatic uid which has the bactericidal property in the female may make them more susceptible to the UTIs [29,31]. The patients of age group (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) showed the highest signi cant growth i.e. 44.49% which was similar to the study by Yadav The overall prevalence of UTI was 32.29% in diabetic patients and 15.97% in non-diabetic patients.…”

Section: Discussionmentioning

confidence: 99%

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Higher prevalence of ESBL producing uropathogenic Escherichia coli among Diabetic patients from a Tertiary Care Hospital of Kathmandu

Nayaju

Upreti

Ghimire

et al. 2020

Preprint

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Background: The increased rate of urinary tract infection (UTI) in immunocompromised patients especially diabetic patients is a major public health problem in adults. Moreover, the infection with multidrug resistant strains producing extended spectrum beta-lactamase (ESBL) is a key obstacle in disease management among such vulnerable population. An immediate proper treatment depends on rapid diagnosis of UTI and screening of antimicrobial resistant pattern with highly sensitive methods which also reduces the possible urinary complications among the diabetic patients. Hence, this study was aimed to determine the occurrence of antibiotic resistant genes for β-lactamases; blTEM and blaCTX-M in uropathogenic Escherichia coli isolates from UTI suspected diabetic and non-diabetic patients. attendingMethods: A hospital-based cross-sectional study was conducted in Kathmandu Model Hospital in association with Central Department of Microbiology, TU from June to December 2018. A total of 1267 non-duplicate mid-stream urine specimens from diabetic and non-diabetic patients were obtained and processed immediately for isolation of uropathogens. The isolates were subjected for antibiotic susceptibility testing and ESBL confirmation. Finally, blaTEM and blaCTX-M ESBL genes were screened by using specific primers.Results: The overall prevalence of the urinary tract infection UTI was found to be 17.20%(218/1267) , out of which diabetic patients were significantly more infected with UTI accounting for 32.29%(31/96) as compared to non-diabetic persons, 15.97%(187/1171). A total of 221 bacterial were from 218 culture positive specimens in which E . coli was a most predominate one; 67.9%(150/221.). Forty-four percent (66/150) of the total E. coli was MDR and 37.33%(56/150) were ESBL producers. Among 56 isolates, 92.3%(12/13) from diabetic patents and 83.0% (44/53) were from non-diabetic patients. Furthermore, 84.85% of the screened ESBL producers were confirmed to possess either single or both of the blaTEM and blaCTX-M genes . The blaTEM and blaCTX-M genes were detected in 53.57% and 87.5% of the phenotypically ESBL confirmed E. coli .Conclusions: The UTI infection is an increasing problem in diabetic patients and infection with multidrug resistant strains specially ESBL producing uropathogens are causing a huge problem in disease management leading to high rate of mortality and morbidity of diabetic patients.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Higher prevalence of ESBL producing uropathogenic Escherichia coli among Diabetic patients from a Tertiary Care Hospital of Kathmandu

Nayaju

Upreti

Ghimire

et al. 2020

Preprint

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“…E. coli, including other enterobacteria, are likely to have caused infection after colonization of the periurethral area by gastrointestinal tract flora [27]. This accords with other studies [21, 22, 24, 28-34]. However, discords with following studies [35-38].…”

Section: Discussionmentioning

confidence: 54%

ESBL Production Among E. coli and Klebsiella spp. Causing Urinary Tract Infection: A Hospital Based Study

Shakya¹,

Shrestha²,

Maharjan³

et al. 2017

TOMICROJ

108

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Introduction:Increase in extended-spectrum β-lactamases (ESBL) producing microbes in recent years has led to limitations of treatment options. This study aimed to assess the prevalence of ESBL producing E. coli and Klebsiella spp. at a tertiary hospital in Nepal.Methods:A total of 2209 non-repetitive mid-stream urine (MSU) samples were collected during the study period (March to September 2014). Identification of the isolates was done by Gram's staining followed by biochemical tests. Antibiotic susceptibility testing was done by modified Kirby-Bauer disc diffusion method and interpretation was done following Clinical and Laboratory Standard Institute (CLSI) guidelines, 2013. ESBL screening among E. coli and Klebsiella spp. isolates were done using ceftriaxone, cefotaxime, ceftazidime and cefpodoxime. The confirmation was done by phenotypic disc diffusion test (combined disc method) using ceftazidime (30µg) and ceftazidime plus clavulanic acid (30/10µg), and cefotaxime (30µg) and cefotaxime plus clavulanic acid (30/10µg) disc as per CLSI guidelines.Results:A total of 451 samples showed significant bacteriuria with 365 (80.9%) E. coli, 17 (3.8%) Klebsiella pneumoniae and 3 (0.7%) Klebsiella oxytoca. Of 451 isolates, 236 (52.3%) were found MDR strains. By combined disk test, 33 (91.7%) E. coli and 3 (8.3%) Klebsiella spp. were found ESBL producers.Conclusion:Higher prevalence of ESBL producing E. coli and Klebsiella spp. was observed warranting prompt need of surveillance for effective management of such MDR strains.

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“…The sex wise distribution ( Figure 2) showed that females (86.97%) were more susceptible than males (13.04%). Out of 51 samples, 23 samples showed the growth of E. coli which were subjected to biofilm productions by Congo red agar method among which 16 (70%) isolates showed the formation of biofilms [15]. All the biofilm producing UPEC were subjected to antibiotic susceptibility test [16,17] which showed maximum resistant towards erythromycin (100%) followed by amoxicillin (87.5%), cefotaxime (75%), levofloxacin (63%) and nitrofurantoin (12.5%).…”

Section: Resultsmentioning

confidence: 99%

Antibiotic susceptibility pattern of biofilm forming uropathogenic Escherichia coli isolated from UTI infected patients of Koshi Zonal Hospital in Biratnagar, Nepal

2018

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Objectives: To investigate the prevalence and antibiotic resistance pattern of biofilm-forming Uropathogenic Escherichia coli (UPEC) from urine samples isolated from UTI infected patients of Koshi zonal hospital, Biratnagar.Methods: A total of 51 urine samples from urinary tract infected patients were collected from Koshi zonal hospital, Biratnagar in the period of July to August 2017. Following the isolation and identification of biofilm-forming uropathogenic Escherichia coli, antibiotic susceptibility test was performed by a modified Kirby-Bauer disc diffusion technique. The biofilm detection was done by Congo red agar method.Results: In the present study, 45% of the urine samples showed a predominant growth of E. coli, among which 70% of isolates exhibited positive biofilm formation. Biofilm forming isolates revealed 100%, 87.5%, 75%, 63% and 12.5% resistant to erythromycin, amoxicillin, cefotaxime, levofloxacin, and nitrofurantoin respectively. Approximately 87.5% of biofilm-forming isolates were found multi-drug resistant.Conclusion: The study revealed the major issue of UTI by E. coli which may be due to poor sanitation, not the proper cleanliness of genitals and unsafe sexual intercourse. Nitrofurantoin and levofloxacin were examined the most effective antibiotics for UPEC. BIBECHANA 16 (2019) 47-54

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Etiology of urinary tract infection and drug resistance cases of uropathogenes

Cited by 16 publications

References 10 publications

Higher prevalence of ESBL producing uropathogenic Escherichia coli among Diabetic patients from a Tertiary Care Hospital of Kathmandu

Higher prevalence of ESBL producing uropathogenic Escherichia coli among Diabetic patients from a Tertiary Care Hospital of Kathmandu

ESBL Production Among E. coli and Klebsiella spp. Causing Urinary Tract Infection: A Hospital Based Study

Antibiotic susceptibility pattern of biofilm forming uropathogenic Escherichia coli isolated from UTI infected patients of Koshi Zonal Hospital in Biratnagar, Nepal

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