Etude des glycoprotéines carboxyliques et sulfatées DE la sécrétion bronchique humaine

Havez, R; Deminatti, M; Roussel, P; Degand, Pierre; Randoux, A; Biserte, G

doi:10.1016/0009-8981(67)90223-9

Cited by 30 publications

(8 citation statements)

References 7 publications

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confidence: 99%

“…The majority of studies of respiratory tract mucins have been histochemical and radioautographic (Havez et al, 1967;Lamb & Reid, 1969;Sturgess & Reid, 1972). Respiratory expectorations in diseased states have also been extensively studied (Havez et al, 1967;Ryley & Brogan, 1968). Other investigators have examined tracheobronchial tissues for their ability to incorporate radioactivity into trichloroacetic acid-precipitable material after incubation in radioactively labelled culture medium (Formijne et al, 1964;Yeager et al, 1971).…”

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Biosynthesis of respiratory-tract mucins. Incorporation of radioactive precursors into glycoproteins by canine tracheal explants in vitro

Ellis¹,

Stahl²

1973

Biochemical Journal

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1. Canine tracheal explants, cultured in medium 199, actively incorporated radioactive precursors into secreted macromolecules in vitro. 2. Puromycin, 6-diazo-5-oxo-l-norleucine and ouabain markedly inhibited the incorporation of these precursors. 3. Exogenous glucosamine at concentrations above 20mm caused a greater than 50% inhibition of the incorporation of l-[G-(3)H]fucose and l-[U-(14)C]serine. 4. Carbohydrate content of the purified secretions was approximately 50% and consisted principally of galactose, N-acetylglucosamine, N-acetylgalactosamine, fucose and sialic acids. 5. Chromatography on DEAE-cellulose and Bio-Gel A-150m and equilibrium density-gradient centrifugation in a CsCl gradient confirmed the presence of mucous glycoproteins. 6. Electrophoresis on 1% agarose gels gave profiles that were identical with canine respiratory mucus obtained in vivo. 7. These results support the utility of the explant system for studies of respiratory secretions.

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Biosynthesis of respiratory-tract mucins. Incorporation of radioactive precursors into glycoproteins by canine tracheal explants in vitro

Ellis¹,

Stahl²

1973

Biochemical Journal

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“…Their presence has been demonstrated in the human saliva [24] but this source of contamination may be controlled well enough by a careful aspiration of saliva in the mouth. The role of small glands in the nasal cavities, the pharynx, the trachea should not be forgotten; indeed SGP have been isolated from human bronchial mucus [16]. The detection of sulfate esters in the mucous glands at the csogastric junction suggests an unavoidable factor of contami nation; the esophageal cardiac glands.…”

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confidence: 99%

“…If sulfate esters have been detected in the abnormal gastric mucosa [9,20,21,26,30] in intestinal meta plasia or carcinoma, histochemical reactions are negative in the normal con ditions [20. 21], On the other hand, there is a possible contamination from upper digestive secretions during the collection, more especially as SGP are present in the human saliva [24] and in the human bronchial mucus [16]. The present work is an attempt to elucidate this problem by putting side by side some observations in patients: in vivo tracer studies with radiosulfate, collec tion of uncontaminated gastric juice and exploration of other sources of contamination of the gastric juice.…”

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Origin of the Sulfated Glycoproteins in Human Gastric Secretions

Lambert¹,

André²,

Bérard³

1971

Digestion

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While quantitative or immunological studies of sulfated glycoproteins have been carried out in the gastric juice, the actual origin of these molecules is far from being established; indeed sulfate is not detected histochemically in the normal gastric mucosa. Contamination by salivary glands and/or small glands in the pharynx, trachea and esophagus may be a cause of error. Some observations in patients throw light on the actual source of the SGP in gastric juice. In a patient treated for a melanoma, and receiving 3 injections of radiosulfate, the saliva contained a SGP of specific activity 685 000 dpm/mg. There was no histochemical or histoautoradiographical evidence of sulfate in fundic biopsies 6 and 24 h after the injection of the isotope. In 2 esophagectomized patients, there was no evidence of SGP in the juice obtained at gastrostomy, i.e. after interruption of the alimentary tract under the esophagogastric junction. At the same time SGP were present in the salivary secretions. The esophagogastric junction was examined in 2 specimens: a large amount of acidic glycoproteins was detected in the cardiac glands. Sulfate esters were detected mainly above the epithelial junction. Thus esophageal cardiac glands may be responsible for the presence of SGP in the gastric juice. Sulfate esters are also present in the submucosal glands of the thoracic esophagus. SGP in the gastric juice are not secreted by the normal gastric mucosa. Contamination by salivary SGP is more or less avoidable. The glands at the esophagogastric junction represent an unavoidable element of contamination.

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“…Calf tracheal cultures have been similarly used in studies of epithelial secretions (Kent, Molineux, Haegele & Stevenson, 1969). Histochemical and radioautographic studies have indicated that sulphate is incorporated into the mucusproducing structures of the tracheobronchial tree (Havez et al 1967;Havez & Biserte, 1968;Lamb & Reid, 1969;Chakrin, Baker, DeSanctis, Wardell & Spicer, 1970). The present study is concerned with the characterization of sulphated mucins secreted by these structures.…”

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Protection by zinc ions against the toxicity of cadmium ions

Webb¹

1971

Biochemical Journal

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Etude des glycoprotéines carboxyliques et sulfatées DE la sécrétion bronchique humaine

Cited by 30 publications

References 7 publications

Biosynthesis of respiratory-tract mucins. Incorporation of radioactive precursors into glycoproteins by canine tracheal explants in vitro

Biosynthesis of respiratory-tract mucins. Incorporation of radioactive precursors into glycoproteins by canine tracheal explants in vitro

Origin of the Sulfated Glycoproteins in Human Gastric Secretions

Protection by zinc ions against the toxicity of cadmium ions

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