Étude des γa globulines du colostrum humain

Human Secretory Immunoglobulins

“…The major IgA fraction of human secretions exhibits a sedimentation coefficient of about 11s (Tomasi et al 1965, Masson & Heremans 1966b, Rossen et al 1966b, Axelsson et al 1966, and its molecular weight has been estimated to be approximately 390,000 (Newcomb et al 1968, Tomasi & Czerwinski 1968, Hurlimann et al 1969). The same fraction has, on the other hand, been reported to be excluded from Sephadex G-200 gels (Rossen et al 1966a, Havez et al 1967 which retard globular proteins of molecular weights below 1,000,000 (Andrews 1966). In our experiments, however, salivary and colostral IgA were regularly retarded on Sephadex G-200 (Fig.…”

“…Whether this was due to specificity for the C characteristic of bound SP, or for the P determinant of polymer IgA, could not be established from their experiments. Havez et al (1967) mentioned that a conventional antiserum to SP reacted with macromolecules, probably IgA polymers, present in some pathological sera. In retrospect this might be ascribed to reactivity of the P determinant, since colostral IgA had been used for immunization.…”

Comparative Studies on Polymer‐Type Serum and Exocrine IgA

“…This finding confirms and expands the data collected by Tomasi et al (1965). It is difficult to reconcile the findings of Havez et al (1967) with these findings. The very weak reaction which in our study a few A-myeloma globulins gave with absorbed anti-colostral IgA antiserum when tested in whole serum, remains unexplained and requires further analysis.…”

“…In the secretory IgA, Tomasi et al (1965) demonstrated specific antigenic determinants ascribed to an additional polypeptide chain, known as transport piece or secretory piece. Havez et al (1967) found indications that antigenic structures characteristic of secretory IgA could be present 'in the small class of macromolecular ya-globulins' of the seruma finding contradicted by the data collected by Tomasi et al (1965).…”