Etude enzymatique compar�e de champignons entomopathog�nes des genres Beauveria et Metarhizium

Duriez-Vaucelle, T.; Fargues, Jacques; Robert, Pierre; Popeye, R.

doi:10.1007/bf00505786

Cited by 10 publications

(2 citation statements)

References 9 publications

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“…It is therefore important to be able to characterize fungi at the intraspecific level. Morphological characteristics in culture are insufficient for this but recent studies have shown that characterization is possible by electrophoretic isoenzyme and immuno-electrophoretic techniques (Duriez-Vaucelle et al 1981;Fargues et al 1981).…”

Section: (D) Economics Safety and Quality Controlmentioning

confidence: 99%

Fungi as biological control agents of arthropods of agricultural and medical importance

Hall¹,

Papierok

1982

Parasitology

146

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SUMMARYThere are many species of fungi attacking terrestrial and aquatic insects of agricultural and medical importance. Of these, few species have received much attention with a view to their use as biological control agents. The mechanisms of infection have been comparatively well studied, although many questions remain to be answered. The knowledge so far obtained has shed some light on the reasons for host specificity. Temperature, above all relative humidity, and their interactions are the most important physical factors influencing infection of terrestrial insects by entomopathogenic fungi and these are discussed in relation to epizootiological events. In aquatic environments, temperature, salinity and organic pollution are the important factors. In addition to these physical factors, numerous biotic factors at the level of the insect and the fungal pathogen influence both infection and spread of disease in insect populations. The complexity of the interactions of the biotic and abiotic factors makes it extremely difficult to study the influence of any one of these. Virulence of fungal pathogens and its measurement are discussed, together with the shortcomings of present bioassay systems; virtually no laboratory bioassay system exists which has been designed to yield data meaningful in the field. Mass-production techniques are described as are their inherent problems and those of formulation and storage. Finally, the achievements of fungal control of insects in the field are reviewed. Thus far, several species are mass-produced and are in widespread use, two of which,Verticillium lecaniiandHirsutella thompsonii, have been commercialized. More studies, ecological, fundamental and developmental are required in this field to realize fully the potential of other candidate fungi.

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Section: (D) Economics Safety and Quality Controlmentioning

confidence: 99%

Fungi as biological control agents of arthropods of agricultural and medical importance

Hall¹,

Papierok

1982

Parasitology

146

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“…Symbols refer to the mortality of 3rd-instar M. melolontha larvae induced by B. brongniartii: 0 = 0-25%; * = 25-50%; 8 = 50-75% and 6 = 75-100% mortality specitic variation and can result in misinterpretations of the genetic basis of the organism. The enzymes examined in this study were both regulatory and nonregulatory ones, and were known to be polymorphic in entomopathogenic fungi (DECONTI et al, 1980;DURIEZ-VAUCELL et al, 1981;BOUCIAS et al, 1982;RIBA et al, 1986aRIBA et al, , 1986bBRIDGE et al, 1990).…”

Section: Discussionmentioning

confidence: 94%

Protein and isozyme patterns among isolates of Beauveria brongniartii with different virulence to European cockchafer larvae (Melolontha melolontha L.)

Reineke

Zebitz

1996

J Applied Entomology

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It was the purpose of this study to elucidate whether sodium dodecyl sulfate‐polyactylamide gel electrophoresis (SDS‐PAGE) of soluble proteins and isozyme analysis of intracellular enzymes are useful methods to distinguish between 31 Beauveria brongniartii isolates of different virulence to Melolontha melolontha larvae. Cluster and principle coordinate analysis of data from soluble proteins demonstrated a high level of intraspecific variation and little correlation with virulence to M. melolontha larvae. The isozymes of the esterase, acid phosphatase and malate dehydrogenase showed high activity and gave consistent band resolution, resulting in 18 different enzyme types, whereas the enzyme systems of the alkaline phosphatase, catalase, glutamate oxalacetate transaminase, peroxidase and superoxide dismutase were not suitable for further evaluation. Levels of intraspecific variation after isozyme analysis were lower than in total protein analysis. Although two of the four B. brongniartii isolates with the lowest virulence against M. melolontha larvae differed in their enzyme patterns from the other isolates, a clear distinction between groups of different virulent isolates was not possible. Thus, analysis of the isozyme patterns of intracellular enzymes could only be a first step in selecting more promising B. brongniartii pathotypes for development as biocontrol agents.

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Immunological characterization of the entomopathogenic hyphomycetes Beauveria and Metarhizium

Fargues

Duriez²,

Popeye

et al. 1981

Mycopathologia

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Etude enzymatique compar�e de champignons entomopathog�nes des genres Beauveria et Metarhizium

Cited by 10 publications

References 9 publications

Fungi as biological control agents of arthropods of agricultural and medical importance

Fungi as biological control agents of arthropods of agricultural and medical importance

Protein and isozyme patterns among isolates of Beauveria brongniartii with different virulence to European cockchafer larvae (Melolontha melolontha L.)

Immunological characterization of the entomopathogenic hyphomycetes Beauveria and Metarhizium

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