EuNPs-MAb fluorescent probe based immunochromatographic strip for rapid and sensitive detection of fluorene

Development of a rapid and sensitive immunochromatographic strip based on EuNPs-ES fluorescent probe for the detection of early Trichinella spiralis-specific IgG antibody in pigs

“…EuNPs were conjugated with ES antigens as follows [ 29 ]: firstly, 10 μL of EuNPs were centrifuged at 14 000× g for 15 min to remove glycerol and phosphate, and then 10 µL of 1 mg/mL EDC, 10 µL of 1 mg/mL NHS, 100 µL of MES and EuNPs were mixed and stirred for 45 min to activate the EuNPs completely at room temperature. After removing the unbound EDC and NHS by centrifugation at 14 000 × g for 15 min, 20 µg the ML-ES and 20 µg PAW-ES antigens were mixed into the solution, and incubated for 3 h. Then, 200 µL of 5% BSA was added to the mixtures to block the unreacted active sites overnight at 4 °C.…”

The application of nanoparticles in point-of-care testing (POCT) immunoassays

“…153 Europium ions are also doped into polystyrene microspheres or silicon spheres, and then these particles are functionalized with carboxyl groups for conjugation with proteins. Eu-doped polystyrene microspheres have been applied in the detection of aflatoxins in feed, 154 silk fibroin in ancient silk, 155 clenbuterol in urine, 148 total thyroxine in serum, 156 polycyclic aromatic hydrocarbons in water, 157 antibodies in serum, 158 and even E. coli. O157:H7.…”

Development of a fluorescent immunoassay strip for the rapid quantitative detection of cadmium in rice

“…The fluorescent immunoassay test strip is based on the reaction between Cd and the coated immunogen Cd-ITCBE-BSA 5 with the fluorescent microsphere-labeled mAb (Qiao et al, 2018). Briefly, following the addition of 2 mL of fluorescently labelled mAb and 100 mL of Cd-containing sample to a well of a microtiter plate, we mixed the solution thoroughly within 5 min.…”