Evaluating effect of acrylamide and ascorbic acid on oxidative stress and apoptosis in ovarian tissue of wistar rat

Firouzabadi, Amir Masoud; Imani, Maryam; Zakizadeh, Fatemeh; Ghaderi, Nadia; Zare, Fatemeh; Yadegari, Maryam; Pourentezari, Majid; Fesahat, Farzaneh

doi:10.1016/j.toxrep.2022.07.015

Cited by 13 publications

(12 citation statements)

References 38 publications

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“…This gene is a protective factor against oxidative stress damage, so its up-regulation in the FA group indicates the external induction of oxidative stress. Similarly, several studies have reported that KL can attenuate oxidative damage and apoptosis [ 24 , 25 ].…”

Section: Resultsmentioning

confidence: 96%

Effect of Formaldehyde and Curcumin on Histomorphological Indices, Gene Expression Associated with Ovarian Follicular Development, and Total Antioxidant to Oxidant Levels in Wistar Rats

Farshad

Shahedi

Fesahat

et al. 2023

International Journal of Biomaterials

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The present experimental study was undertaken to investigate the effect of formaldehyde (FA) and curcumin (CUR) on histomorphological features, antioxidant potential, and messenger ribonucleic acid (mRNA) levels of genes related to follicular development in FA-exposed rats. 24 Wistar female rats were divided into four study groups and given intraperitoneal injections of FA (10 mg/kg) (N = 6), FA (10 mg/kg) + CUR (100 mg/kg) (N = 6), sham (N = 6), and control (N = 6) for 14 days. Ovarian follicular histology, the related gene expression, blood factors, and anti/oxidation potentials were assessed using ovarian tissue and serum, respectively. The klotho was significantly overexpressed in the FA group compared with controls and shams. Contradictory, the factor in germ line alpha was significantly down-regulated in FA and FA + CUR groups compared to shams and controls. A significant decline was seen in the number of ovarian follicles in the FA group, independent of the developmental stage. Regarding the comparison of the FA + CUR group to other groups, a significant change was seen in the number of secondary, graafian, and atretic follicles. The FA group demonstrated significantly lower hemoglobin, red blood cell count, hematocrit, and mean corpuscular hemoglobin concentration than controls. The activity of glutathione peroxidase increased significantly in the FA group than in the controls. Despite the deleterious effects of FA on histological and molecular aspects of rat ovarian follicles, CUR does not appear to have a protective effect against the hazardous effects of this chemical. However, CUR in some cases has positive effects such as reducing follicular destruction and interstitial edema.

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Section: Resultsmentioning

confidence: 96%

Effect of Formaldehyde and Curcumin on Histomorphological Indices, Gene Expression Associated with Ovarian Follicular Development, and Total Antioxidant to Oxidant Levels in Wistar Rats

Farshad

Shahedi

Fesahat

et al. 2023

International Journal of Biomaterials

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“…Oxidative stress response was activated by all chemicals in the ARE- bla and the AREc32 assay (Table and Figures S2 and S4), which is in line with previous studies which identified acrylamide as an activator of oxidative stress response in vitro , and in vivo . NMBA showed the strongest effect in both assays.…”

Section: Resultsmentioning

confidence: 99%

Reactivity of Acrylamides Causes Cytotoxicity and Activates Oxidative Stress Response

Huchthausen

Escher

Grasse

et al. 2023

Chem. Res. Toxicol.

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Acrylamides are widely used industrial chemicals that cause adverse effects in humans or animals, such as carcinogenicity or neurotoxicity. The excess toxicity of these reactive electrophilic chemicals is especially interesting, as it is mostly triggered by covalent reactions with biological nucleophiles, such as DNA bases, proteins, or peptides. The cytotoxicity and activation of oxidative stress response of 10 (meth)acrylamides measured in three reporter gene cell lines occurred at similar concentrations. Most acrylamides exhibited high excess toxicity, while methacrylamides acted as baseline toxicants. The (meth)acrylamides showed no reactivity toward the hard biological nucleophile 2-deoxyguanosine (2DG) within 24 h, and only acrylamides reacted with the soft nucleophile glutathione (GSH). Second-order degradation rate constants (k GSH) were measured for all acrylamides with N,N′-methylenebis(acrylamide) (NMBA) showing the highest k GSH (134.800 M–1 h–1) and N,N-diethylacrylamide (NDA) the lowest k GSH (2.574 M–1 h–1). Liquid chromatography coupled to high-resolution mass spectrometry was used to confirm the GSH conjugates of the acrylamides with a double conjugate formed for NMBA. The differences in reactivity between acrylamides and methacrylamides could be explained by the charge density of the carbon atoms because the electron-donating inductive effect of the methyl group of the methacrylamides lowered their electrophilicity and thus their reactivity. The differences in reactivity within the group of acrylamides could be explained by the energy of the lowest unoccupied molecular orbital and steric hindrance. Cytotoxicity and activation of oxidative stress response were linearly correlated with the second-order reaction rate constants of the acrylamides with GSH. The reaction of the acrylamides with GSH is hence not only a detoxification mechanism but also leads to disturbances of the redox balance, making the cells more vulnerable to reactive oxygen species. The reactivity of acrylamides explained the oxidative stress response and cytotoxicity in the cells, and the lack of reactivity of the methacrylamides led to baseline toxicity.

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“…AA can damage the reproductive system by damaging normal Sertoli cells in male rats and the function of Leydig cells, as well as induce the abnormal secretion of testosterone and luteinizing hormone, resulting in abnormal sperm-related gene expression, decreasing the number of sperm to reduce the activity of sperm, and increasing the sperm deformity rate [49,50]. Further, AA can induce ovarian dysfunction in female Wistar rats by upregulating apoptosis-related genes [51]. d. Other toxicities: AA can damage the liver, kidneys, lungs, bladder, and digestive tract and may even cause testicular mesothelioma, adrenal cortical adenoma, astrocytoma, and oral tumors [52].…”

Section: Potential Hazards Of Aamentioning

confidence: 99%

Production and Inhibition of Acrylamide during Coffee Processing: A Literature Review

Cao

2023

Molecules

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Coffee is the third-largest beverage with wide-scale production. It is consumed by a large number of people worldwide. However, acrylamide (AA) is produced during coffee processing, which seriously affects its quality and safety. Coffee beans are rich in asparagine and carbohydrates, which are precursors of the Maillard reaction and AA. AA produced during coffee processing increases the risk of damage to the nervous system, immune system, and genetic makeup of humans. Here, we briefly introduce the formation and harmful effects of AA during coffee processing, with a focus on the research progress of technologies to control or reduce AA generation at different processing stages. Our study aims to provide different strategies for inhibiting AA formation during coffee processing and investigate related inhibition mechanisms.

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Evaluating effect of acrylamide and ascorbic acid on oxidative stress and apoptosis in ovarian tissue of wistar rat

Cited by 13 publications

References 38 publications

Effect of Formaldehyde and Curcumin on Histomorphological Indices, Gene Expression Associated with Ovarian Follicular Development, and Total Antioxidant to Oxidant Levels in Wistar Rats

Effect of Formaldehyde and Curcumin on Histomorphological Indices, Gene Expression Associated with Ovarian Follicular Development, and Total Antioxidant to Oxidant Levels in Wistar Rats

Reactivity of Acrylamides Causes Cytotoxicity and Activates Oxidative Stress Response

Production and Inhibition of Acrylamide during Coffee Processing: A Literature Review

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