2018
DOI: 10.3389/fcimb.2018.00210
|View full text |Cite
|
Sign up to set email alerts
|

Evaluating New Compounds to Treat Burkholderia pseudomallei Infections

Abstract: Burkholderia pseudomallei is the causative agent of melioidosis, a disease that requires long-term treatment regimens with no assurance of bacterial clearance. Clinical isolates are intrinsically resistant to most antibiotics and in recent years, isolates have been collected that display resistance to frontline drugs. With the expanding global burden of B. pseudomallei, there is a need to identify new compounds or improve current treatments to reduce risk of relapse. Using the Pathogen Box generated by Medicin… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
29
0

Year Published

2019
2019
2024
2024

Publication Types

Select...
6
3

Relationship

0
9

Authors

Journals

citations
Cited by 35 publications
(30 citation statements)
references
References 34 publications
1
29
0
Order By: Relevance
“…MIC of 2–6 μg/ml for common B . pseudomallei isolates [ 38 40 ]). We evaluated the effectiveness of our assays using the Z’ statistic [ 34 ], commonly used for high-throughput screening (HTS) [ 41 ].…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…MIC of 2–6 μg/ml for common B . pseudomallei isolates [ 38 40 ]). We evaluated the effectiveness of our assays using the Z’ statistic [ 34 ], commonly used for high-throughput screening (HTS) [ 41 ].…”
Section: Resultsmentioning
confidence: 99%
“…We aimed to develop an assay that would identify compounds that reduced the proportion of B. thailandensis cells that remained viable when delivered in combination with ceftazidime (730 μM; 400 μg/ml; this is equivalent to the peak blood concentration given for melioidosis septicemia; c.f. MIC of 2-6 μg/ml for common B. pseudomallei isolates [38][39][40]). We evaluated the effectiveness of our assays using the Z' statistic [34], commonly used for high-throughput screening (HTS) [41].…”
Section: Assay Developmentmentioning
confidence: 99%
“…Compound 2 (IC 50 of 2.38 µM) is thought to be a DNA groove binder as likely mechanism of action, and is active against several bacterial and fungal pathogens [42]. Besides, this compound was among the seven MMV Pathogen Box compounds that exhibited bacteriostatic or bactericidal activity against Burkholderia pseudomallei , the causative agent of melioidosis, a disease that requires long-term treatment regimens with no assurance of bacterial clearance [43]. To a lesser extent, compound 3 also exhibited promising activity against E. histolytica (IC 50 5.63µM).…”
Section: Discussionmentioning
confidence: 99%
“…The persister frequency assay was performed as described in Ross et al (38). An overnight culture of B. cenocepacia K56-2 was subcultured to a concentration of 1×10 8 CFU/mL in 2 mL LB.…”
Section: Methodsmentioning
confidence: 99%