Evaluating polymeric biomaterial–environment interfaces by Langmuir monolayer techniques

Schöne, Anne-Christin; Roch, Toralf; Schulz, Burkhard; Lendlein, Andreas

doi:10.1098/rsif.2016.1028

Cited by 29 publications

(16 citation statements)

References 196 publications

(230 reference statements)

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“…Phospholipid monolayers are extensively used as a model for one leaflet of the cell membrane (Brockman 1999;Więcek et al 2008;Flasinski et al 2014). The surface concentration and charge density of the layer prepared on water surface can be easily adjusted by altering the composition and surface pressure of the monolayer (Kiss et al 2012;Hegedüs et al 2012;Pénzes et al 2012;Schöne et al 2017). The ordered lipid monolayer formed in a Langmuir trough or the supported lipid bilayers (Cranfield et al 2017;Marek et al 1999;Mohai et al 2002) obtained by liposome spreading or LB technique although much less complex than biological membranes are particularly suitable for studying the molecular interactions (Horvath et al 2013;Lohr et al 2014;Ábrahám et al 2016;Ábrahám et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Membrane affinity and fluorescent labelling: comparative study of monolayer interaction, cellular uptake and cytotoxicity profile of carboxyfluorescein-conjugated cationic peptides

et al. 2018

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Fluorescent labelling is a common approach to reveal the molecular details of cellular uptake, internalisation, transport, distribution processes in biological systems. The conjugation with a fluorescent moiety might affect relevant physico-chemical and in vitro transport properties of the bioactive component. A representative set of seven cationic peptides-including cell-penetrating peptides as well as antimicrobial peptides and synthetic derivatives-was selected for our comparative study. Membrane affinity of the peptides and their 5(6)-carboxyfluorescein (Cf) derivatives was determined quantitatively and compared applying Langmuir monolayer of zwitterionic (DPPC) and negatively charged (DPPC + DPPG) lipids as cell membrane models. The interaction with neutral lipid layer is mainly governed by the overall hydrophobicity of the molecule which is remarkably increased by Cf-conjugation for the most hydrophobic Magainin, Melittin and Transportan. A significantly enhanced membrane affinity was detected in negatively charged lipid model monolayer for all of the peptides since the combination of electrostatic and hydrophobic interaction is active in that case. The Cf-conjugation improved the penetration ability of Penetratin and Dhvar4 suggesting that both the highly charged character (Z/n) and the increased hydrophobicity by Cf-conjugation present important contribution to membrane interaction. This effect might also responsible for the observed high in vitro internalisation rate of Penetratin and Dhvar4, while according to in vitro studies they did not cause damage of cell membrane. From the experiments with the given seven cationic peptides, it can be concluded that the Cf-conjugation alters the degree of membrane interaction of such peptides which are moderately hydrophobic and highly charged.

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Section: Introductionmentioning

confidence: 99%

Membrane affinity and fluorescent labelling: comparative study of monolayer interaction, cellular uptake and cytotoxicity profile of carboxyfluorescein-conjugated cationic peptides

et al. 2018

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“…The isotherms presented the various regimes that can be ascribed to the OEG conformational transitions [7,19,20], by analogy to the behavior observed for diblock copolymers (for example, poly(styrene)-poly(ethylene oxide) [25]. At large molecular areas, liquid-expanded monolayers were observed in which the dendrons were anchored at the interface by the phenyl groups, while the OEG chains lay flat at this interface, forming a so-called “pancake” structure [19,26,27,28].…”

Section: Resultsmentioning

confidence: 99%

Interfacial Behavior of Oligo(Ethylene Glycol) Dendrons Spread Alone and in Combination with a Phospholipid as Langmuir Monolayers at the Air/Water Interface

et al. 2019

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Dendrons consisting of two phosphonate functions and three oligo(ethylene glycol) (OEG) chains grafted on a central phenoxyethylcarbamoylphenoxy group were synthesized and investigated as Langmuir monolayers at the surface of water. The OEG chain in the para position was grafted with a t-Bu end-group, a hydrocarbon chain, or a partially fluorinated chain. These dendrons are models of structurally related OEG dendrons that were found to significantly improve the stability of aqueous dispersions of iron oxide nanoparticles when grafted on their surface. Compression isotherms showed that all OEG dendrons formed liquid-expanded Langmuir monolayers at large molecular areas. Further compression led to a transition ascribed to the solubilization of the OEG chains in the aqueous phase. Brewster angle microscopy (BAM) provided evidence that the dendrons fitted with hydrocarbon chains formed liquid-expanded monolayers throughout compression, whilst those fitted with fluorinated end-groups formed crystalline-like domains, even at large molecular areas. Dimyristoylphosphatidylcholine and dendron molecules were partially miscible in monolayers. The deviations to ideality were larger for the dendrons fitted with a fluorocarbon end-group chain than for those fitted with a hydrocarbon chain. Brewster angle microscopy and atomic force microscopy supported the view that the dendrons were ejected from the phospholipid monolayer during the OEG conformational transition and formed crystalline domains on the surface of the monolayer.

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“…In Langmuir monolayer degradation experiments, ultrathin polymer films at the air–water interface are used to study the degradation behavior of polymer materials . A thin polymer film, when exposed to a degrading medium, is a direct representation of the surface of a degrading polymeric object.…”

Section: Methodological Approach: Langmuir Techniquesmentioning

confidence: 99%

Langmuir Monolayers as Tools to Study Biodegradable Polymer Implant Materials

Machatschek

Schulz

Lendlein

2018

Macromol. Rapid Commun.

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Langmuir monolayers provide a fast and elegant route to analyze the degradation behavior of biodegradable polymer materials. In contrast to bulk materials, diffusive transport of reactants and reaction products in the (partially degraded) material can be neglected at the air–water interface, allowing for the study of molecular degradation kinetics in experiments taking less than a day and in some cases just a few minutes, in contrast to experiments with bulk materials that can take years. Several aspects of the biodegradation behavior of polymer materials, such as the interaction with biomolecules and degradation products, are directly observable. Expanding the technique with surface‐sensitive instrumental techniques enables evaluating the evolution of the morphology, chemical composition, and the mechanical properties of the degrading material in situ. The potential of the Langmuir monolayer degradation technique as a predictive tool for implant degradation when combined with computational methods is outlined, and related open questions and strategies to overcome these challenges are pointed out.

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Evaluating polymeric biomaterial–environment interfaces by Langmuir monolayer techniques

Cited by 29 publications

References 196 publications

Membrane affinity and fluorescent labelling: comparative study of monolayer interaction, cellular uptake and cytotoxicity profile of carboxyfluorescein-conjugated cationic peptides

Membrane affinity and fluorescent labelling: comparative study of monolayer interaction, cellular uptake and cytotoxicity profile of carboxyfluorescein-conjugated cationic peptides

Interfacial Behavior of Oligo(Ethylene Glycol) Dendrons Spread Alone and in Combination with a Phospholipid as Langmuir Monolayers at the Air/Water Interface

Langmuir Monolayers as Tools to Study Biodegradable Polymer Implant Materials

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