Evaluating recipient and embryo factors that affect pregnancy rates of embryo transfer in beef cattle

Spell, A.R.; Beal, W. E.; Corah, L.R.; Lamb, G.C.

doi:10.1016/s0093-691x(01)00563-5

Cited by 127 publications

(137 citation statements)

References 25 publications

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“…However, whether the maintenance of a pregnancy after an embryo transfer increases with an increasing pre-ovulatory follicle size of the recipient is still under discussion (Atkins et al 2010;Jinks et al 2013). Because CL morphology and activity could be used to find good recipients for an embryo transfer (Spell et al 2001;Chagas e Silva et al 2002;Chebel et al 2008;Yoshida et al 2012), we support the hypothesis that follicle size could be a suitable prospective marker for identifying suitable recipients very early.…”

Section: Discussionsupporting

confidence: 60%

“…Parameters, which are suitable for predicting the individual fertility of a healthy animal in an individual cycle and which can be measured easily in clinical examinations like ultrasound, are very rare. Especially in order to find recipients for an embryo transfer, it is important to identify prospectively individual animals with the best chance of getting pregnant (Spell et al 2001;Chagas e Silva et al 2002;Chebel et al 2008;Yoshida et al 2012).…”

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confidence: 99%

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Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Vernunft¹,

Weitzel²,

Viergutz³

2013

Vet. Med. - Czech

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Secretion of adequate levels of progesterone from a proper corpus luteum (CL), which develops out of the cells of a healthy preovulatory follicle, is a key-factor for establishment of a pregnancy. The aim of this study was to investigate the relationship between morphological and secretory characteristics of preovulatory follicles and their corresponding corpus luteum with regard to the post-partum period in high-yielding dairy cows. Therefore, ultrasound-guided aspirations of preovulatory follicles were performed repeatedly, using 20 first lactating cows between 26 and 121 days after parturition. Heat was induced with a PGF analogon followed by administration of a GnRH analogon. The dominant follicle was aspirated 21 h after administration of the GnRH analogon. The diameters of the follicles were estimated at aspiration and the morphology of the resulting luteal tissue was examined on day 14 after follicle aspiration using ultrasonographic examinations. Concentrations of progesterone (P 4 ) and 17-beta-oestradiol (E 2 ) were determined in the follicular fluids (FF) and P 4 concentration was estimated at the time of CL examination in plasma. A CL development occurred in 82% after dominant follicle aspiration. The interval of time between parturition and follicle aspiration did not affect the investigated variables. The diameter of the aspirated preovulatory follicle was positively correlated to the cross-section area of the developed luteal tissue (R = 0.60; P < 0.01) as well as to the plasma P 4 concentration on day 14 after follicle aspiration (R = 0.47; P < 0.05). Also, E 2 concentrations in FF were positively correlated to cross-section area of the luteal tissue (R = 0.54; P < 0.05). Comparing the FF of the follicles that gave rise to a CL after aspiration to follicles that did not, both types had comparable P 4 , but the former type harboured higher E 2 concentrations. In conclusion, preovulatory follicle diameter as well as steroid concentrations in the follicular fluid could be used prospectively to identify cows which will have well-developed CLs and high plasma P 4 levels later. On the other hand, CL development after follicle aspiration can be used as a retrospective quality parameter of dominant follicles. These results will help to identify suitable animals for breeding or recipients for embryo transfer.Keywords: post-partum period; ultrasound; fertility; progesterone; oestradiol; follicle fluid List of abbreviations CL = corpus luteum, DM = dry matter, E 2 = 17-beta-oestradiol, FF = follicle fluid, GnRH = gonadotropin realising hormone, P 4 = progesterone, PGF = prostaglandin F2 alfa, TMR = total mixed ration

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Section: Discussionsupporting

confidence: 60%

mentioning

confidence: 99%

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Vernunft¹,

Weitzel²,

Viergutz³

2013

Vet. Med. - Czech

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“…Moreover, the success of ET depends on factors associated with the embryo (quality, developmental stage, fresh/frozen), the recipient (donor-recipient synchrony, progesterone concentrations), o r an interaction among both (Hasler, 2001;Spell et al, 2001). The objective of this study was to determine the factors affecting pregnancy rates following ET in Bos indicus and Bos taurus / Bos indicus cows raised under tropical conditions.…”

Section: Andmentioning

confidence: 99%

Factors Affecting Pregnancy Rate of Embryo Transfer inBos indicusandBos taurus/Bos indicuscows

Montiel

Galina

Rubio³

et al. 2006

Journal of Applied Animal Research

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“…A variety of factors may influence the pregnancy and live birth rates resulting from the transfer of frozen-thawed preimplantation embryos to synchronous recipient females 5 . Embryos at developmental stages less advanced than compact morula or more advanced than expanded blastocyst often do not survive the cryopreservation process as well as embryos at the compact morula, early blastocyst, blastocyst, and expanded blastocyst stages of embryonic development.…”

Section: Representative Resultsmentioning

confidence: 99%

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Youngs¹

2011

JoVE

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Preimplantation embryos from cattle, sheep, and goats may be cryopreserved for short-or long-term storage. Preimplantation embryos consist predominantly of water, and the avoidance of intracellular ice crystal formation during the cryopreservation process is of paramount importance to maintain embryo viability. Embryos are placed into a hypertonic solution (1.4 -1.5 M) of a cryoprotective agent (CPA) such as ethylene glycol (EG) or glycerol (GLYC) to create an osmotic gradient that facilitates cellular dehydration. After embryos reach osmotic equilibrium in the CPA solution, they are individually loaded in the hypertonic CPA solution into 0.25 ml plastic straws for freezing. Embryos are placed into a controlled rate freezer at a temperature of -6°C. Ice crystal formation is induced in the CPA solution surrounding the embryo, and crystallization causes an increase in the concentration of CPA outside of the embryo, causing further cellular dehydration. Embryos are cooled at a rate of 0.5°C/min, enabling further dehydration, to a temperature of -34°C before being plunged into liquid nitrogen (-196°C). Cryopreserved embryos must be thawed prior to transfer to a recipient (surrogate) female. Straws containing the embryos are removed from the liquid nitrogen dewar, held in room temperature air for 3 to 5 sec, and placed into a 37°C water bath for 25 to 30 sec. Embryos cryopreserved in GLYC are placed into a 1 M solution of sucrose for 10 min for removal of the CPA before transfer to a recipient (surrogate) female. Embryos cryopreserved in EG, however, may be directly transferred to the uterus of a recipient.

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Evaluating recipient and embryo factors that affect pregnancy rates of embryo transfer in beef cattle

Cited by 127 publications

References 25 publications

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Factors Affecting Pregnancy Rate of Embryo Transfer inBos indicusandBos taurus/Bos indicuscows

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

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