EVALUATING THE ACCUMULATION TREND OF L-DOPA IN DARK-GERMINATED SEEDS AND SUSPENSION CULTURES OF Phaseolus vulgaris L. BY AN EFFICIENT UV-SPECTROPHOTOMETRIC METHOD

Abstract

Recebido em 30/08/2017; aceito em 12/12/2017; publicado na web em 01/02/2018Seed germination and plant cell cultures provide an alternative mean for producing secondary metabolites. The present study is an attempt to evaluate the effect of seed dark germination and some elicitors and precursors on the production of L-DOPA in Phaseolus vulgaris L. Callus cultured on Murashige and Skoog medium supplemented with various concentrations of different plant growth regulators. L-DOPA produced was quantified by UV-spectrophotometric method. In this study, a user-friendly, quick, and economical UV-spectrophotometric method was described to determine L-DOPA content in extracts from 33 biotypes of Phaseolus vulgaris L. The method is based on the nitrosation of L-DOPA to form a yellow solution and then formation of a red solution by adding base which is measurable at 470 nm. According to our statistical studies, this method showed high efficiency and selectivity for quantitative determination of L-DOPA in herbal extracts from dried plant seeds, dark-germinated seeds and callus cultures. L-DOPA content in dark-germinated seeds and suspension cultures increased significantly to approximately several-fold compared to the control. The implication from this study is that elicitor treatment and precursor feeding of Phaseolus vulgaris L. can significantly improve the parkinson's relevant L-DOPA content.

State of the Art in Crystallization‐Induced Diastereomer Transformations

“…Indeed, a wet-milling ). [92] The procedure was inspired by data published for methyl 4chlorophenylalaninate, [93] however, 5-nitrosalicylaldehyde was pinpointed as a superior racemization catalyst this time. "Optical purities 78-88%" were reported for amino acids (S)-112 a-e.…”

“…utilized a Schiff base chemistry in CIDT of five phenylalanine derivatives 111 a – e , including esters of L‐tyrosine ( 111 a ) and of L‐Dopa ( 111 d ) (Scheme 41). [92] The procedure was inspired by data published for methyl 4‐chlorophenylalaninate, [93] however, 5‐nitrosalicylaldehyde was pinpointed as a superior racemization catalyst this time. “Optical purities 78–88%” were reported for amino acids ( S )‐ 112 a – e .…”

Proximate composition and anti-nutritional factors in Mucuna pruriens (velvet bean) seed flour as affected by several processing methods

“…l -DOPA content of the samples was determined using the procedure described by Rahmani-Nezhad et al [ 14 ]. Mucucna pruriens seed was purchased from New Market in Enugu State, Nigeria.…”

Content and Yield of L-DOPA and Bioactive Compounds of Broad Bean Plants: Antioxidant and Anti-Inflammatory Activity In Vitro

“…Analysis. The methodology proposed by Rahmani-Nezhad et al [53] was adapted to carry out the analysis in microplates. We placed 10 µL of the extract (5.7 mg mL −1 ) or a standard in a flat-bottom UV microplate and added 20 µL of 3% (p/v) NaNO 2 and 10 µL HCl at 1 M. The mixture was shaken and left in repose for 5 min; this turned yellow.…”