Evaluating the Efficacy of the Treatment in Lambs with Cryptosporidium spp. via Saa and Haptoglobin

Abstract

Background: The purpose of this study is to evaluate the efficacy of the treatment for the diarrhea due to Cryptosporidium, which is prevalent in lambs, via the concentrations of Serum Amyloid A (SAA) and Haptoglobin (Hp). Although there are experimental studies in this field, there are quite a few studies on the lambs which are infected naturally. Methods: In this study, stool samples were collected from the lambs that were affected with diarrhea and Cryptosporidium was detected via the rapid test kit (Bio-X Diagnostics S.A. Rochefort/Belgium). Following this procedure, 15 lambs were included in the study by examining same stool samples microscopically and 50 mg/kg of paromomycin sulphate (Huvepharma) was administered to the lambs in which Cryptosporidium spp. was detected for five days as a treatment. 10 healthy lambs formed the control group. Blood samples were taken from all lambs to measure SAA and Hp in the pre and post treatment stages. In the serums obtained from blood samples, concentrations of SAA and Hp were measured in ELISA by using commercial kits. Result: In the lambs with Cryptosporidium, concentrations of SAA and HP were as (SAA; 20,62±4,83 ng/mL HP; 2,63±0,77 µg/mL) in pre-treatment and as (SAA;12,93±3,27 ng/mL, Hp; 1,59±0,73 µg/mL) in post-treatment stage while they were as (SAA;10,07±2,49 ng/mL, Hp; 1,03±0,59 µg/mL) in the control group. In the pre and post treatment stage measurements, a statistically significant difference (p less than 0,05) in the values of SAA and Hp was found between the control group and the lambs wit diarrhea due to Cryptosporidium. It was suggested that this might have occurred as a result of inflammatory response against Cryptosporidium. In the light of these findings, it is asserted that routine measurements of SAA and Hp concentrations of the lambs with diarrhea due to cryptosporidiosis could be beneficial in the following up the treatment, determining the severity of the infection, choosing the right method for treatment and monitoring the efficacy of the chosen treatment method and in detecting subclinical diseases in veterinary medicine.

Efficacy of Paromomycin Sulphate for the Treatment of Cryptosporidiosis in Goat Kids

“…Demirbas and Kabu (2021) determined in their study on pneumonic goats that SAA concentration was higher in the pneumonic group than the healthy goats. Kabu et al, (2023), SAA and Hp concentrations were found high in lamb with diarrhea due to Cryptosporidium. Additionally, SAA and Hp concentrations were found high in goats with pregnancy toxemia (Albay et al, 2014).…”

“…Although there are many studies on the values of APPs in Neonatal Diarrhoea Syndrome in calves and lambs, but no literature is available on the level APPs in goat kids affected with Neonatal Diarrhoea Syndrome (Kabu et al, 2023;Kabu and Uyarlar, 2022;Dinler et al, 2017;Albayrak and Kabu, 2016). Thus, results of our study Indicate that APPs can be used as a potential diagnostic tool in veterinary medicine.…”

“…Neonatal Diarrhea Syndrome is a common issue in sheep and goat herds and Cryptosporidiosis, which also contributes to that syndrome, has been reported to be the one of the reasons of the enteric disease in humans and farm animals (Kabu et al, 2023;Feng and Xiao, 2017;Giadinis et al, 2012). Among the Crytospordium species, Cryptosporidium parvum, C. ubiquitum and C. xiaoiare are the dominant species in the sheep and goats.…”