Evaluating the protective efficacy of antigen combinations against Photobacterium damselae ssp. piscicida infections in cobia, Rachycentron canadum L.

Abstract

Cobia, Rachycentron canadum L., is a very important aquatic fish that faces the risk of infection with the bacterial pathogen Photobacterium damselae ssp. piscicida, and there are few protective approaches available that use multiple antigens. In the present study, potent bivalent antigens from P. damselae ssp. piscicida showed more efficient protection than did single antigens used in isolation. In preparations of three antigens that included recombinant heat shock protein 60 (rHSP60), recombinant α-enolase (rENOLASE) and recombinant glyceraldehyde-3-phosphate dehydrogenase (rGAPDH), we analysed the doses that elicited the best immune responses and found that this occurred at a total of 30 μg of antigen per fish. Subsequently, vaccination of fish with rHSP60, rENOLASE and rGAPDH achieved 46.9, 52 and 25% relative per cent survival (RPS), respectively. In addition, bivalent subunit vaccines--combination I (rHSP60 + rENOLASE), combination II (rENOLASE + rGAPDH) and combination III (rHSP60 + rGAPDH)--were administered and the RPS in these groups (65.6, 64.0 and 48.4%, respectively), was higher than that achieved with single-antigen administration. Finally, in combination IV, the trivalent vaccine rHSP60 + rENOLASE + rGAPDH, the RPS was 1.6%. Taken together, our results suggest that combinations of two antigens may achieve a better efficiency than monovalent or trivalent antigens, and this may provide new insights into pathogen prevention strategies.

Detecção, controle e prevenção de fotobacteriose em cultivo de bijupirá

“…piscicida é um patógeno responsável pela doença denominada fotobacteriose (também conhecida como pasteurelose ou pseudotuberculose), de grande importância na aquicultura por ser encontrado globalmente em espécies de peixes marinhos cultivados (Romalde, 2002;Ho et al, 2014), como R. canadum (Liao et al, 2004), olhete (Seriola quinqueradiata) (Romalde, 2002), pargo europeu (Sparus aurata) (Andreoni & Magnani, 2014) e robalo europeu (Dicentrarchus labrax) (Korun & Timur, 2005).…”

“…piscicida remontam ao final da década de 1980 e meados da década de 1990 (Romalde, 2014), e muito progresso ocorreu desde então, especialmente em virtude da uniformidade sorológica do patógeno, que facilitou o desenvolvimento de ferramentas de diagnóstico e também de vacinas específicas e eficientes (Thune et al, 2003;Romalde, 2014). Atualmente, existem vacinas específicas para P. damselae comercializadas por empresas internacionais (Gudding & Van Muiswinkel, 2013), além de pesquisas para o desenvolvimento de novas vacinas de segunda geração que prometem ser ainda mais eficientes (Ho et al, 2011(Ho et al, , 2014. Lin et al (2006) relataram produção de anticorpos uma semana após a injeção de P. damselae subsp.…”

Photobacteriosis: Prevention and Diagnosis

“…piscicida isolates [4]. Moreover, antigen combinations were studied revealing that bivalent subunit vaccines may achieve a better efficiency than monovalent or trivalent antigens [41]. …”

Detection of specific immune response in sole (Solea senegalensis) against Photobacterium damselae subsp. piscicida antigens

“…Several studies have reported the protection against P. damselae subsp. piscicida by different designs of vaccines in cultured fish [1][2][3][4][5]. Vaccines are usually composed of inactivated whole cells (bacterin), containing a wide pool of antigens.…”