In recent years, the number of underwater road tunnels in Chinese cities has increased. However, the current situation of personal fire safety literacy as it pertains to these tunnels remains unclear. To address this gap, a questionnaire survey was conducted to investigate people’s awareness of escape slides, evacuation signs, and the correct evacuation paths for fire escape. A total of 1049 respondents in Changsha, China, were surveyed, with 791 valid questionnaires collected and analyzed. The findings revealed that a significant proportion of respondents (81.80%) were unaware of the presence of escape slides in underwater road tunnels, while 87.86% could not recognize them and 93.05% could not use them. Only 42.04% of respondents could identify evacuation signs in underwater road tunnels. In the event of a fire, just half of the respondents could select the appropriate escape or evacuation path. Additionally, demographic differences among respondents also influenced their level of fire safety literacy. Based on these findings, it is recommended that the government and relevant organizations should enhance the dissemination of knowledge regarding escape slides and evacuation signs in underwater road tunnels.