Evaluation and Characterization of Bioactive Compound of Hamelia Patens Plant With the Gc-MS Spectroscopy

Abstract

Objective: The aim of this study is to evaluate the bioactive compound present in the leaves, root, flower, and stem by gas chromatography (GC) analysis. Novel drugs are isolated from plants against human diseases.Methods: The plant parts (leaves, stem, flower, and root) were collected, washed, shade dried, and powdered, and the methanol extracts of all plant parts were prepared by Soxhlet reflux method. The methanolic extracts were analyzed for the identification of phytochemical compounds present in the Hamelia patens plant parts using GC-mass spectrometry (MS) matched by the National Institute of Standards And Technology-11 library and Willey 8 library.Results: The bioactive fraction on GC-MS analysis revealed a chromatogram showing highest peaks. Methanol extracts of H. patens leaf are found to possess a wide range of saturated and unsaturated aliphatic hydrocarbons and their isomers such as 2,3-dihydro-3,5-dihydroxy-6-methyl- 4H-pyran (1.77%), 1,3-propanediol, 2-ethyl-2-(hydroxymethyl) (3.06%), mome inositol (18.22%), pentadecanoic acid (1.66%), and squalene (11.47%). GC-MS analysis revealed chromatogram, showing that the highest peaks of H. patens stem are methyl salicylate (3.41%), 2-amino-9-(3,4- dihydroxy-5- hydroxymethyl) (9.53%), mome inositol (63.73%), and squalene (1.07%).Conclusion: Isolation of such bioactives and their use as therapeutic target is the prime motto of our research to isolate novel bioactive that can used as potential therapeutic agents.

A Phytochemical and Ethnopharmacological Recapitulation on Hamelia patens

“…Ethanolic leaf extract of Hamelia was obtained using different method of extraction and analyzed by HPLC/ESI-MS method, in which various constituent were observed such as quinic acid, hydroxyl cinnamic acid, catechin, caffeoyl quinic acid, procyanidin B2, epicatechin, ( + )-catechin3-O-glucose, quercetin 3-O-rutinoside, kaempferol-3-O-rutinoside and 3-hydroxyphloretin-2'-O-glucoside [30]. GC-MS analysis showed that Hamelia contains major phytoconstituent which belongs to the different classes of natural products such as Momeinositol, Pentadecanoic acid, 9 octadecenol, Squalene, Gamma-sitosterol, Mitrajavine, Ergost 5-en-3-ol (3,beta), Stigmast-7-en-3-ol [31]. Phytochemicals analysis of Hamelia patens with different extract [32] is given in ▶Tables 2 and ▶3.…”

GC-MS Analysis of Spondias mombin (Linn) Methanol Leaf Extract

“…Medicinal plant in treatment of diseases is as old as man. Many developing countries of the world look upon medicinal plants as possible substitute to synthetic drug because natural sources of herbal medicines are more protective, safe, biodegradable, fewer side effects than artificial and synthetic remedies because the photochemical in the plants extract target the biochemical pathways [1]. The entire dependence of man on plants and plant products directly for his basic needs make plants vital to his survival and the basis of his continued existence.…”