Evaluation and insights into chitosan antimicrobial activity against anaerobic oral pathogens

Antimicrobial Effect of Chitosan against Periodontal Pathogens Biofilms

“…Transparent 25 ml plastic tubes were inoculated at 1% (v/v) in WCB supplemented with 5% (m/v) sucrose. Chitosan was added at sub-MIC (at ½ and ¼ of the MIC) concentrations selected according to the MIC results determined by Costa, et al [9] (Table 1). Each tube was incubated in horizontal position at 37°C for 72h in a rectangular anaerobiosisjar (AnaeroPack, Mitsubishi gas chemical company, Japan).…”

“…The differences were evaluated using one way ANOVA test HMW (mg/mL) LMW (mg/mL) Table 1: Chitosan sub-MIC tested for each bacterium in mg/mL. Values based on those previously described by Costa et al [9].…”

“…Considering the known properties of chitosan there are a few possible explanations for the results obtained. As several authors have reported [9,12,24], HMW chitosan activity is focused upon the bacterial membrane and as such it is possible that this disruption may interfere with C. violaceumviolacein production metabolism. When looking into LMW chitosan the same authors described that it was capable of entering the bacterial cell where it interfered with cellular machinery, as such it is possible that LMW chitosan is interfering in the violacein metabolic synthesis, possibly through interaction with violacein metabolic precursor prodeoxyviolacein, which synthesis involves a four-electron oxidative step [25].…”

A quitosana como biomaterial odontológico: estado da arte

“…Nestes estudos (Costa et al, 2012;Elsaka e Elnaghy, 2012;Íkinci et al, 2002), a quitosana foi considerada como tendo atividade bactericida ou bacteriostática, frequentemente sem distinção entre estas atividades. Dados recentes da literatura caraterizam a quitosana como sendo mais bacteriostática do que bactericida (Coma et al, 2002), apesar do mecanismo exato não ser ainda exatamente conhecido e a ação antibacteriana estar dependente da interação de diferentes fatores (Raafat et al, 2008).…”

Chitosan in different concentrations added to a two-step etch-and-rinse adhesive system: influence on bond strength to dentin

“…It has the characteristics of biocompatibility and biodegradability, and is used as a biomaterial in the area of health [6,10,11]. Its activity is reported to be antimicrobial against bacteria; fungi and viruses; and anti-inflammatory [10][11][12][13], in addition to being capable of inhibiting the growth of Porphyromonas gingivalis (Pg) and Aggregatibacter actinomycetemcomitans [14,15]. Thus it is a very versatile polymer that is easily modifiable to improve the use of its biological and physical-chemical properties [10].…”