Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring

Kang, Yeong-Lim; Jeong, Ji-Yun; Choi, Hwi-Yeon; Zhang, Yanhong; Liu, Yumei; Lee, Ho-Jong; Choi, Jong-Chul; Lee, SoHyun; Lee, Beom-Joo; Lee, Sang‐Won; Lee, Joong‐Bok; Cho, Ki-hyun; Park, Seung Yong

doi:10.1186/s12917-018-1686-7

Cited by 8 publications

(4 citation statements)

References 17 publications

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“…Among these tests, SPCE is widely used for primary screening to determine SP Abs in field samples due to the time and labor consumption of VNTs as well as the lack of antigen stability of LPBE. For the accurate diagnosis of FMD, SPCE for FMD is currently also needed to improve sensitivity and specificity [28]. Many efforts have been made to develop mAbs against SP Abs for FMDV type A [29][30][31], but it has been required to develop a FMDV type A SPCE with higher sensitivity and specificity until now.…”

Section: Discussionmentioning

confidence: 99%

Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis

et al. 2019

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Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease affecting cloven-hoofed livestock worldwide. FMD virus (FMDV) type A is one of the most common causes of FMD outbreaks among the seven FMDV serotypes, and its serological diagnosis is therefore important to confirm FMDV type A infection and to determine FMD vaccine efficacy. Here, we generated monoclonal antibodies (mAbs) specific to FMDV type A via hybridoma systems using an inactivated FMDV type A (A22/Iraq/1964) and found 4 monoclones (#29, #106, #108, and #109) with high binding reactivity to FMDV type A among 594 primary clones. In particular, the #106 mAb had a higher binding reactivity to the inactivated FMDV type A than the other mAbs and a commercial mAb. Moreover, the #106 mAb showed no cross-reactivity to inactivated FMDV type South African territories 1, 2, and 3, and low reactivity to inactivated FMDV type O (O1 Manisa). Importantly, the solid-phase competitive ELISA (SPCE) using horseradish peroxidase (HRP)-conjugated #106 mAb detected FMDV type A-specific Abs in sera from FMD type A-vaccinated cattle more effectively than a commercial SPCE. These results suggest that the newly developed FMDV type A-specific mAb might be useful for diagnostic approaches for detecting Abs against FMDV type A.

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Section: Discussionmentioning

confidence: 99%

Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis

et al. 2019

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“…Solid-phase competition ELISA kits for detection of specific SPs of FMDV of different serotypes were commercially available. Nevertheless, one recent study reported that the sensitivity of the SPC-ELISA kit for specific detection of FMDV serotype O was lower, and it produced more false-positive results as compared to LPB-ELISA and VNT ( 78 ).…”

Section: Serological Methodsmentioning

confidence: 99%

Advances in the Diagnosis of Foot-and-Mouth Disease

et al. 2020

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Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous economic losses to livestock industry and subsequently lead to serious socioeconomic damages due to the immediate imposition of trade embargo. Rapid and accurate diagnoses are imperative to control this infectious virus. In the current review, enzyme-linked immunosorbent assay (ELISA)-based methods used in FMD diagnosis are extensively reviewed, particularly the sandwich, liquid-phase blocking, and solid-phase competition ELISA. The differentiation of infected animals from vaccinated animals using ELISA-based methods is also highlighted, in which the role of 3ABC polyprotein as a marker is reviewed intensively. Recently, more studies are focusing on the molecular diagnostic methods, which detect the viral nucleic acids based on reverse transcription-polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (RT-LAMP). These methods are generally more sensitive because of their ability to amplify a minute amount of the viral nucleic acids. In this digital era, the RT-PCR and RT-LAMP are progressing toward the mobile versions, aiming for on-site FMDV diagnosis. Apart from RT-PCR and RT-LAMP, another diagnostic assay specifically designed for on-site diagnosis is the lateral flow immunochromatographic test strips. These test strips have some distinct advantages over other diagnostic methods, whereby the assay often does not require the aid of an external device, which greatly lowers the cost per test. In addition, the on-site diagnostic test can be easily performed by untrained personnel including farmers, and the results can be obtained in a few minutes. Lastly, the use of FMDV diagnostic assays for progressive control of the disease is also discussed critically.

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“…The presence of serotype specific anti-SP antibodies in serum samples was tested by using the FMD SPC ELISA kits (IZSLER, Biotech Lab, Italy). Selected neutralizing anti-FMDV monoclonal antibodies were used in solid phase competitive ELISA, specific for FMDV serotype Asia 1, O, and A to measure antibodies against these serotypes (Kang et al, 2018). Optical density of the test wells compared to the control wells was read by a microplate photometer (Thermo, 590741) and interpreted as negative or positive based on the percentage inhibition (PI).…”

Section: Solid-phase Competitive Elisa (Spc Elisa)mentioning

confidence: 99%

Circulation of Predominantly Prevalent Serotypes of Foot and Mouth Disease Virus in Bovine Animals in Bahawalpur, Pakistan

Abbas¹,

Rahman²,

Abubakar³

et al. 2023

SJA

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Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring

Cited by 8 publications

References 17 publications

Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis

Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis

Advances in the Diagnosis of Foot-and-Mouth Disease

Circulation of Predominantly Prevalent Serotypes of Foot and Mouth Disease Virus in Bovine Animals in Bahawalpur, Pakistan

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