Evaluation, comparison of different solvent extraction, cell disruption methods and hydrothermal liquefaction of Oedogonium macroalgae for biofuel production

Abstract

Graphical abstract

Macroalgae—A Sustainable Source of Chemical Compounds with Biological Activities

“…Several studies were conducted on different glycolipids from seaweed (e.g., monoglycosyl diacylglycerol subfraction from Fucus distichus; monogalactosyl diacylglycerols from Sargassum horneri; sulfoglycolipids from Porphyra crispata) , showing their anti-inflammatory and antiproliferative effects, respectively [ 27 , 28 , 29 ]. Regarding lipid extraction, Ramola et al [ 30 ] and Margareta et al [ 31 ] found that the most efficient solvent was a mixture of chloroform: methanol (2:1) with an efficiency of 14% compared to hexane (2:1) with 12.5% efficiency.…”

Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194

“…However, rating the methods is difficult, as reported results of different laboratories may not be comparable. Although the importance of cell disruption for lipid extraction efficiency has been recognized (Rakesh et al, 2015;Ramola et al, 2019), cell disruption is usually not determined and therefore, thresholds for effective extractions have not been communicated.…”

Production, purification, and process optimization of intracellular pigment from novel psychrotolerant Paenibacillus sp. BPW19

“…The cell culture was divided into four batches and was primarily subjected to four different types of cell lysis treatment, respectively. The treatment methods include sonication for 30 min; boiling at 100 °C for 15 min; boiling at 100 °C for 10 min followed by sonication for 20 min; alkali lysis using 1 M NaOH for 10 min and adding 1 M HCl to cease the lysis treatment post the incubation period [ 44 ].…”