Evaluation of 16S rRNA broad range PCR assay for microbial detection in serum specimens in sepsis patients

Mishra, Deepika; Satpathy, Gita; Wig, Naveet; Fazal, Farhan; Ahmed, Nishat Hussain; Panda, Subrat Kumar

doi:10.1016/j.jiph.2020.01.007

Cited by 23 publications

(14 citation statements)

References 15 publications

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“…Candidatus Chryseobacterium massiliae was positively correlated with ACTH and CORT, and was negatively correlated with IL-10 and GABA. Enhydrobacter aerosaccus was positively correlated with CORT and T3; Enhydrobacter aerosaccus has been reported to be detected from a patient with haemophagocytic lymphohistiocytosis who was using corticosteroids ( 29 ). Corynebacterium falsenii was positively correlated with T3 and CORT, and was recognized as a primary pathogen ( 30 ).…”

Section: Resultsmentioning

confidence: 99%

Multi-Omics Analysis Reveals the Systematic Relationship Between Oral Homeostasis and Chronic Sleep Deprivation in Rats

Chen

Yao

et al. 2022

Front. Immunol.

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Sleep disorders were associated with oral health. Inflammation has especially been thought to be a key factor in linking oral diseases and sleep deficiency. However, how chronic sleep deprivation (CSD) affects oral homeostasis, particularly oral inflammation and oral microbiota, is still unknown. This study aimed to uncover the systematic relationship between oral homeostasis and CSD in rats. The metabolomics in serum, proteomics in the tongue tissues, and microbiome analysis in the oral cavity in CSD rats were performed. Multi-omics data integration analysis was performed to uncover the systematic relationship between oral homeostasis and CSD through the weighted correlation network analysis. We found that CSD could lead to oral inflammation in rats. CSD significantly increased systemic inflammation by enhancing the serum levels of IL-1β, IL-6 and inhibiting the serum level of IL-10. Serum levels of adrenocorticotropin hormone, corticosterone, and triiodothyronine were increased in CSD rats, and the steroid hormone biosynthesis pathway was also found to be involved in the perturbation resulting from CSD, together suggesting the activation of the hypothalamic-pituitary-adrenocortical and hypothalamic‐pituitary‐thyroid axis. CSD led to changes of oral microbiota composition, and g_Acinetobacter, Candidatus Chryseobacterium massiliae, and g_Moraxella were significantly correlated with multiple proteins in bacterial invasion of epithelial cells pathway, which may partially responsible for oral inflammation resulting from CSD. The changes of proteomic profiling expression caused by CSD in tongue tissues were mainly enriched in neurodegenerative diseases pathways and immune/inflammation-related pathways. Multi-omics analysis indicated that the inflammatory response-related modules were significantly correlated with the neurodegenerative disease-related module suggesting a possible link between neurodegenerative diseases and oral inflammation. Together, CSD induced oral inflammation and subtle changes on oral microbiota. Our study is helpful to further understand the role that oral homeostasis plays in the process by which CSD affects human health and disease.

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Section: Resultsmentioning

confidence: 99%

Multi-Omics Analysis Reveals the Systematic Relationship Between Oral Homeostasis and Chronic Sleep Deprivation in Rats

Chen

Yao

et al. 2022

Front. Immunol.

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“…Bradyrhizobium enterica were found in patients with colitis [36]. Enhydrobacter aerosaccus can be detected from a patient with Hemophagocytic lymphohistiocytosis (HLH) with concomitant corticosteroid use [37]. This is particularly of interest due to the growing evidence of HLH syndrome in severe COVID-19 patients [38].…”

Section: Discussionmentioning

confidence: 99%

Endotoxemia and circulating bacteriome in severe COVID-19 patients

Sirivongrangson

Kulvichit

Payungporn

et al. 2020

ICMx

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Background When severe, COVID-19 shares many clinical features with bacterial sepsis. Yet, secondary bacterial infection is uncommon. However, as epithelium is injured and barrier function is lost, bacterial products entering the circulation might contribute to the pathophysiology of COVID-19. Methods We studied 19 adults, severely ill patients with COVID-19 infection, who were admitted to King Chulalongkorn Memorial Hospital, Bangkok, Thailand, between 13th March and 17th April 2020. Blood samples on days 1, 3, and 7 of enrollment were analyzed for endotoxin activity assay (EAA), (1 → 3)-β-d-glucan (BG), and 16S rRNA gene sequencing to determine the circulating bacteriome. Results Of the 19 patients, 13 were in intensive care and 10 patients received mechanical ventilation. We found 8 patients with high EAA (≥ 0.6) and about half of the patients had high serum BG levels which tended to be higher in later in the illness. Although only 1 patient had a positive blood culture, 18 of 19 patients were positive for 16S rRNA gene amplification. Proteobacteria was the most abundant phylum. The diversity of bacterial genera was decreased overtime. Conclusions Bacterial DNA and toxins were discovered in virtually all severely ill COVID-19 pneumonia patients. This raises a previously unrecognized concern for significant contribution of bacterial products in the pathogenesis of this disease.

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“…Bradyrhizobium enterica were found in patients with colitis [34]. Enhydrobacter aerosaccus can be detected from a patient with Hemophagocytic lymphohistiocytosis (HLH) with concomitant corticosteroid use [35]. This is particularly of interest due to the growing evidence of HLH syndrome in severe COVID-19 patients [36].…”

Section: Discussionmentioning

confidence: 99%

Endotoxemia and circulating bacteriome in severe COVID-19 patients

Sirivongrangson

Kulvichit

Payungporn

et al. 2020

Preprint

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Background: When severe, COVID-19 shares many clinical features with bacterial sepsis. Yet, secondary bacterial infection is uncommon. However, as epithelium are injured and barrier function is lost, bacterial products entering the circulation might contribute to the pathophysiology of COVID-19. Methods: We studied 19 adults, severely ill patients with COVID-19 infection, who were admitted to King Chulalongkorn Memorial Hospital, Bangkok, Thailand, between 13th March and 17th April 2020. Blood samples on day 1, 3, and 7 of enrollment were analyzed for endotoxin activity assay (EAA), Beta-D-Glucan (BG), and 16S rRNA gene sequencing to determine the circulating bacteriome. Findings: Of the 19 patients, 14 were in intensive care and 10 patients received mechanical ventilation. We found 8 patients with high EAA (≥ 0.6) and about half of the patients had high serum BG levels which tended to be higher in later in the illness. Although only 1 patient had a positive blood culture, 18 of 19 patients were positive for 16S rRNA gene amplification. Proteobacteria was the most abundant phylum. The diversity of bacterial genera was decreased overtime. Interpretation: Bacterial DNA and toxins were discovered in virtual all severely ill COVID-19 pneumonia patients. This raises a previously unrecognized concern for significant contribution of bacterial products in the pathogenesis of this disease.

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Evaluation of 16S rRNA broad range PCR assay for microbial detection in serum specimens in sepsis patients

Cited by 23 publications

References 15 publications

Multi-Omics Analysis Reveals the Systematic Relationship Between Oral Homeostasis and Chronic Sleep Deprivation in Rats

Multi-Omics Analysis Reveals the Systematic Relationship Between Oral Homeostasis and Chronic Sleep Deprivation in Rats

Endotoxemia and circulating bacteriome in severe COVID-19 patients

Endotoxemia and circulating bacteriome in severe COVID-19 patients

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