Evaluation of 1p and 19q deletion by chromogenic in situ hybridization (CISH) in paraffin-embedded central nervous system oligodendroglial tumors (CNSOT).

Bella, Santiago; Theaux, Ricardo; Papalini, Francisco; Suárez, Julio Cesar Pérez; Guidi, Anthony J.; Armando, Lucas; Sambuelli, Rubén; Gómez, Sánchez; Bernhardt, Christian; Lerda, Daniel

doi:10.1200/jco.2010.28.15_suppl.e12557

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“…Using a light microscope instead of a fluorescence microscope and the ability to easily observe the morphological characteristics of the fund will be seen as advantages over current FISH assays. Based on data from different studies [6,17,18], our experience using the technique of CISH [19][20][21][22][23] and the results of this study, the dual-color DAKO CISH assay appears to be sensitive and specific, and because of his familiarity with immunohistochemical analysis, which may have the potential to be the most used in pathology laboratories in the future.…”

Section: Discussionmentioning

confidence: 94%

Evaluation of Myc Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic in-Situ Hybridization (Dual CISH) Assay

Lerda¹,

Cabrera²,

Flores³

et al. 2013

J Interdiscipl Histopathol

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Objetive: The overall purpose of the study was to demonstrate applicability of the Dako dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, Glostrup) with respect to fluorescence in situ hybridization (FISH) probes MYC-C. Methods: MYC gene amplification by FISH and Dako dual-color CISH Results: The study showed that the dual-color CISH assay can convert Texas red and fluorescein isothiocyanate (FITC) signals into chromogenic signals. The dual –color CISH assay was performed on 40 cases of prostate cancer. Amplification was identified in 12 of 40 (30%) tumors. No amplification was seen in 28 of 40 (70%) tumors. FISH data were available in total of 40 tumors. All tumors showed concordant results between dual-color CISH and FISH for classifying a tumor as MYC amplified or not amplified. Conclusions: We conclude that dual-color Dako CISH assay is an accurate method for determining MYC gene amplification with added advantages that make it a more practically useful method. [J Interdiscipl Histopathol 2013; 1(2.000): 81-84

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