Evaluation of a commercially available ELISA assay for detection of Giardia lamblia in fecal specimens

Aldeen, William E.; Hale, De Von; Robison, Alexander J.; Carroll, Karen C.

doi:10.1016/0732-8893(94)00142-j

Cited by 38 publications

(42 citation statements)

References 6 publications

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“…In a recent study, ELISA's sensitivity and specificity compared to microscopy was 72.9% and 100%, respectively (22). In several other studies conducted with different immunoassay kits, excellent specificity rates within the range of 91.5%-100% were reported and the specificity values for all EIAs exceeded 99% (23,24). In a study conducted with the same commercial EIA kit used in this study (Giardia CELISA, Cellabs), the sensitivity (63%) was found to be in concordance with our findings (13).…”

Section: Discussionmentioning

confidence: 90%

Comparison of microscopy, ELISA, and real-time PCR for detectionof Giardia intestinalis in human stool specimens

Beyhan¹,

Cengiz²

2017

Turk J Med Sci

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Background/aim: This study included patients who had digestive system complaints between August 2015 and October 2015. The research was designed to compare conventional microscopy with an antigen detection ELISA kit and the TaqMan-based real-time PCR (RT-PCR) technique for detection of Giardia intestinalis in human stool specimens.Materials and methods: Samples were concentrated by formalin-ether sedimentation technique and microscopic examinations were carried out on wet mount slides. A commercially available ELISA kit (Giardia CELISA, Cellabs, Brookvale, Australia) was used for immunoassay. DNA was extracted from fecal samples of about 200 mg using the QIAamp Fast DNA Stool Mini Kit (QIAGEN, Hilden, Germany) and the LightCycler Nano system (Roche Diagnostics, Mannheim, Germany) was used for the TaqMan-based RT-PCR assay.Results: A total of 94 stool samples, 38 of them diagnosed positive (40.4%) and 56 of them diagnosed negative by microscopy, were selected for evaluation by antigen detection and molecular assays. The prevalence of G. intestinalis infection was found as 46.8% (n: 44) and 79.8% (n: 75) by ELISA and RT-PCR, respectively. RT-PCR revealed by far the highest positivity rate compared to the other two methods. The difference between these methods was found to be statistically significant (P < 0.05). In comparison to PCR, the sensitivity and specificity of microscopy and ELISA were 50.7% and 100% and 53.3% and 79%, respectively. Conclusion:RT-PCR seems to be much more sensitive and beneficial for rapid and accurate diagnosis of G. intestinalis in human stools.

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Section: Discussionmentioning

confidence: 90%

Comparison of microscopy, ELISA, and real-time PCR for detectionof Giardia intestinalis in human stool specimens

Beyhan¹,

Cengiz²

2017

Turk J Med Sci

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“…It is stable and is secreted in large amounts by encysting trophozoites (Rosoff & Stibbs, 1986b). According to Aldeen et al (1998), the sensitivity and specificity of the assay is 100% in fresh feces. The immunological test performed was rapid and simple, although expensive.…”

Section: Carvalho Gonçalves Et Al In Trans Royal Society Of Tropicamentioning

confidence: 99%

“…Several studies have found ELISA to be a tool with very high sensitivity and specificity for G. duodenalis coproantigen detection Ganoff et al, 1989;ROSOFF et al, 1989;ADDISS et al, 1991;Scheffler & Van Etta, 1994;Aldeen et al, 1998;Garcia et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Detection of Giardia duodenalis antigen in coprolites using a commercially available enzyme-linked immunosorbent assay

Gonçalves

Araújo

Duarte

et al. 2002

Transactions of the Royal Society of Tropical Medicine and Hygi

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“…16,17 have raised awareness of the human health risks and motivated the development of new diagnostic tests for people. Although extensively validated in people, [18][19][20][21] few studies document the performance characteristics (eg, sensitivity, specificity, ease of use) of these tests in cats. 22 A recent study 22 examined several commercially available immunoassays for the diagnosis of Cryptosporidium spp.…”

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confidence: 99%

Comparison of Direct Immunofluorescence, Immunoassays, and Fecal Flotation for Detection of Cryptosporidium spp. and Giardia spp. in Naturally Exposed Cats in 4 Northern California Animal Shelters

Mekaru

Marks

Felley

et al. 2007

Veterinary Internal Medicne

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Background: Giardia spp. and Cryptosporidium spp. are common intestinal protozoan parasites in domestic cats. Few studies have critically evaluated the performance characteristics of commercially available immunoassays for detection of these organisms in the cat.Hypothesis: Human-based immunoassays are suboptimal for the detection of Giardia spp. and Cryptosporidium spp. in cats. Animals: Three-hundred-and-forty-four cats with diarrheic and nondiarrheic fecal specimens at 4 northern California animal shelters.Methods: A fecal specimen was collected from each cat in a case-controlled fashion. Fecal specimens were tested for Giardia spp. and Cryptosporidium spp. by using centrifugation flotation and 5 commercially available immunoassays (SNAP Giardia, ProSpecT Giardia Microplate Assay, ProSpecT Cryptosporidium Microplate Assay, ImmunoCard STAT! Cryptosporidium/ Giardia Rapid Assay, and Xpect Giardia/Cryptosporidium). Results were compared with a reference standard, the MeriFluor direct immunofluorescence assay.Results: Overall prevalences of Giardia spp. and Cryptosporidium spp. were 9.8 and 4.7%, respectively. The ProSpecT Microplate Assay had the highest sensitivities and specificities for Giardia spp. (91.2 and 99.4%) and Cryptosporidum spp. (71.4 and 96.7%), respectively. The SNAP Giardia antigen assay was easier to use and equally sensitive (85.3%) and specific (100%) to fecal flotation.Conclusions and Clinical Importance: Caution should be exercised when using human-based immunoassays for the diagnosis of Giardia and Cryptosporidium spp. in cats. Fecal flotation remains a useful method for detection of Giardia spp., can be used to detect other parasites, and has a sensitivity of 97.8% for detection of Giardia spp. when combined with the SNAP Giardia immunoassay.

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Evaluation of a commercially available ELISA assay for detection of Giardia lamblia in fecal specimens

Cited by 38 publications

References 6 publications

Comparison of microscopy, ELISA, and real-time PCR for detectionof Giardia intestinalis in human stool specimens

Comparison of microscopy, ELISA, and real-time PCR for detectionof Giardia intestinalis in human stool specimens

Detection of Giardia duodenalis antigen in coprolites using a commercially available enzyme-linked immunosorbent assay

Comparison of Direct Immunofluorescence, Immunoassays, and Fecal Flotation for Detection of Cryptosporidium spp. and Giardia spp. in Naturally Exposed Cats in 4 Northern California Animal Shelters

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