Evaluation of a concurrent multicomponent collection system for the collection and storage of WBC‐reduced RBC apheresis concentrates

Moog, Rainer; Franck, Valentine; Pierce, Jennifer A.; Müller, Norbert

doi:10.1046/j.1537-2995.2001.41091159.x

Cited by 26 publications

(20 citation statements)

References 18 publications

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“…A possible explanation for the increased haemolysis could be the shear stress due to the multiple centrifugation steps. During MNC separation, the RBCs were exposed to low centrifugal forces of 136 g and 930 g compared to 1270 g used during the processing of homologous PRBCs from whole blood but the centrifugation time of approximately 62 min was about six times longer than during the conventional process of whole blood derived PRBCs (P < 0AE05), confirming previous results that showed the impact of mechanical forces on the extent of red cell damage [19].Potassium, lactate and pH values, in contrast, remained within the ranges of freshly collected RBCs during the whole production process [11,20]. To maintain a physiological ion gradient in RBCs, the function of the Na+ ⁄ K+ pump, which is powered by intracellular purines, is essential.…”

supporting

confidence: 81%

“…Here, we included 19 patients with myocardial infarction enclosed in the MYSTAR study, who received enriched CD34 + cells in a volume of approximately 4 ml intramyocardially [16,18]. Despite depletion of haemolytic supernatant from the remaining RBC fraction after BM procession, the recovered RBCs showed higher LDH and fHb levels as compared with unprocessed BM or homologous packed RBC concentrates (PRBCs) at the end of shelf life at day 42 [11,19,20]. In two units of recovered RBCs, haemolysis was even above the threshold of 0AE8% [14].…”

Section: Discussionmentioning

confidence: 99%

“…Potassium, lactate and pH values, in contrast, remained within the ranges of freshly collected RBCs during the whole production process [11,20]. To maintain a physiological ion gradient in RBCs, the function of the Na+ ⁄ K+ pump, which is powered by intracellular purines, is essential.…”

Section: Discussionmentioning

confidence: 99%

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Red blood units collected from bone marrow harvests after mononuclear cell selection qualify for autologous use

et al. 2010

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Background After large volume bone marrow (BM) harvest, donors and patients can develop severe anaemia, because collected BM can contain up to 20% of their red cell mass. In a prospective analysis, we investigated the feasibility to recover red blood cells (RBCs) from the harvested BM and investigated whether these RBC units meet the quality requirements of the European Council.Patients and Methods From 19 patients (median age 51 yrs, range 31-77) with acute myocardial infarction, who participated in the MYSTAR study, a median volume of 1299 ml (range, 700-1870 ml) BM was collected. During BM processing, mononuclear cells (MNC) were separated using the Cobe SpectraÔ apheresis system and the residual RBCs were collected in a separate bag. The quality of the collected RBCs was assessed by measuring LDH, free haemoglobin, potassium and lactate. Haemolysis was calculated and the intracellular concentration of ATP, ADP, AMP was determined by HPLC.Results RBC units recovered from BM after MNC separation had a mean volume of 312 ± 95 ml with a haematocrit of 47 ± 8AE9%, a haemoglobin content of 51 ± 15 g per unit, a haemolysis of 0AE15 ± 0AE005%, a pH of 6AE8 ± 0AE007 and an intracellular ATP concentration of 135 pmol ⁄ 10 6 RBC ± 41, which is comparable with freshly collected packed red blood cells (PRBCs).Conclusion RBCs, collected from bone marrow harvests, can be used for autologous blood support to minimize allogeneic blood transfusions in donors and patients after large volume BM donation.Key words: blood sparing measurements, bone marrow harvest, bone marrow processing, salvage of red blood cells. IntroductionLarge volume bone marrow (BM) harvest is an alternative to G-CSF-induced peripheral blood stem cell collection in patients or donors who are poor mobilizers, in the paediatric setting or in the regenerative medicine, when patients with myocardial infarction are intended to receive stem cells intracardially [1][2][3][4]. Donors and patients are at risk to develop a severe anaemia because collected BM can contain up to 20% of their red cell mass. To reduce the risk of anaemia after BM donation, at least one unit of autologous blood should be collected prior to the harvest procedure [5]. Alternatively, certain clinicians place the donors on supplemental iron plus rhEPO, transfuse their donors with allogeneic RBC units or tolerate anaemia when not severe [6]. Previous studies have shown that a recovery of approximately 90% of the harvested BM-RBCs decreased the homologous transfusion requirement of healthy BM donors significantly [7]. When paediatric BM donors were investigated and BM-RBCs were separated by density gradient centrifugation using Ficoll-Hypaque, similar results were In a prospective analysis, we assessed the extent of cell lesions occurring during manipulation by measuring the release of lactate dehydrogenase (LDH), free haemoglobin (fHb), potassium (K+) and lactate at each manipulation step. Special attention was paid to intracellular purine content by measuring ATP, ADP, AMP, as a surrogate mar...

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confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Red blood units collected from bone marrow harvests after mononuclear cell selection qualify for autologous use

et al. 2010

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“…New technical developments allow for the collection of RBCs by apheresis (ARBCs), which is a fundamental evolution in transfusion technology and probably offers the potential for improved product consistency and quality. [1][2][3][4][5][6] From the donor's point of view, automated RBC collection is less often associated with vasovagal reactions, most likely due to better fluid balance. 7,8 Storage of RBCs suspended in a plasma-free additive solution such as saline-adenine-glucose-mannitol (SAGM) is currently limited to 42 days during which the 24-hour posttransfusion recovery decreases gradually as the so-called "storage" and "collection injuries" increase.…”

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confidence: 99%

In vitro quality of red blood cells (RBCs) collected by multicomponent apheresis compared to manually collected RBCs during 49 days of storage

2007

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“…Briefly, the platelets are extracted using an apheresis machine (TEC.COM, Fresenius Kabi) which can lead to a reproducible platelet concentration of 850 000 units/microlitre, a value approximatively 4 times as high as in the whole blood [11]. 6 millilitres of this PRP were mixed with 300 microlitres of calcium chloride to activate the platelets.…”

Section: Are There Biologic Factors Of Good Prognosis After a Platelementioning

confidence: 99%

Are There Biologic Factors of Good Prognosis after a Platelet Rich Plasma (PRP) Injection for Tendinopathies ?

Libertiaux

Sancerne²,

Goff

et al. 2016

JESO

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Open Access Short communicationof extrinsinc factors include unadapted material, bad training planification, or usage of some drugs (quinolones, corticoids,…) [4]. Some patients are refractory to the traditional treatments (including physiotherapy, shockwaves, deep transverse massage, etc.) and thus need another option. Less invasive than surgery, Platelet-Rich Plasma (PRP) injection has become more and more popular over the last decades, particularly in sports medicine [5][6][7]. In this paper, in addition of presenting the data asserting the effectiveness of injections of reproducible PRP collected from an apheresis machine on different tendinopathies, we introduce another original contribution : the investigation of the possible correlation between several biomarkers and healing evolution to determine the factors of good prognosis, if any. Material and MethodsForty-eight patients (mean age = 49 y.o +/-10 y.o, min = 26 y.o, max = 77 y.o. ; 15 women and 33 men) afflicted with different pathologies participated in this study after they gave their informed consent (lateral epicondylitis (n=20), medial epicondylitis (n=3), tendinopathy of the gluteus medius (n=1), patellar tendinopathy (n=6), calcaneal tendinopathy (n=14), and plantar fasciitis (n=4)). To be recruited, the patients have had to suffer from a chronic tendinopathy for more than 3 months and be refractory to the following classical treatments : non steroidal anti inflammatory drugs, electrotherapy, deep transverse massage, eccentric revalidation and shockwaves therapy. A clinical examination was carried out to confirm the diagnosis and to exclude any other pathology, including metabolic disease. After this first step, a blood sample was drawn and analyzed to quantify the presence of various metabolic biomarkers (glycemia, thyroid hormons, cholesterolemia, uricemia) which are considered to be risk factors of tendinopathies. The initial pain assessment was made using a Visual Analogic Scale (VAS) of pain and a pressure algometer (Commander Algometer, Tech Medical) prior to the PRP collection. The PRP collection and injection protocol used has already been described in previous AbstractBackground : The aims of this study were to evaluate the effect of a single PRP injection followed by a standardized reeducation protocol among patients suffering from different tendinopathies, but otherwise healthy, and to determine the biomarkers that constitute good prognosis factors, if any.

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Evaluation of a concurrent multicomponent collection system for the collection and storage of WBC‐reduced RBC apheresis concentrates

Abstract: The concurrent multicomponent collection system (Amicus) can reliably collect a standardized RBC unit of good quality. In vitro testing of the RBCs collected and stored for 42 days met the Council of Europe criteria for transfusion.

Cited by 26 publications

References 18 publications

Red blood units collected from bone marrow harvests after mononuclear cell selection qualify for autologous use

Red blood units collected from bone marrow harvests after mononuclear cell selection qualify for autologous use

In vitro quality of red blood cells (RBCs) collected by multicomponent apheresis compared to manually collected RBCs during 49 days of storage

Are There Biologic Factors of Good Prognosis after a Platelet Rich Plasma (PRP) Injection for Tendinopathies ?

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