Evaluation of a lateral flow–based technology card for blood typing using a simplified protocol in a model of extreme blood sampling conditions

Clavier, B.; Pouget, Thomas; Sailliol, A.

doi:10.1111/trf.14420

Cited by 1 publication

(1 citation statement)

References 12 publications

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“…The MDmulticard Basic Extended Phenotype might become an equal or even superior alternative for such purposes. Moreover, the MDmulticard system could not only be adequate for blood typing in emergency situations [4,8,9,10] and extreme environment conditions [22], but could also be more cost-effective, as it limits the number of genotyping investigations necessary (and therefore the costs) to provide antigen-matched blood, especially for patients with autoantibodies or sickle cell disease. However, this should be confirmed by studies specifically aimed to cost and time analyses [23].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the New Lateral Flow Card MDmulticard® Basic Extended Phenotype in Routine Clinical Practice

Mayer

Müller

Candela-García

et al. 2018

Transfus Med Hemother

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Background: Transfusion emergencies and critical situations require specifically designed devices to simplify and optimize the standard procedures. In addition, matching antigens over and above ABO-Rh-K would be beneficial. Methods: Routine blood samples were collected in four immunohematology centers and tested with the new MDmulticard Basic Extended Phenotype for the simultaneous detection of the Duffy, Kidd, and Ss antigens, according to the principle of the lateral flow. Results were compared with those obtained using routine serology methods. Discrepancies were analyzed by molecular techniques/genotyping. Results: 310 samples were tested (167 donors; 75 patients; 28 subjects with positive direct antiglobulin test (DAT); 15 newborns; 25 previously transfused patients). The 285 samples with non-mixed-field reaction yielded 1,710 antigen results with 8 discrepancies (0.47%) six of which in DAT-positive subjects: three false-positive (Fy^a) for MDmulticard, and two false-positive (Fy^a) plus three false-negative (Fy^b) for the reference methods (MDmulticard PPA for donors/patients/newborns: 99.82%; negative percent agreement: 100%; sensitivity: 100%; specificity: 99.39%, positive predictive value: 99.75%; negative predictive value: 100%). The MDmulticard detected mixed-field in 15 antigen reactions from 13 transfused patients, undetected by the comparative method, with the opposite result in 8 antigens (5 patients). Conclusion: The MDmulticard Basic Extended Phenotype met the criteria prescribed for the testing of donor, patient, DAT-positive, and newborn samples in transfusion laboratory routine.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the New Lateral Flow Card MDmulticard® Basic Extended Phenotype in Routine Clinical Practice

Mayer

Müller

Candela-García

et al. 2018

Transfus Med Hemother

View full text Add to dashboard Cite

show abstract

Evaluation of a lateral flow–based technology card for blood typing using a simplified protocol in a model of extreme blood sampling conditions

Abstract: The simplified protocol for MDmulticard use could be considered a reliable method for blood typing in extreme environment or emergency situations, worsened by red blood cell dilution or concentration.

Cited by 1 publication

References 12 publications

Evaluation of the New Lateral Flow Card MDmulticard® Basic Extended Phenotype in Routine Clinical Practice

Evaluation of the New Lateral Flow Card MDmulticard® Basic Extended Phenotype in Routine Clinical Practice

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