Evaluation of a latex test for rotavirus detection

Brandt, Carl D.; Arndt, C W; Evans, G.; Kim, H W; Stallings, E P; Rodriguez, Wm J; Parrott, Robt H

doi:10.1128/jcm.25.9.1800-1802.1987

Cited by 23 publications

(7 citation statements)

References 16 publications

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“…However, 12 specimens positive by the Enzygnost test only and 3 specimens positive by the Rotalex test only could not be confirmed positive by EM [38]. In the same way Slidex Rota-Kit were found to be 82% sensitive and 100% specific [39].…”

Section: Evaluation Of Rotavirus Diagnostic Techniquesmentioning

confidence: 76%

Evaluation of Techniques for the Diagnosis of Rotavirus Gastroenteritis in Paediatric Patients

R.K¹,

Oke²,

Anjorin³

et al. 2015

Genes Review

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Rotavirus is the major etiology of acute gastroenteritis in infants and young children worldwide. It has been estimated that about 39% of childhood diarrhoea hospitalizations are caused by the virus and nearly half a million children die from the infections each year especially in developing countries on the Asian subcontinent, Africa, and Latin America where health care facilities are in short supply. Stool specimens collected from the first to fourth days of illness are optimal for detection of the infection. Diagnosis was originally performed using electron microscopy, which is still occasionally used in centers where it is available. Routine diagnosis is however, now performed by antigen detection on feces using commercially available, simple, rapid immunochromatographic dipstick style kits which have superseded the earlier latex agglutination and enzyme immunoassay methods whose usefulness in diagnosis is still relevant. Reverse transcription polymerase chain reaction of feces is also available in some reference and research centers, and is particularly useful for identification of outbreaks due to serogroups other than group A. Similarly viral culture and serology are also available but do not play much roles in diagnosis of acute disease. Rotavirus infection is indeed a public health problem and early, rapid, accurate and reliable diagnostic techniques are essential for effective patient management and infection control.

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Section: Evaluation Of Rotavirus Diagnostic Techniquesmentioning

confidence: 76%

Evaluation of Techniques for the Diagnosis of Rotavirus Gastroenteritis in Paediatric Patients

R.K¹,

Oke²,

Anjorin³

et al. 2015

Genes Review

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“…Several rapid tests have been evaluated and showed the ranges of 54-82.9% sensitivity and 73.3-100% specificity. [12][13][14][15][16] The performance of those rapid tests indicated that the tests could only be used for screening but not for confirmation tests. Therefore, a high specific and sensitive test is mostly needed for confirmation of the rotavirus infection.…”

Section: Introductionmentioning

confidence: 99%

Detection of Human Group A and C Rotaviruses in Pediatric Patients with Acute Gastroenteritis by Real Time RT-PCR Assay: A Preliminary Study

Yasmon¹,

Harahap²,

Dwipoerwantoro³

et al. 2011

MJHR

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Rotavirus causes 25-55% of all hospital admissions for diarrhea and approximately 611.000 deaths every year in developing countries. Clinically, it is not possible to recognize the diarrhea caused by rotavirus and other infections. To know a causative agent of rotavirus gastroenteritis, availability of an accurate diagnosis assay is necessary. Therefore, we developed real time RT-PCR assay (rRT-PCR) assay for confirmation of infections of Group A or C rotaviruses simultaneously. A total of 54 stool samples obtained from pediatric patients (< 5 years old) was used in this study. All samples were tested for Group A rotavirus by Serological rapid test. Result of serological rapid test was compared with rRT-PCR assay to obtain the test accuracies of both assays. Result of this study showed that rates of positive testing for Group A rotavirus by serological rapid test and the rRT-PCR assay were 22.22% and 18.50%, respectively. Forty-two serology-negative specimens for Group A rotavirus were also PCR negative (100% specificity). Two serology-positive specimens for Group A rotavirus was rRT-PCR negative (confirmed by electrophoresis gel); therefore, rRT-PCR assay represents the decrease of 3.70% in the number of specimens that are positive for Group A rotavirus. For Group C rotavirus, all tested samples were no rRT-PCR positive and the results need to be confirmed in the future.

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“…Para o diagnóstico laboratorial dos rotavírus são normalmente utilizadas as técnicas de eletroforese em gel de poliacrilamida (PAGE), ELISA, RT-PCR (ESTES; KAPIKIAN, 2007), microscopia eletrônica (ATHANASSIOUS et al, 1994;KAPIKIAN, 2007), aglutinação em látex (BRANDT et al, 1987), imunofluorescência, imunomicroscopia eletrônica (BRIDGE;WOODE, 1975), radioimunoensaio (CUCKOR; BERRY; BLACKLOW, 1978) e contraimunoeletroforese (CANDEIAS; ROSENBURG; RÁCZ, 1978), cada uma com suas vantagens e limitações.…”

Section: Rotavirosesunclassified

“…A aglutinação em látex é uma técnica rápida, simples e de alta especificidade. Porém, apresenta baixa sensibilidade, dependendo do estágio da doença (BRANDT et al, 1987).…”

Section: Rotavirosesunclassified

Ocorrência e caracterização molecular de vírus associados às enteropatias em suínos no Estado de São Paulo

Tonietti¹

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Evaluation of a latex test for rotavirus detection

Cited by 23 publications

References 16 publications

Evaluation of Techniques for the Diagnosis of Rotavirus Gastroenteritis in Paediatric Patients

Evaluation of Techniques for the Diagnosis of Rotavirus Gastroenteritis in Paediatric Patients

Detection of Human Group A and C Rotaviruses in Pediatric Patients with Acute Gastroenteritis by Real Time RT-PCR Assay: A Preliminary Study

Ocorrência e caracterização molecular de vírus associados às enteropatias em suínos no Estado de São Paulo

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