Evaluation of a Microculture Method for Isolation of
            <i>Leishmania</i>
            Parasites from Cutaneous Lesions of Patients in Peru

Boggild, Andrea K.; Miranda‐Verástegui, César; Espinosa, Diego A.; Arévalo, Jorge; Adaui, Vanessa; Tulliano, Gianfranco; Llanos-Cuentas, Alejandro; Low, Donald E.

doi:10.1128/jcm.01286-07

Cited by 38 publications

(54 citation statements)

References 37 publications

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“…The aforementioned study (2004) also expressed that the high sensitivity of the MCM might be explained by the use of capillary tubes, which concentrate the sample material and provide microaerophilic conditions favorable for transformation of the amastigotes to promastigotes. The sensitivity and specificity of the IMC was found to be 98.4 and 100 %, respectively which is comparable with microculture results achieved by previous studies (Allahverdiyev et al 2004;Ihalamulla et al 2005Ihalamulla et al , 2006Boggild et al 2007Boggild et al , 2008Boggild et al , 2010.…”

Section: Discussionsupporting

confidence: 78%

“…While the present study used a new improved, noninvasive, safer (needle free), simpler, faster and more sensitive microculture method compared with studies carried out so far (Ihalamulla et al 2005(Ihalamulla et al , 2006Boggild et al 2007Boggild et al , 2008Boggild et al , 2010 as sampling was taken from the ulcer borders directly and without aspiration by needle.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, in each individual study, the composite reference standard against which each diagnostic test was compared was two of three or four tests with positive results, where tests refer to smears, culture by either method, or PCR. For evaluation of performance characteristics of each test, the lesion was the unit of analysis, as previously described by Boggild et al (2007Boggild et al ( , 2008.…”

Section: Discussionmentioning

confidence: 99%

“…To date, in all performed investigations for MCM evaluation, the samples have been obtained using the aspiration puncture by the syringe. In addition to, these studies had showed varied performance by sensitivity and saving incubation time (Boggild et al 2007(Boggild et al , 2008(Boggild et al , 2010. Therefore, further evaluation and also modification for MCM usefulness in the diagnosis of CL in other endemic countries is needed.…”

Section: Introductionmentioning

confidence: 99%

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An improved microculture method for diagnosis of cutaneous leishmaniasis

et al. 2013

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This study evaluated the performance of three diagnostic methods for cutaneous leishmaniasis (CL). Patients who came to the Health Center Laboratory of Gonbad-e-Qabus in Golestan Province, Iran, were enrolled in the study. Skin scraping smear, improved microculture (IMC) and polymerase chain reaction (PCR) were performed. A total of 303 subjects were recruited, among whom 273 subjects fulfilled the criteria for CL. Sensitivity and specificity were 88.8 % (95 % CI = 84.2-92.2 %) and 100.0 % for smears, 98.4 % (95 % CI = 96.1-99.1 %) and 100.0 % for IMC, both of them 100.0 % for PCR. Although, PCR was relatively more sensitive than the IMC, the high correlation (agreement = 96 %, Kappa = 0.82) between IMC and PCR along with the advantages of simplicity, rapidity, adequate sensitivity and as a needle free method, offers the IMC as a valuable alternative diagnostic method for PCR in diagnosis of CL.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An improved microculture method for diagnosis of cutaneous leishmaniasis

et al. 2013

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“…For the comparative diagnostic evaluation, skin scraping, aspirate specimens, and filter paper lesion impressions were obtained for Giemsa-stained smear, culture, and PCR as described elsewhere. [15][16][17] kDNA PCR of lesion scrapings and aspirates was performed as described using primers specific for Leishmania ( Viannia ) kDNA. [15][16][17][18][19] A single, mid-stream urine specimen was collected from each participant on enrollment before the diagnostic evaluations.…”

mentioning

confidence: 99%

Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis

Veland¹,

Espinosa²,

Valencia³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. We hypothesized that Leishmania kDNA may be present in urine of patients with cutaneous leishmaniasis (CL). Urine samples and standard diagnostic specimens were collected from patients with skin lesions. kDNA polymerase chain reaction (PCR) was performed on samples from patients and 10 healthy volunteers from non-endemic areas. Eighty-six of 108 patients were diagnosed with CL and 18 (21%) had detectable Leishmania Viannia kDNA in the urine. Sensitivity and specificity were 20.9% (95% confidence interval [CI] 12.3-29.5%) and 100%. Six of 8 patients with mucocutaneous involvement had detectable kDNA in urine versus 12 of 78 patients with isolated cutaneous diseaseNo healthy volunteer or patient with an alternate diagnosis had detectable kDNA in urine. Sensitivity of urine PCR is sub-optimal for diagnosis. On the basis of these preliminary data in a small number of patients, detectable kDNA in urine may identify less localized forms of infection and inform treatment decisions.

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Culture of Cutaneous Leishmania from Skin Biopsy Specimens

Paun

2022

Current Protocols

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This protocol describes the culture of Leishmania parasites from skin biopsy samples of patients with cutaneous lesions. The use of antibiotics to prevent bacterial contamination of these cultures increases the ability of researchers to collect isolates for various research purposes, including genetic analysis and in vitro and in vivo experiments. © Published 2022. This article is a U.S. Government work and is in the public domain in the USA. Basic Protocol: Culture of Leishmania from skin biopsy specimens.

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Evaluation of a Microculture Method for Isolation of Leishmania Parasites from Cutaneous Lesions of Patients in Peru

Cited by 38 publications

References 37 publications

An improved microculture method for diagnosis of cutaneous leishmaniasis

An improved microculture method for diagnosis of cutaneous leishmaniasis

Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis

Culture of Cutaneous Leishmania from Skin Biopsy Specimens

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