Evaluation of a one-hour test for the identification of Neisseria species

Lairscey, R; Kelly, M T

doi:10.1128/jcm.22.2.238-240.1985

Cited by 10 publications

(4 citation statements)

References 17 publications

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“…The Rapid Identification Method for Neisseria (RIM-N; Austin Biological Laboratories, Inc., Austin, Tex. ), which has been previously described, consists of micro-carbohydrate (glucose, lactose, sucrose, maltose) utilization reactions with buffered substrates (pH 7.3), with phenol red as the indicator (11,15). In addition to the four carbohydrate substrates including controls, the kit also contained tubes, disposable loops, and a rack.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

1988

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The performance of eight methods in identifying Neisseria species, particularly N. gonorrhoeae, was evaluated. These methods included four rapid carbohydrate utilization tests (Gonobio-Test, Neisseria-Kwik, RIM-N, and Minitek); the Gonochek 11, a test which is based on the utilization of chromogenic substrates; and three monoclonal antibody tests (Syva MicroTrak, GonoGen, and Phadebact Monoclonal GC OMNI Test). In all, 182 isolates comprised in six species of Neisseria as well as Branhamella catarrhalis and Moraxella sp. were tested. Cystine-tryptic digest agar supplemented with sugars was included for reference purposes. In the carbohydrate utilization tests, the sensitivity and specificity of the Neisseria-Kwik and Minitek tests for the identification of N. gonorrhoeae were 100%. This compared with sensitivities and specificities, respectively, of 100 and 99.1% for the Gonobio-Test and 99.1 and 100% for cystine-tryptic digest agar sugars and the RIM-N test. The sensitivity and specificity of the Gonochek II test were 99.0 and 86.7%, respectively. Although most test kits did not claim to identify all Neisseria species, in several cases isolates of N. subflava were misidentified or could be misinterpreted as N. gonorrhoeae or N. meningitidis. With the monoclonal reagents, the Syva MicroTrak system was 100% sensitive and 100% specific. The GonoGen test was both 99.1% sensitive and specific, while the Phadebact Monoclonal GC OMNI Test was 99.1% sensitive but 91.2% specific. With this latter test, cross-reactions were observed with strains of B. catarrhalis, N. cinerea, and N. lactamica.

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Section: Methodsmentioning

confidence: 99%

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

1988

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“…The potentially confusing coccobacillary cells of Moraxella and Acinetobacter species elongate into snakelike cells when division is blocked by sublethal concentrations of penicillins or certain other antibacterial agents. Since elongation does not occur with B. catarrhalis or Neisseria species, this is an easy way to distinguish them from nonsaccharolytic, short paired rods (63 155,185), Gonobio-Test (94), and API QuadFERM+ (109,156). Minitek test reactions were correct in some instances (94), but not all (233).…”

Section: Identification and Biochemical Characteristics Of B Catarrhmentioning

confidence: 99%

Branhamella catarrhalis: an organism gaining respect as a pathogen.

Catlin

1990

Clinical Microbiology Reviews

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“…N. cinerea, another problem organism, also occasionally grows on selective medium (10) and, like N. gonorrhoeae, is prolylaminopeptidase positive, beta-galactosidase negative, and gamma glutamylaminopeptidase negative (1). N. cinerea strains are generally asaccharolytic in carbohydrate degradation test systems, including the 4-h conventional procedure and the 2-h QuadFERM+ system in the present study and the 1-h RIM-N system for Neisseria identification (11). Some strains of N. cinerea, however, have been reported to produce weak positive glucose reactions in some carbohydrate test systems, including the Minitek, RapID-NH, and BACTEC Neisseria differentiation kits (1, 2).…”

Section: Discussionmentioning

confidence: 64%

“…Various modifications of the carbohydrate degradation technique have been developed that allow identification of Neisseria spp. within 1 to 4 h (3,11,13). Most recently, identification methods with specific chromogenic enzyme substrates have become available for rapid identification of Neisseria spp.…”

mentioning

confidence: 99%

API QuadFERM+ with rapid DNase for identification of Neisseria spp. and Branhamella catarrhalis

Janda¹,

Zigler²,

Bradna³

1987

J Clin Microbiol

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The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The two misidentified meningococcal strains were biochemically atypical and were also misidentified by the conventional method. Of 26 N. lactamica strains, 25 (96%) were correctly identified. Of 21 Neisseria spp., 14 (67%) produced carbohydrate reactions in agreement with the conventional procedure, and 7 strains produced detectable acid in the QuadFERM + from maltose and sucrose but not glucose. All 9 N. cinerea and 30 B. catarrhalis strains were asaccharolytic by QuadFERM+. The rapid DNase test was positive for all B. catarrhalis strains and negative for all other organisms. Two beta-lactamase-positive N. gonorrhoeae strains and 25 (93%) of 27 beta-lactamase-positive B. catarrhalis strains were detected by the 2-h acidometric beta-lactamase test on the strip. QuadFERM+ with rapid DNase is a simple and easily interpretable method for identification of these organisms in the clinical laboratory.

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Evaluation of a one-hour test for the identification of Neisseria species

Cited by 10 publications

References 17 publications

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

Branhamella catarrhalis: an organism gaining respect as a pathogen.

API QuadFERM+ with rapid DNase for identification of Neisseria spp. and Branhamella catarrhalis

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