Evaluation of a polymerase chain reaction for the diagnosis of tuberculosis

Manjunath, N; Shankar, Prem; Rajan, Lakshmi; Bhargava, Anudita; Saluja, Sandeep; Shriniwas,

doi:10.1016/0041-3879(91)90020-s

Cited by 115 publications

(53 citation statements)

References 13 publications

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“…In past years, several molecular biology-based techniques have been developed to improve the sensitivity, specificity and speed for M. tuberculosis detection. For example, polymerase chain reaction (PCR) has been used for M. tuberculosis diagnosis in different tests and has been shown to be as sensitive as or better than the gold standard test , Manjunath et al 1991, De Wit et al 1992. The PCR technique involves amplifying different genetic elements used as templates within mycobacteria, such as DNA, rRNA (Jonas et al 1993) or single copy genes encoding 65 or 38 kDa structural proteins (Kaneko et al 1990, Sjobring et al 1990, Brisson-Noël et al 1991.…”

mentioning

confidence: 99%

Mtp-40 and alpha antigen gene fragment amplification for the detection of Mycobacterium tuberculosis in Colombian clinical specimens

Alfonso

Romero

Patarroyo

et al. 2002

Mem. Inst. Oswaldo Cruz

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confidence: 99%

Mtp-40 and alpha antigen gene fragment amplification for the detection of Mycobacterium tuberculosis in Colombian clinical specimens

Alfonso

Romero

Patarroyo

et al. 2002

Mem. Inst. Oswaldo Cruz

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“…The sensitivity and specificity of PCR methods are reported to be far greater than those of the culture method for detection of tuberculosis (21) (12) in a microtitre plate would be more convenient and effecient to perform. In this study we developed a colorimetric PCR assay for the detection A.fumigatus.…”

Section: Discussionmentioning

confidence: 99%

Immuno-polymerase chain reaction for detection ofAspergillus fumigatus

Sarma

Bir

Paliwal

et al. 1997

Indian J Clin Biochem

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“…The primer pair detecting MPB64 coding gene is very popular and specificity for typical and atypical mycobacteria has been reported (Shankar et al 1990; Manjunath et al 1991;Shankar et al 1991;Uematsu et al 1992). Bacteriological specificity of PCR for detection of Mycobacterium tuberculosis complex was tested using commonly isolated non-mycobacterial DNA templates from the respiratory system; these included Staphylococcus aureus, Haemophilus inf luenzae, Moraxella cat arrhalis, Streptococcus pneumoniae, Pseudomonas aeruginosa, a-streptococcus, Candida albicans and human peripheral blood cells.…”

Section: Treatment Of Clinical Samplesmentioning

confidence: 99%

Clinical Evaluation of Polymerase Chain Reaction DNA Amplification Method for the Diagnosis of Pulmonary Tuberculosis in Patients with Negative Acid-fast Bacilli Smear.

Mitarai

Oishi

Fukasawa

et al. 1995

Tohoku J. Exp. Med.

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We evaluated the sensitivity and specificity of polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis among 109 patients who were suspected to have active pulmonary tuberculosis (PTB) and showed negative acid-fast bacilli (AFB) smears in a total of 393 samples of sputum (169), gastric aspirate (134), and urine (90) which fulfilled different criteria for the positivity of PCR. The patients were subsequently divided into one group of active PTB composed of 15 patients with definite PTB and 43 patients with highly suspected PTB, and another group of 51 non-active PTB patients. The PCR assay using samples of sputum and gastric aspirate proved to be specific for active PTB. The PCR method for diagnosis of active PTB using sputum samples was sensitive (97.8%) but lacked specificity (27.0%) when regarded as PCR positive when at least one positive reaction was obtained among all samples examined. However, the PCR of gastric aspirate demonstrated a sensitivity of 63.4% and a specificity of 76.7%. Our data supports that the PCR method for detecting active PTB in AFB smear negative patients using gastric aspirate shows markedly improved sensitivity over the conventional method (25.9%), although it still lacks specificity. PCR assay for M, tuberculosis using multiple samples of gastric aspirate in conjunction with careful clinical observations for the presence of active infection is essential for the diagnosis of active PTB among patients with negative AFB smear.polymerase chain reaction; Mycobacterium tuberculosis; negative acidfast bacilli smear; diagnosis Tuberculosis (TB) is still a serious disease world wide and is becoming even more important with the increasing number of individuals who have the acquired

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Evaluation of a polymerase chain reaction for the diagnosis of tuberculosis

Cited by 115 publications

References 13 publications

Mtp-40 and alpha antigen gene fragment amplification for the detection of Mycobacterium tuberculosis in Colombian clinical specimens

Mtp-40 and alpha antigen gene fragment amplification for the detection of Mycobacterium tuberculosis in Colombian clinical specimens

Immuno-polymerase chain reaction for detection ofAspergillus fumigatus

Clinical Evaluation of Polymerase Chain Reaction DNA Amplification Method for the Diagnosis of Pulmonary Tuberculosis in Patients with Negative Acid-fast Bacilli Smear.

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