Evaluation of a rapid diagnostic test for detection of dengue infection using a single-tag hybridization chromatographic-printed array strip format

Transmission dynamics of dengue in Costa Rica: the role of hospitalizations

“…As such, training of health professional in diagnosis and management in conjunction with laboratory and epidemiological surveillance, is essential [40]. Early accurate notifications of DENV infections will allow health officials to initialized promptly and targeted responses, and continues to highlight the importance and urgent need for the development of specific and sensitive point-of-care test for DENV infections [24].…”

Current vector research challenges in the greater Mekong subregion for dengue, Malaria, and Other Vector-Borne Diseases: A report from a multisectoral workshop March 2019

“…However, patterns of inapparent infection rates are heterogenous, further confounding efforts to estimate transmission intensity [151][152][153][154][155]. While cost-effective and easyto-use rapid diagnostics exist for DENV, a lack of specific and consistently performing diagnostics contributes to this problem, especially in regions where genetically related viruses cocirculate, leading to misclassification of positive results [156][157][158][159][160][161][162][163][164][165][166][167][168][169]. Diagnostic tools for tickborne diseases relevant to the region are nonexistent, and this has hampered confirmation of infection, identification of pathogen, and conduct of surveillance.…”

New Development of Disaster-Related and Tropical Infectious Diseases Control

“…We developed a novel testing, dengue virus RNA detection system using the single tag hybridization chromatographic printed array strip (STH-PAS) method, which affords the visualization of the signals in a single reaction with great sensitivity and in a dramatically short time. Moreover, it does not require the preparation or staining of a gel and an expensive apparatus ( Figure 3) [34]. The methods allow us to detect several genes in one strip so that we can also identify subtypes of DENVs.…”

“…The keynote that the gene can be detected by such a simple visible result is cutting-edge and unique. Sensitivities against serially diluted-DENV subtypes were detected by STH-PAS Dengue Subtype At Once kit using the primers which could detect all subtypes [34]. Virus RNA was obtained using QIAamp Viral RNA Mini Kit (QIAGEN, GmbH, Germany) according to manufacturer's instructions.…”