Evaluation of a rapid test for the hidden hunger of zinc in plants

Dwivedi, R. S.; Randhawa, N. S.

doi:10.1007/bf00011531

Cited by 316 publications

(113 citation statements)

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“…Deficiency of Zn decreases protein synthesis in general rather than affecting particular proteins (Obtat and Umebayashi, 1988;Zhang and Wu, 1989). The rapid recovery in CA activity after resupply of Zn to the Zn-deficient plants is in accordance with reports that the CA holoenzyme could be reconstituted from the apoenzyme present in Zn-deficient tissues upon the resupply of Zn (Dwivedi and Randhawa, 1974;Galdes and Vallee, 1983).…”

Section: I Sc U Sslo Nsupporting

confidence: 91%

Zinc Requirement for Stomatal Opening in Cauliflower

Sharma¹,

Tripathi²,

Bisht³

1995

Plant Physiol.

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Zn deficiency induced increases in epicuticular wax deposits, lamina thickness, degree of succulence, water saturation deficit, diffusive resistance, and proline accumulation and decreases in carbonic anhydrase activity, water potential, stomatal aperture, and transpiration in the leaves of cauliflower (Brassica oleracea L. var botrytis cv Pusa) plants. Restoration of Zn supply to the deficient plants increased stomatal aperture, transpiration, and carbonic anhydrase activity significantly within 2 h. However, leaf water potential in the Zn-deficient plants did not recover within 24 h after resupply of Zn. The guard cells in epidermal peels from the Zndeficient leaves had less K+ than those from the controls. Stomatal aperture in the epidermal peels from Zn-deficient leaves was 64% less than in the controls when the epidermal strips were floated on 125 mM KCI. Supplementing the ambient medium 25 mM KCI with ZnCI, enhanced stomatal aperture in both control and Zn-deficient peels, and the effect was significant in the latter. The observations indicate involvement of Zn in stomatal opening, possibly as a constituent of carbonic anhydrase needed for maintaining adequate [HCO;] in the guard cells, and also as a factor affecting K+ uptake by the guard cells.Previous studies have shown that Zn deficiency stress decreased ij, stomatal aperture, and E and increased WSD in cabbage (Brassica oleracea capitata) (Sharma et al., 1982(Sharma et al., , 1984 and cauliflower (Brassica oleracea botrytis) (Sharma and Sharma, 1987). Hu and Sparks (1991) reported a significant decrease in stomatal conductance in Zn-deficient Stuart pecan leaves. According to Sharma et al. (1982) and Sharma and Sharma (1987), decrease in stomatal opening and transpirational water loss from Zn-deficient cabbage leaves can be attributed to decreased water potential.

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Section: I Sc U Sslo Nsupporting

confidence: 91%

Zinc Requirement for Stomatal Opening in Cauliflower

Sharma¹,

Tripathi²,

Bisht³

1995

Plant Physiol.

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“…Carbonic anhydrase activity was measured by adopting the method of Dwivedi and Randhava. 46 Leaves, used previously for photosynthesis measurements were used for the enzyme assay. Leaves were cut into small pieces (2-3 mm length) in 10.0 ml 0.2 M cysteine in petri dish at 0-4°C.…”

Section: Methodsmentioning

confidence: 99%

Differential cadmium stress tolerance in five Indian mustard (Brassica junceaL.) cultivars

Gill

Khan

Tuteja

2011

Plant Signaling & Behavior

145

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IntroductionAgricultural soils worldwide are slightly to moderately contaminated with toxic heavy metals that restrict the crop plants to reach their full genetic potential and cause significant loss by reducing the crop productivity. 1 Among heavy metals, Cd is a non-essential and most deleterious heavy metal pollutant commonly released into the arable soil from various industrial, mining and farming practices, 2 and has been ranked No. 7 among the top 20 toxins which affect the human health by entering in the food chain. 3,4 Although Cd is a highly phytotoxic metal, it is easily taken up by plant roots growing on Cd-contaminated soils and transported to above ground plant parts. 5-7 The regulatory limit of Cd in agricultural soils is 100 mg Cd kg -1 soil, 8 but this threshold is continuously increasing because of anthropogenic and industrial activities. Plants growing on Cd contaminated soil result in Cd accumulation in all plant parts, which inhibits plant growth, affect nutrient uptake, alters the chloroplast ultrastructure, inactivates enzymes of CO 2 fixation, inhibits photosynthesis and induces lipid peroxidation and antioxidant machinery. 4,[9][10][11] However, Cd is a non redox-active metal, but it induces the generation of reactive oxygen species (ROS) including superoxide radical (O 2•-), hydrogen peroxide (H 2 O 2 ) and hydroxyl radicalThe presence of cadmium (cd) in the agricultural soils affects horticultural cultivars and constrains the crop productivity. a pot experiment was performed using five cultivars of mustard (Brassica juncea L.) to evaluate the difference in their response to cd toxicity under greenhouse conditions. The pots containing reconstituted soil were supplied with different concentration of cdcl 2 (0, 25, 50, 100 or 150 mg cd kg -1 soil). Increasing concentration of cd in the soil resulted in decreased growth, photosynthesis and yield. Maximum significant reduction in growth, photosynthesis and yield were observed with 150 mg cd kg -1 soil in all the cultivars. Our results indicate that the cultivar alankar is found to be more tolerant to cd stress, recording higher plant dry mass, net photosynthesis rate, associated with high antioxidant activity and low cd content in the plant leaves and thus less oxidative damage. cultivar rh30 experienced maximum damage in terms of reduction in growth, photosynthesis, yield characteristics and oxidative damage and emerged as sensitive cultivar. The data of tolerance index of alankar were found to be higher among all tested mustard cultivars which indicate its higher tolerance to cd. Better coordination of antioxidants protected alankar from cd toxicity, whereas lesser antioxidant activity in rh30 resulted in maximum damage. cultivars of mustard were ranked with respect to their tolerance to cd: alankar > Varuna > Pusa Bold > Sakha > rh30, respectively.Differential cadmium stress tolerance in five indian mustard (Brassica juncea L.) cultivars

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“…The activities of nitrate reductase (NR), carbonic anhydrase (CA), catalase (CAT), peroxidase (POX) and superoxide dismutase (SOD) were determined by the method followed by Jaworski (1971), Dwivedi and Randhawa (1974), Chance and Maehly (1956) and Beauchamp and Fridovich (1971), respectively.…”

Section: Determination Of Biochemical Parametersmentioning

confidence: 99%

Brassinosteroid-mediated evaluation of antioxidant system and nitrogen metabolism in two contrasting cultivars of Vigna radiata under different levels of nickel

Yusuf

Fariduddin

Ahmad

et al. 2014

Physiol Mol Biol Plants

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The role of 28-homobrassinolide (HBL) in countering nickel-induced oxidative damage through overexpression of antioxidant enzymes and proline in Vigna radiata has been investigated. Two varieties of V. radiata, one sensitive to Ni (PDM-139) and the other tolerant to Ni (T-44), were sown in the soil fed with different levels (0, 50, 100 or 150 mg kg −1 ) of Ni, and at 29-day stage, foliage of plants was applied with deionized water (control), 10 −8 or 10 −6 M of HBL. The plants were sampled at 45-day stage of growth to assess various physiological as well as biochemical characteristics. The remaining plants were allowed to grow up to maturity to study the yield characteristics. The growth traits, leghemoglobin, nitrogen and carbohydrate content in the nodules, leaf chlorophyll content, photosynthesis efficiency, leaf water potential, activities of nitrate reductase, carbonic anhydrase and nitrogenase decreased proportionately with the increasing concentrations of nickel, whereas electrolyte leakage, various antioxidant enzymes viz. catalase, peroxidase and superoxide dismutase and accumulation of proline increased at 45-day stage. However, the exogenously applied HBL to the nickel-stressed or non-stressed plants improved growth, nodulation and photosynthesis and further enhanced the various antioxidant enzymes viz. catalase, peroxidase and superoxide dismutase and accumulation of proline. The deleterious impact of Ni on the plants was concentration dependent where HBL applied to the foliage induced overexpression of antioxidant enzyme and accumulation of proline (osmolyte) which could have conferred tolerance to Ni up to 100 mg kg −1 , resulting in improved growth, nodulation, photosynthesis and yield attributes.

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Evaluation of a rapid test for the hidden hunger of zinc in plants

Cited by 316 publications

References 4 publications

Zinc Requirement for Stomatal Opening in Cauliflower

Zinc Requirement for Stomatal Opening in Cauliflower

Differential cadmium stress tolerance in five Indian mustard (Brassica junceaL.) cultivars

Brassinosteroid-mediated evaluation of antioxidant system and nitrogen metabolism in two contrasting cultivars of Vigna radiata under different levels of nickel

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