Evaluation of a transfer medium containing high concentration of hyaluronan in human in vitro fertilization

Razi

et al. 2014

Arch Gynecol Obstet

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Routine use of EmbryoGlue® as embryo transfer medium does not improve the ART outcomes

Razi

et al. 2014

Arch Gynecol Obstet

“…However, the effectiveness of HETM is controversial. Two successive studies published in 2006 and 2007 were contradictory: one showed HETM to be effective (increasing implantation and pregnancy rates), while the other reported no such benefits [6,7]. However in both of these studies, patients did not have a history of multiple implantation failures.…”

Section: Introductionmentioning

confidence: 99%

Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Nakagawa¹,

Takahashi²,

Nishi³

et al. 2012

J Assist Reprod Genet

Purpose To ascertain whether the use of hyaluronanenriched transfer medium (HETM) improves pregnancy and implantation rates among embryo transfer patients with a history of multiple implantation failures. Methods Patients (n0314) under the age of 40 and with a history of multiple unsuccessful embryo transfers were enrolled. There were three groups of patients: those undergoing fresh embryo transfer (fresh ET [n 0111]), those undergoing vitrified-warmed ET in the natural cycle (WET-N [n0101]) and those undergoing WET in a hormone replacement cycle ). On the day of ET, patients were randomized to HETM (0.5 mg/ml hyaluronan) or control medium containing no hyaluronan. Only patients with good quality embryos on day 3 were included. Results For all three patients groups (fresh ET, WET-N and WET-H) pregnancy rates (37.5 %, 31.4 % and 41.2 %, respectively) were significantly higher when using HETM compared with control medium (10.9 %, 10.0 % and 15.7 %, respectively; p<0.05), and implantation rates when using HETM were also significantly higher compared with control medium (p<0.05). Miscarriage rates were similar in both groups. Conclusion HETM significantly increased pregnancy and implantation rates among embryo transfer patients with a history of multiple unsuccessful implantations-regardless of method used to prepare the endometrium.

“…On the other hand, some reports contradict the published beneficial effects of HA supplementation in transfer media for embryo transfer (Loutradi et al 2007, Hazlett et al 2008, Check et al 2010. In women who failed to conceive despite at least 3 previous embryo transfers, a 25% clinical pregnancy and 14.2% delivered pregnancy were achieved using EmbryoGlue (highmolecular-weight HA produced by Vitrolife), when compared to women not using EmbryoGlue (39.2% and 39.2% respectively) (Dietterich et al 2007).…”

Section: Embryo Implantation-contrasting Datamentioning

confidence: 99%

Regulation and roles of the hyaluronan system in mammalian reproduction

et al. 2017

Hyaluronan (HA) is a non-sulphated glycosaminoglycan polymer naturally occurring in many tissues and fluids of mammals, including the reproductive system. Its biosynthesis by HA synthase (HAS1-3) and catabolism by hyaluronidases (HYALs) are affected by ovarian steroid hormones. Depending upon its molecular size, HA functions both as a structural component of tissues in the form of high-molecular-weight HA or as a signalling molecule in the form of small HA molecules or HA fragments with effects mediated through interaction with its specific cell-membrane receptors. HA is produced by oocytes and embryos and in various segments of the reproductive system. This review provides information about the expression and function of members of the HA system, including HAS, HYALs and HA receptors. We examine their role in various processes from folliculogenesis through oocyte maturation, fertilisation and early embryo development, to pregnancy and cervical dilation, as well as its application in assisted reproduction technologies. Particular emphasis has been placed upon the role of the HA system in pre-implantation embryo development and embryo implantation, for which we propose a hypothetical sequential model. Reproduction (2017) 153 R43-R58