Evaluation of a Triplex PCR Assay To Discriminate<i>Staphylococcus aureus</i>from Coagulase-Negative Staphylococci and Determine Methicillin Resistance from Blood Cultures

Maes, Nicole; Magdalena, Juana; Rottiers, S.; Gheldre, Yves De; Struelens, Marc

doi:10.1128/jcm.40.4.1514-1517.2002

Cited by 194 publications

(154 citation statements)

References 25 publications

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“…Bacterial DNA was extracted as described by Ü nal et al (16), and then all isolates were tested for the presence of the mec(A) gene as previously described (17). The presence of the antibiotic resistance genes aph(3=)-…”

Section: Methodsmentioning

confidence: 99%

Comparative Epidemiology of Staphylococcus epidermidis Isolates from Patients with Catheter-Related Bacteremia and from Healthy Volunteers

et al. 2013

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Staphylococcus epidermidis is a major cause of catheter-related bloodstream infections (CRBSIs). Recent studies suggested the existence of well-adapted, highly resistant, hospital-associated S. epidermidis clones. The molecular epidemiology of S. epidermidis in Belgian hospitals and the Belgian community has not been explored yet. We compared a set of 33 S. epidermidis isolates causing CRBSI in hospitalized patients with a set of 33 commensal S. epidermidis isolates. The factors analyzed included resistance to antibiotics and genetic diversity as determined by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and SCCmec typing. Additionally, the presence of virulence-associated mobile genetic elements, the ica operon and the arginine catabolic mobile element (ACME), was assessed and compared against clinical data. CRBSI S. epidermidis isolates were significantly resistant to more antibiotics than commensal S. epidermidis isolates. The two populations studied were very diverse and genetically distinct as only 23% of the 37 PFGE types observed were harbored by both CRBSI and commensal isolates. ACME was found in 76% of S. epidermidis strains, regardless of their origin, while the ica operon was significantly more prevalent in CRBSI isolates than in commensal isolates (P < 0.05). Nine patients presented a clinically severe CRBSI, eight cases of which were due to an ica-positive multiresistant isolate belonging to sequence type 2 (ST2) or ST54. S. epidermidis isolates causing CRBSI were more resistant and more often ica positive than commensal S. epidermidis isolates, which were genetically heterogeneous and susceptible to the majority of antibiotics tested. Clinically severe CRBSIs were due to isolates belonging to two closely related MLST types, ST2 and ST54.

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Section: Methodsmentioning

confidence: 99%

Comparative Epidemiology of Staphylococcus epidermidis Isolates from Patients with Catheter-Related Bacteremia and from Healthy Volunteers

et al. 2013

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“…DNA was extracted from all isolates as previously described (Vanderhaeghen et al, 2010b). MRSA identification was performed using a triplex polymerase chain reaction (PCR), previously published by Maes et al (2002). This PCR allows detection of the staphylococcal-specific 16S rRNA gene, the nuc gene specific for S. aureus, and the presence of the mecA gene responsible for methicillin resistance.…”

Section: Methodsmentioning

confidence: 99%

Characterization of methicillin-resistantStaphylococcus aureusfrom healthy carrier chickens

et al. 2013

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“…Inducible or constitutive lincomycin resistance was determined by the double-disk diffusion test (D-test). The presence of the mecA gene was determined by PCR in all cefoxitinresistant isolates, as previously described [9]. S. aureus ATCC 43300 was used as the positive control.…”

Section: Antibiotic Susceptibility Testing Methodsmentioning

confidence: 99%

“…Suspicious colonies were identified as S. aureus by colony morphology and catalase and DNase tests. Presumptive S. aureus were confirmed by PCR for 16S rRNA and nuc genes, as previously described [9]. S. aureus ATCC29213 was used as the positive control.…”

Section: Isolation and Identification Of Nasal S Aureus Isolatesmentioning

confidence: 99%

Nasal carriage of sequence type 22 MRSA and livestock-associated ST398 clones in Tangier, Morocco

Mourabit¹,

Arakrak²,

Bakkali³

et al. 2017

J Infect Dev Ctries

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Introduction: This study aimed to provide data of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage as well as to determine the genetic lineages of this circulating MRSA in the Tangier community. Methodology: Between 2012 and 2013 two subpopulations consisting of randomly chosen healthy volunteers and outpatients in 11 healthcare facilities were screened. The antibiotic resistance phenotype was determined by disk diffusion. Toxin Panton-Valentin Leukocidin (PVL), toxic shock syndrome toxin-1 gene (tst), and mecA were detected by polymerase chain reaction (PCR). Nasal swabs were obtained from persons with no identified risk factors for MRSA acquisition. MRSA molecular typing was performed by pulsed-field gel electrophoresis (PFGE), staphylococcal chromosomal cassette mec, and Staphylococcus protein A (spa) typing. Results: A total of400 subjects (33.3%) were nasally colonized with S. aureus, and 17 (1.4%) were nasal carriers of MRSA. The analysis did not identify age, gender, and the two subpopulations as predictors for MRSA colonization. MRSA were more likely to harbor the tst gene (p < 0.05). This work highlighted a low prevalence of nasal MRSA carriage, with 52.94% belonging to sequence type (ST) ST22. The remaining isolates were distributed as singletons (ST8, ST1, and ST398), whereas approximately one-third of MRSA was not identified, including three novel spa-types (t13247, t13248, and t13249). Conclusions: Although we highlighted the current clones present in the Tangier community, they are limited in space and time. Therefore, further studies would be required to obtain a comprehensive picture of the dissemination of MRSA in the community, hospital, and livestock.

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Evaluation of a Triplex PCR Assay To DiscriminateStaphylococcus aureusfrom Coagulase-Negative Staphylococci and Determine Methicillin Resistance from Blood Cultures

Cited by 194 publications

References 25 publications

Comparative Epidemiology of Staphylococcus epidermidis Isolates from Patients with Catheter-Related Bacteremia and from Healthy Volunteers

Comparative Epidemiology of Staphylococcus epidermidis Isolates from Patients with Catheter-Related Bacteremia and from Healthy Volunteers

Characterization of methicillin-resistantStaphylococcus aureusfrom healthy carrier chickens

Nasal carriage of sequence type 22 MRSA and livestock-associated ST398 clones in Tangier, Morocco

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