Evaluation of Adjuvants for Increased Efficacy of HearNPV Against Helicoverpa armigera (Hubner) Using Suntest Machine

Mehrvar, Ali; Rabindra, R. J.; Veenakumari, K.; Narabenchi, G. B.

doi:10.3923/jbs.2008.534.541

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Viral insecticides cannot be developed commercially until their formulations are physically, chemically, and environmentally stable in storage and distribution. Use of adjuvants has been found to increase the persistence of the virus in the environment (Mehrvar et al 2008). The incorporation of adjuvants with microbial insecticides to preserve the virus activity is commonly followed (Rabindra and Jayaraj 1995).…”

Section: Discussionmentioning

confidence: 99%

Development of novel betabaculovirus (HycuGV-Hc1) as a biopesticide (HycuGV-TR61) and its efficacy on the fall webworm, Hyphantria cunea Drury (Lepidoptera: Erebidae) larvae

Bayramoglu

Gencer²,

Demir

2023

Egypt J Biol Pest Control

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Background The aim of this study was to develop an oil formulation from a local betabaculovirus. Hyphantria cunea Drury (Lepidoptera: Erebidae) granulovirus (HycuGV-Hc1) was isolated from the infected larvae to test its efficacy on the pest. The oil formulation was prepared by mixing the viral suspension with sunflower oil and some adjuvants and named HycuGV-TR61. Crude virus and the formulations were carried out on third instar H. cunea larvae using 1 × 104–8 OBs /ml concentrations and were exposed to temperatures (28, 35, and 42 °C) and UV-B light at different periods (0, 1, 3, 5 h). Results The mortality rate, which was 50% at the lowest concentration (1 × 104 OB/ml), reached 99.86% at the highest concentration (1 × 108 OB/ml). LC50 values of fresh and old forms were calculated as 0.64 × 104 and 0.87 × 104 OBs/ml, respectively. Application of shelf life showed that there was non-significant change in the pathogenic activity of the formulation with time. In the experiments, it was observed that the activity decreased as the temperature and time of exposure increased. Significantly difference in larval mortality was observed when fresh and old formulations were exposed to 0, 1, 3 and 5 h to UV-B, (old: 96.7, 86, 80 and 60%; fresh: 97.1, 90, 85 and 62%, respectively). Conclusions The results revealed superior aspects of HycuGV-TR61, which was developed as a local viral biopesticide, its resistance to abiotic factors and its potential to be used in pest control.

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Section: Discussionmentioning

confidence: 99%

Development of novel betabaculovirus (HycuGV-Hc1) as a biopesticide (HycuGV-TR61) and its efficacy on the fall webworm, Hyphantria cunea Drury (Lepidoptera: Erebidae) larvae

Bayramoglu

Gencer²,

Demir

2023

Egypt J Biol Pest Control

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“…Menururt Bedjo (2005) kandungan flavonoid dan sukrosa pada molase dapat Author(s): Nurliyani Anggeraini, Irma Wardati, Nisa Budi Arifiana _______________________ melindungi Sl-NPV dari paparan sinar. Hasil penelitian (Mehrvar et al, 2008) juga menunjukan bahwa molase 5% memiliki nilai ER sebesar 1,8 yang hampir sama dengan penelitian ini yaitu 1,64 dengan rata-rata mortalitas 90,00%. Kaolin memiliki nilai ER sebesar 1,45 dengan mortalitas 80,00% Kaolin berpengaruh sebagai protektan Sl-NPV disebabkan kaolin memiliki sifat daya hantar panas yang rendah, sehingga dapat melindungi Sl-NPV dari pengaruh sinar UV yang dapat menurunkan keefektifan Sl-NPV (Bulan et al, 2014).…”

Section: Pendahuluanunclassified

UJI VIRULENSI Spodoptera litura F. NUCLEAR POLYHEDROSIS VIRUS (S1-NPV) DENGAN PENAMBAHAN ULTRA VIOLET PROTEKTAN TERHADAP ULAT DAUN TEMBAKAU (Spodoptera litura F.)

2022

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Sl-NPV is a specific type of NPV used to control S.litura. Exposure to ultraviolet light causes a decrease in the effectiveness of Sl-NPV, so a protective material is needed that can maintain the virulence of Sl-NPV from UV exposure. The purpose of this study was to determine the virulence of Sl-NPV with the addition of a protective agent and to determine the most efficient protective agent for the virulence of Sl-NPV against S.litura. This study used a Randomized Block Design (RAK) consisting of one treatment factor, namely the type of protective material, with 6 treatments, namely control, Sl-NPV+kaolin 5%, Sl-NPV+ husk charcoal 5%, Sl- NPV + molasses 5%, Sl-NPV + yam extract 5%, Sl-NPV + cucumber extract 5%. The observation parameters used in this study were mortality of S. litura larvae, LT50, ER, and physical changes. The results showed that the addition of a protective agent (uv protector) had an effect on Sl-NPV in causing mortality of S. litura F. The most efficient protective agent was molasses with an ER value of 1.64 and an LT50 of 54 hours.

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“…Isolat-isolat virus yang memiliki virulensi tinggi mampu mematikan larva inangnya dalam waktu 2 -5 hari, tetapi isolat yang kurang virulen membutuhkan 2 -3 minggu (Granados dan William, 1986 (Bonning dan Hammock, 1996;Dushoff dan Dwyer, 2001), memiliki inang yang spesifik, sehingga terlalu mahal untuk dikembangkan dalam skala industri (McCutchen et. al., 1991) dan cepat menjadi tidak aktif di lapangan akibat sinar ultra violet (UV) dari matahari (Ignoffo et al, 1991;Monobrullah, 2003;McIntosh et al, 2004;Mondragon et al, 2007;Mehrvar et al, 2008).…”

Section: Gejala Infeksi Npvunclassified

“…Beberapa bahan telah diuji untuk mempertahankan persistensi NPV terhadap paparan sinar ultraviolet (UV), antara lain: pencerah fluorescen (fluorescent brightener) pada Spodoptera frugiperda NPV (SfNPV) (Hamm et al, 1994;Martinez et al, 2003;Mondragon et al, 2007), Lymantria dispar NPV (LdNPV) (Dougherty et al, 2006), S. exigua NPV (SeNPV) (Kao et al, 1991;Murillo et al, 2003;Lasa et al, 2007), Titanium dioksida (TiO2) pada Helicoverpa zea NPV (HzNPV) (Farrar et al, 2004), oksida besi pada Homona magnanima GV (HomaGV) (Asano, 2005), Congo red dan Tinopal LPW pada L. dispar NPV (LdMNPV) (Shapiro dan Shepard, 2008), adjuvan pada H. armigera NPV (HaNPV) (Mehrvar et al 2008), ekstrak teh hijau pada S. exigua NPV (SeNPV) , ekstrak teh, kopi dan kakao pada S. exigua (SeNPV) (El Salamouny et al, 2009), dan filtrat bengkuang pada S. exigua (SeNPV) (Samsudin et al, 2011 (Dodin et al, 2001;Samsudin, 2001;Arifin, 2006). Hasil beberapa penelitian menunjukkan bahwa NPV bekerja secara sinergis dengan ekstrak biji mimba (Azadirachta indica) (Dodin et al, 2001;Kumar et al, 2007), nematoda Steinernema carpocapsae (Gothama et al, 1996), bakteri Pseudomonas fluorescen (Jeyarani et al, 2005) dan jamur Metarhizium anisopliae (Ramle et al, 2005).…”

Section: Gejala Infeksi Npvunclassified

PROSPEK PENGEMBANGAN BIOINSEKTISIDA NUCLEOPOLYHEDROVIRUS (NPV) UNTUK PENGENDALIAN / Prospect of Development of Nucleopolyhedrovirus (NPV) Bioinsecticide Against

Samsudin¹

2017

PSP

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ABSTRAKPenggunaan insektisida kimia untuk mengendalikan hama tanaman perkebunan terbukti kurang efektif, tidak praktis, mahal, dan mencemari lingkungan. Virus patogen serangga (VPS) terutama nucleopolyhedrovirus (NPV) memiliki potensi untuk dikembangkan menjadi bioinsektisida yang efektif, efesien dan ramah lingkungan. NPV memiliki kemampuan bertahan di alam karena partikel virusnya terbungkus oleh polihedra dan mampu menyebar secara alami melalui proses penularan vertikal dan horisontal. Larva yang mati terinfeksi NPV sering ditemukan menggantung dengan kedua tungkai semu bagian abdomen menempel pada daun atau ranting tanaman. Penelitian untuk meningkatkan virulensi, spektrum inang dan persistensinya telah dilakukan untuk mengatasi beberapa kelemahan NPV apabila dikembangkan menjadi bioinsektisida. Beberapa NPV yang menginfeksi hama tanaman perkebunan dan berpotensi untuk dikembangkan di Indonesia, antara lain: Spodoptera litura NPV (SlNPV), S. exigua NPV (SeNPV), Helicoverpa armigera NPV (HaNPV), Sethotosea asigna NPV (SaNPV), Hyposidra talaca NPV (HtNPV) dan Maenas maculifascia NPV (MmNPV). Berdasarkan beberapa keunggulan NPV dibandingkan dengan insektisida kimia, maka pengembangan bioinsektisida NPV untuk pengendalian hama tanaman perkebunan di Indonesia memiliki prospek yang sangat baik.

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Evaluation of Adjuvants for Increased Efficacy of HearNPV Against Helicoverpa armigera (Hubner) Using Suntest Machine

Cited by 6 publications

References 25 publications

Development of novel betabaculovirus (HycuGV-Hc1) as a biopesticide (HycuGV-TR61) and its efficacy on the fall webworm, Hyphantria cunea Drury (Lepidoptera: Erebidae) larvae

Development of novel betabaculovirus (HycuGV-Hc1) as a biopesticide (HycuGV-TR61) and its efficacy on the fall webworm, Hyphantria cunea Drury (Lepidoptera: Erebidae) larvae

UJI VIRULENSI Spodoptera litura F. NUCLEAR POLYHEDROSIS VIRUS (S1-NPV) DENGAN PENAMBAHAN ULTRA VIOLET PROTEKTAN TERHADAP ULAT DAUN TEMBAKAU (Spodoptera litura F.)

PROSPEK PENGEMBANGAN BIOINSEKTISIDA NUCLEOPOLYHEDROVIRUS (NPV) UNTUK PENGENDALIAN / Prospect of Development of Nucleopolyhedrovirus (NPV) Bioinsecticide Against

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