Evaluation of an Automated, High Throughput Flowcytometry Based Technique for Diagnosis of Malaria

Negi, Arti; Mirdha, Bijay Ranjan; Arulselvi, S; Singh, Avinash; Patel, Sangram Singh; Agarwal, Anjali; Samantaray, Jyotish Chandra

doi:10.20546/ijcmas.2018.706.112

Cited by 2 publications

(3 citation statements)

References 9 publications

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“…In malaria positive cases out of 20 malaria positive cases 16 were male and 4 were female. Analysis of present study was consistent with Arti Negi et al 2018 [9] in which male: female ratio was 1.8:1.…”

Section: Demographic Profile Of Patientssupporting

confidence: 91%

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Utility of Automated Hematology Analyzers in Diagnosis of Infective Etiologies: An Adjunct to Conventional Diagnostic Tools

Patel¹,

Ranapurwala²,

Mansuri³

et al. 2021

Ann of Pathol and Lab Med

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Background: Febrile illnesses such as malaria and dengue are challenging to differentiate clinically. For more than a century, the diagnostic approach is by clinical features and microscopy or manual immune-chromatographic tests. Automated cellular (WBC) indices from hematology analyzers may afford a preliminary rapid distinction. Several abnormal scattergrams during routine CBC analysis appear for the malaria and dengue. Material and Methods: The present study is an observational, prospective study undertaken to evaluate the diagnostic utility of Sysmex XN-L series hematology analyzer as a screening tool to trigger specific testing for malaria and dengue. It includes patients suffering from either of the two febrile illnesses (malaria or dengue) at Hematology and microbiology section of Central Diagnostic Laboratory of a tertiary care centre in Central Gujarat. The sensitivity, specificity, PPV and NPV was performed for each abnormal pattern noted on scattergram for malaria and/or dengue. Interobserver variation is also taken in consideration. Results: Malaria cases showed a significant statistical association with the presence of an extended neutrophil zone and dengue positive cases with NS1 antigen, IgG and IgM antibodies testing showed significant association with the hourglass pattern. Higher degree of agreement was noted for the hourglass pattern, doubling of neutrophil zone pattern among the observers Conclusion: Significant statistical correlation was observed for extended neutrophil zone and malaria cases, hourglass pattern and dengue cases with a good agreement among the observers.

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Section: Demographic Profile Of Patientssupporting

confidence: 91%

“…In the study done by Arti Negi et al (2018) [9] and Huh et al (2008) [10] only malaria cases were included. On the contrary, in the present study we have included both malaria and dengue cases (Table -4).…”

Section: Scattergram Patternsmentioning

confidence: 99%

Utility of Automated Hematology Analyzers in Diagnosis of Infective Etiologies: An Adjunct to Conventional Diagnostic Tools

Patel¹,

Ranapurwala²,

Mansuri³

et al. 2021

Ann of Pathol and Lab Med

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“…For malaria diagnosis, this technique, like Laser Desorption Mass Spectrometry depends on the detection and quantification of heme using a special machine called flow cytometer and also works on the principle of flow cytometry, hence, the name of the technique. This is a haematology analyser that is used for the presumptive diagnosis of malaria and other haematological parameters of the sample [74]. Different flow cytometric methods are used to determine the presence of the infection and also quantify the level of parasitaemia [75].…”

Section: Flow Cytometrymentioning

confidence: 99%

Advanced Techniques and Unusual Samples for Malaria Diagnosis

Muhammad,

Pukuma Sale,

Mahmoud Mohammed

2024

Infectious Diseases

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Successful malaria control, treatment, and prevention depends on successful diagnosis using appropriate equipment with high sensitivity and specificity. In most tropical countries where the disease is endemic, malaria diagnosis is still based on the conventional techniques (Microscopy and RDT) which have so many shortcomings, hence the need to switch to the most advanced diagnostic technique for better results. In this review, several serological and molecular malaria diagnostic techniques like Polymerase Chain Reaction (PCR), Flow cytometry, Loop-mediated Isothermal Amplification (LAMP), Indirect Immunofluorescence, Enzyme-Linked Immunosorbent Assay (ELISA), Radioimmunoassay (RIA), Quantitative Buffy Coat (QBC) and Laser Desorption Mass Spectrometry (LDMS) were systematically discussed in simple and direct language for easier understanding of the principle involved in each case scenario. In addition, some unusual samples for malaria diagnosis like Urine and saliva were also discussed.

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Evaluation of an Automated, High Throughput Flowcytometry Based Technique for Diagnosis of Malaria

Cited by 2 publications

References 9 publications

Utility of Automated Hematology Analyzers in Diagnosis of Infective Etiologies: An Adjunct to Conventional Diagnostic Tools

Utility of Automated Hematology Analyzers in Diagnosis of Infective Etiologies: An Adjunct to Conventional Diagnostic Tools

Advanced Techniques and Unusual Samples for Malaria Diagnosis

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