Evaluation of an immunochromatography method for rapid detection of noroviruses in clinical specimens in Thailand

Abstract

Norovirus (NoV) is a causative agent of gastroenteritis in children and adults worldwide. Although reverse transcription-polymerase chain reaction (RT-PCR) has been accepted as the standard method for diagnosis of NoV infection, it requires well-trained personnel and sophisticated equipments. Performance of a commercial immunochromatography (IC) test for rapid detection of NoV was evaluated with fecal specimens collected from children admitted to a hospital with acute gastroenteritis during 2005-2007 in Chiang Mai, Thailand. A total of 463 fecal specimens were tested for the presence of NoV by a commercial immunochromatography kit (IP-NoV) and by RT-PCR. Sensitivity, specificity, and agreement of immunochromatography as compared to RT-PCR were 74.2%, 99.5%, and 96.1%, respectively. Based on the NoV genotypes determined by phylogenetic analysis, immunochromatography detected NoV GII/3, GII/4, GII/6, GII/13, GII/15, and GII/16 genotypes. The findings indicate that the immunochromatography kit could be used for a direct detection of NoV GII in clinical specimens and covering a wide range of NoV genotypes circulating in Thailand.

Human norovirus genogroup II recombinants in Thailand, 2009–2014

“…This rate was comparable to those found in other Thai studies [16]. Furthermore, GII.4 was the most common NoV genotype identified in this and many previous studies on stool samples done between 2002 and 2011 [19][20][21][22]27].…”

Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application

“…Rapid and accurate detection methods are essential for response to viral diarrhea outbreaks and management of the patients (12,13). Current detection methods include electron microscopy (14), enzyme immunoassay (EIA) (15), conventional or real-time PCR (rtPCR) (16)(17)(18)(19), and Luminex xMAP technology (12,13). These methods are laborious (14) or need post-gel electrophoresis (18) or allow simultaneous detection of three or four viral pathogens in one reaction (16,17,19) or need opening of reaction tubes for hybridization (12,13).…”

Incidence of Rotavirus, Enteric Adenovirus and Norovirus in Children with Acute Gastroenteritis Under Five Years

“…2010 yılında İngiltere'de geçmişe yönelik taranan örneklerde %4.5 oranında norovirüs belirlemişlerdir (34) . Çin, Kore ve Tayvan gibi Asya ülkelerinde 2005-2009 yılları arasında yapılan çalışmalarda %13.1-45 oranında norovirüs pozitifliğini göstermişlerdir (36)(37) .…”