Evaluation of Anti-inflammatory Effect of Ashwagandha: A Preliminary Study in vitro

Abstract

the development of modern medicine. Recently traditional medicine worldwide is being re-evaluated by extensive research on different plant species and their active therapeutic principles. The rich wealth of plant kingdom can represent a novel source of newer compounds with significant antiinflammatory activities. The major merits of herbal medicine seem to be their perceived efficacy, low incidence of serious adverse effects and low cost. Ashwagandha, also known as Indian ginseng and winter cherry, consists of dried roots of Withania somnifera (L.) Dunal. (Family: Solanaceae). It is a perennial plant indigenous to India, grown and cultivated throughout subtropical India. It has been recognized as an important herb in the Ayurveda, the traditional system of Indian medicine for more than 3000 years. Traditionally it has been used for several important medicinal purposes in the Indian subcontinent. Recently there has been renewed interest on ashwagandha for its effectiveness in several disease conditions, adaptogenic, immunomodulator and other health benefits. [4,5] Previous researchers have reported several pharmacological properties of ashwagandha on animals and humans. [6-10] The present study was conducted to evaluate the anti-inflammatory effect of ashwagandha extract against the denaturation of protein in vitro.

Determination of permeability, plasma protein binding, blood partitioning, pharmacokinetics and tissue distribution of Withanolide A in rats: A neuroprotective steroidal lactone

“…It has been widely used in different forms of drug preparation from ancient time to improve the human longevity and vitality (Kulkarni & Dhir, ; Sangwan et al, ). Recent pharmacological studies have revealed that W. somnifera possess innumerable pharmacological actions including anti‐inflammatory (Chandra, Chatterjee, Dey, & Bhattacharya, ), antioxidant (Dhanani, Shah, Gajbhiye, & Kumar, ), antihypertensive (Andallu & Radhika, ), neuroprotective (Ahmad et al, ), immune modulation (Agarwal, Diwanay, Patki, & Patwardhan, ), anticancer (Jayaprakasam, Zhang, Seeram, & Nair, ; Rai, Jogee, Agarkar, & CAd, ) activities and also help in improvement of stress‐related disorders (Archana & Namasivayam, ). The therapeutic potential of W. somnifera is mainly due to the presence of various active moieties having a steroidal lactone ring in their structure called Withanolide (22‐hydroxyergostan‐26‐oic acid‐26,22‐lactone) (Mirjalili, Moyano, Bonfill, Cusido, & Palazón, ).…”

Assessment of in vitro antiarthritic activity of Manilkara hexandra (Roxb.) Dubard leaf extract

“…This antidenaturation effect was further supported by the change in viscosities. It has been reported that the viscosities of protein solutions increase on denaturation (Sangeetha et al, 2011;Chandra et al, 2012).…”

In Vitro Anti-Inflammatory and Anti-Diabetic Activity of Methanolic Extract of Cardanthera Difformis Druce

“…The samples were incubated at 37˚ C for 15 minutes and heated at 70 ˚ C for 5 minutes. After cooling, the absorbance of turbidity was measured at 660 nm in UV-vis spectrophotometer the above solutions percentage inhibition of protein denaturation was calculated using the following formula 23 .…”

“…It is the body response to inactivate or destroy the invading organism, to remove the irritants and set the stage for tissue repair. It is triggered by the release of chemicals mediators from injured tissue and migrating cells 3 . The production of auto antigens in certain arthritic diseases may be due to in vivo denaturation of proteins 4 .…”