Evaluation of Anti-Microbial Activity and Proximate Composition of Alkaloids from Vitex doniana Seed

Abstract

The study investigated the in vitro anti-microbial activity of crude alkaloids from the seeds of Vitex doniana against some selected bacteria and fungal strains. Agar well diffusion method was used to test the antimicrobial activity using ciprofloxacin and ketoconazole as controls. Phytochemical and proximate analyses were carried out by standard methods. The crude extract showed the presence of alkaloids, tannins and resins in abundance. Proximate analysis indicated fat/oil (11±0.73 %), protein (0.26±0.02 %), moisture (0.21±0.01 %), ash (5.0±0.22 %) and carbohydrate (6.42±0.28 %). The antimicrobial study indicated that the crude extract was more effective against Pseudomonas aeruginosa (MIC 3.84 mg/ml) and Salmonella typhi (MIC 3.84 mg/ml) and other Gram-positive bacteria. The crude alkaloids generally showed lower activity in case of Gram-negative (MIC Salmonella typhi 4.20 mg/ml) than in Gram-positive bacteria (MIC Staphylococcus aureus 2.52 mg/ml). Surprisingly, the crude alkaloids from the seeds, in addition to improved activity against all the bacteria strains, showed significant activity against Candida albicans (MIC 1.18 mg/mL. V. doniana seed extract was found to be potent against some clinical strains of both Gram-positive and negative bacteria but not fungi; however, its alkaloids has promising antifungal activity.
 Dhaka Univ. J. Pharm. Sci. 20(1): 81-86, 2021 (June)

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“…The present study showed a good antibacterial potential of the plant fraction extracts on these different microorganisms studied. Indeed, the results show that the MICs obtained on B. subtilis, S. aureus, P. aeroginosa and S. Typhi bacteria are better to those obtained by Udeani et al (2021) who obtained MICs for B. subtilis (28.15 mg/mL), S. aureus (8.90mg/mL), P. aeroginosa (3.84mg/L) and S. Typhi (3.80mg/mL) respectively. It is noted that anti-bacterial activity was found to be more pronounced against Gram + bacteria compared to Grambacteria.…”

Bio-guided Fractionation: Optimization of Chemical Profiling, Antioxidant, Anti-inflammatory and Antibacterial Properties of Vitex doniana Fruits

“…The present study showed a good antibacterial potential of the plant fraction extracts on these different microorganisms studied. Indeed, the results show that the MICs obtained on B. subtilis, S. aureus, P. aeroginosa and S. Typhi bacteria are better to those obtained by Udeani et al (2021) who obtained MICs for B. subtilis (28.15 mg/mL), S. aureus (8.90mg/mL), P. aeroginosa (3.84mg/L) and S. Typhi (3.80mg/mL) respectively. It is noted that anti-bacterial activity was found to be more pronounced against Gram + bacteria compared to Grambacteria.…”

Toxicological profile, phytochemical analysis and anti-inflammatory properties of leaves of Vitex doniana Sweet. (Verbenaceae)

“…V. doniana Sweet., is also thought to be used in treatments for jaundice, anemia, dysentery, mental illness, rheumatism, gastrointestinal disorders and urinary conditions, etc [ 11 ]. In addition, the bark of the plant's stem has antibacterial properties [ 12 ]. In Burkina Faso, V. doniana Sweet., is a food plant used to treat inflammatory diseases and certains cancers [ 8 ].…”

“…In Burkina Faso, V. doniana Sweet., is a food plant used to treat inflammatory diseases and certains cancers [ 8 ]. Some studies show that phytochemical analysis of the different parts of the plant extract revealed the presence of certain secondary metabolites such as: saponin, tannins, phenols, cardiac glycosides, flavonoids, sterols and triterpenes as well as a high concentration of sodium, potassium, calcium, iron, phosphorus and sulfur [ 10 , 11 , 12 , 13 ]. In order to find a new remedy for inflammation, this work aims to determine the scientific basis for the traditional use of V. doniana .…”