Evaluation of antibacterial activity and acute toxicity of pomegranate (punica granatum l.) seed ethanolic extracts in swiss webster mice

Abstract

Objective: To evaluate the safety of a pomegranate seeds ethanolic extract in Swiss webster mice. Material and Methods: Pomegranate seed's ethanolic extracts were evaluated for minimum inhibitory concentration against streptococcus sanguis ATCC 10556, using microdilution method through 96-wells microplate. In acute toxicity test, 12 females and 12 males Swiss Webster mice weighing 20-30 grams were divided into four groups, each consisting of 3 females and 3 males. Three groups were orally given of sample with concentrations 0.2% equivalent to 40 mg/kg, 0.4% equivalent to 80 mg/kg, and 0.8% equivalent to 160 mg/kg, while the control group was given distilled water. Observations were made at 30, 60 and 90 minutes, for 24 hours and 7 days. Results: The MIC against streptococcus sanguis was on 2000 ppm concentration and until 24 hours observation no lethal effect occurred on the mice, but at the 7 days observation in the group of 0.8% per kilogram body weight, the lethal effect occurred at one mice. Conclusion:The ethanolic extract of pomegranate seeds has antibacterial effect and no acute systemic toxicity, which can be considered safe on the use as oral topical solution since the toxic dose is greater than 160 mg/kg of body weight.Keywords: Pomegranate, Ethanolic extract, Acute systemic toxicity Cite this Article: Setiadhi, R., Sufiawati, I., Zakiawati, D., Nur'aeny, N., Hidayat, W., Firman, D.R. 2017. Evaluation of antibacterial activity and acute toxicity of pomegranate (punica granatum l.) seed ethanolic extracts in swiss webster mice.

Bactericidal, protistocidal, nematodicidal properties and chemical composition of ethanol extract of Punica granatum peel

“…During testing ethanol extract from fruits and seeds of P. granatum on white mice, Satheesh Kumar Bhandary et al (2013) observed no death or changes in behaviour, impairments in parameters of blood and histological deviations in the liver and kidneys (the authors used extracts with internal administration by 2 g/kg of body weight daily over 28 days). Ethanol extract from seeds of P. granatum had no sharp systemic toxicity at consumption by mice in the dose of over 160 mg/kg of body weight during 7 days observation (Setiadhi et al, 2017).…”

Toxicological Screening of Ellagic Acid in Pomegranate Fruit and Hydroxyapatite Combination as Bone Graft Material on BHK-21 Fibroblast Cell

“…29 Other studies also revealed non-toxic property of EA towards RAW264.7 cells which is a progenitor for bone and fibroblast formation. 30,31 In this study, cell viability was less than 100% showing the presence of cell death that yet to be classified as toxic. This occurs as the result of cytotoxic effect from EA material as it initiates changes in the permeability of fibroblast cell membrane.…”

Antimicrobial, antioxidant activities and toxicity on Cavia porcellus of Dialium angolense Welw. Ex Oliv, a traditional medicinal plant from Bagira in Eastern of DR Congo

“…Many plants used in traditional medicine with both antioxidant and antimicrobial potential like Punica granatum L., Lythraceae [76], Feijoa sellowiana (O.Berg) O.Berg, Myrtaceae [77] , Xylopia aethiopica (Dunal) A.Rich., Annonaceae [78], Zingiber officinale Roscoe, Zingiberaceae [79] or Fagaropsis hildebrandtii (Engl.) Milne-Redh., Rutaceae [80], for which LD50 were estimated ≥ 2000 mg / kg body weight in acute toxicity, present sub-acute toxicity in rodent animal models.…”