Evaluation of Antimicrobial Efficacy of various Intracanal Medicaments in Primary Teeth: Anin vivoStudy

Abstract

Introduction Bacteria and their products play a primary etiological role in the initiation and perpetuation of pulpoperiapical pathosis. Intracanal medication is important for endodontic success as it eliminates microorganisms that persist after chemomechanical preparation.Aim To compare antimicrobial efficacy of calcium hydroxide powder, triple antibiotic paste, calcium hydroxide with 2% chlorhexidine solution, and triple antibiotic paste with 2% chlorhexidine solution.Materials and methods A total of 48 nonvital primary teeth were included in this study. After access opening first microbiological sample (s1) was collected by using absorbent paper point introducing into canal. Second microbilogical sample (s2) was taken following chemomechanical preparation and the teeth were divided into four groups: Group I: calcium hydroxide (CH) powder with distilled water; group II: CH with 2% chlorhexidine solution; group III: triple antibiotic powder with distilled water; group IV: triple antibiotic paste with 2% chlorhexidine solution. Then the canals were filled with any one group of the medicament and cavity was temporarily sealed with zinc oxide eugenol. After 1 week, a postmedication sample (s3) was collected. Then the canal was filled with Metapex, restored with glass ionomer cement.Conclusion From the experiments carried out in this study, with the limitations, an inference can be drawn that a combination of antimicrobial agent used as intracanal medicament is definitely better than single agent like Ca(OH)2.How to cite this article Dutta B, Dhull KS, Das D, Samir PV, Verma RK, Singh N. Evaluation of Antimicrobial Efficacy of various Intracanal Medicaments in Primary Teeth: An in vivo Study. Int J Clin Pediatr Dent 2017;10(3):267-271.

Antimicrobial Efficacy of Intracanal Medicaments against E. Faecalis Bacteria in Infected Primary Molars by Using Real-Time PCR: A Randomized Clinical Trial

“…Clinical evaluations of the antimicrobial efficacy of intracanal medicaments are usually performed on E . faecalis bacteria, which is among the most resistant and prevalent microorganisms found in the root canal system with practical access to the genomic consequence and primer design [ 8 , 12 , 13 ]. Calcium hydroxide is widely used as an intracanal agent, known as the gold standard endodontic medicament that eliminates microbes that survive instrumentation.…”

“…faecalis [ 26 , 29 ]. Dutta et al [ 8 ] evaluated the efficacy of combination of medicaments (TAP, CH, and chlorhexidine) in comparison with CH per se against E. faecalis in necrotic primary teeth in clinical conditions, by culturing and counting colony-forming units. They found that a combination of antimicrobial agents used as intracanal medicament was definitely better than a single agent such as Ca (OH) 2 .…”

“…Calcium hydroxide (CH) is commonly used in endodontics as an intracanal medicament between the treatment sessions for necrotic permanent teeth [ 8 , 9 ]. However, it does not affect some bacterial strains such as E. faecalis [ 9 – 11 ].…”

“…However, it does not affect some bacterial strains such as E. faecalis [ 9 – 11 ]. Infections in the primary root canals are associated with a broad variety of microorganisms, among which E. faecalis is one of the most prevalent and resistant bacteria species in both deciduous and permanent root canals [ 8 , 12 – 14 ].…”

“…The need to wipe out deeply penetrated resistant bacteria from the dentinal tubules and improve the treatment of infected teeth have resulted in introduction of other medicaments such as triple antibiotic paste [ 7 ], which has been successfully used in primary and permanent necrotic canals [ 15 , 16 ]. Application of triple antibiotic paste (TAP) as an intracanal medicament was observed to completely sterilize the infected canals within two weeks, proving to be certainly better than a single agent such as CH [ 7 , 8 , 17 ]. Higher success rate was reported in primary teeth treated with TAP compared with conventional pulpectomy [ 7 ].…”