Evaluation of Bacillus subtilis Effect as Probiotic on Hematological Parameters of Rainbow Trout, Oncorhynchus mykiss (Walbaum) Following Experimental Infection with Streptococcus iniae

Kamgar, Maryam; Ghane, Masood

doi:10.3923/jfas.2012.422.430

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…Different probiotics are often reported to stimulate piscine immune system (Nayak 2010). Dietary supplementation of B. subtilis often improves the growth and nutrition, immunohaematological responses, and disease resistance in different fish species (Nayak et al 2007;Aly et al 2008;Kamgar and Ghane 2012;Liu et al 2012;Purwandari and Chen 2013), and the findings of the present study corroborated to these earlier studies. Here in this study, apart from different haematological parameters B. subtilis treatment was also found to affect various immunological parameters of catla during pre-and post-challenge.…”

Section: Discussionsupporting

confidence: 89%

“…In a previous study, it was also reported that supplementation with B. subtilis could increase TLC in L. rohita (Kumar et al 2006). Recently, Kamgar and Ghane (2012) also recorded significant increase in leucocyte count in rainbow trout, Oncorhynchus mykiss fed with B. subtilis when challenged with Streptococcus iniae. The result from present experiment also revealed an increase in TLC in treatment groups (T 1 , T 2 , T 3 , and T 4 ) compared with both the control (C p and C n ) groups.…”

Section: Discussionmentioning

confidence: 82%

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Effect of dietary supplementation of Bacillus subtilis on haematological and immunological parameters of Catla catla (Hamilton)

et al. 2015

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Effect of dietary supplementation of a Gram-positive, aerobic, probiotic bacterium Bacillus subtilis on the immunohaematological indices during pre-and post-challenge in Indian major carp, catla (Catla catla), was studied. The B. subtilis was administered orally at four different doses 1.0 9 10 6 (T 1 ), 1.0 9 10 7 (T 2 ), 1.0 9 10 8 (T 3 ), and 1.0 9 10 9 (T 4 ) cfu g -1 feed to C. catla for 90 days. The positive control (C p ) and negative control (C n ) were fed with feed without B. subtilis for the same period. On the 60th day, blood and serum were sampled to determine various haematological and serum parameters. Fish were challenged intraperitoneally with Aeromonas hydrophila after 60 days in all the treatment groups and C p , while the C n was challenged with phosphatebuffered saline (PBS, pH 7.2) only. Dietary supplementation of B. subtilis leads to the rise of various immunological and haematological parameters in catla during the pre-and postchallenge. During pre-challenge, the highest TEC (1.30 ± 0.02 9 10 6 cells mm -3 ), haemoglobin (7.43 ± 0.25 g %), total serum protein (3.89 ± 0.08 g dL -1 ), and serum lysozyme activity (8.39 ± 0.01 lg ml -1 ) were recorded in fishes fed feed containing B. subtilis at 1 9 10 9 cfu/g feed (T 4 ). The highest survival percentage (86.33 %) was also observed in T 4 group. The significantly increased survival percentage (P \ 0.05) of B. subtilis-treated groups in comparison with control group (C p ) suggests that dietary supplementation of this probiotic bacterium can protect catla from A. hydrophila infection by enhancing innate immunity.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 82%

Effect of dietary supplementation of Bacillus subtilis on haematological and immunological parameters of Catla catla (Hamilton)

et al. 2015

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“…2006) and E. tarda (Nayak et al . 2007); and rainbow trout, O. mykiss against Streptococcus iniae (Kamgar & Ghane 2012). On the contrary, researchers have also failed to demonstrate the protective action of this bacterium against pathogens although stimulated their immunity.…”

Section: Applications Of Bacillus Subtilis In Aquaculturementioning

confidence: 99%

Multifaceted applications of probiotic Bacillus species in aquaculture with special reference to Bacillus subtilis

Nayak

2020

Reviews in Aquaculture

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Microorganisms play an important role in water quality maintenance, waste management, productivity enhancement, nutrient cycling, functional feed development, protection against infectious diseases, delivery of antigens and several other health benefits in aquaculture. The most significant use of microbes in aquaculture to minimize the antibiotics and drugs is through probiotics. The scope of probiotics in modern aquaculture has been enormous due to the detrimental consequences of routinely used antibiotics and chemotherapeutic agents and demand for safe aquatic products for human consumption. Since past few decades, both scientific and commercial interests are burgeoning in this field, and over the years, several probiotics are successfully used in aquaculture. Among various probiotics that are being used, Bacillus species are the common probiotics that are used in different aquatic animals. Several probiotics species belonging to Bacillus genus are currently been used, and Bacillus subtilis is one of such probiotics that is very well‐researched and most widely used probiotics in aquaculture. While its probiotic use has already been an important component in diverse aquaculture practices, its application in water quality improvement, utility in bioflocs/bioremediation and vehicle to express and delivery of heterologous antigen are noteworthy. It exerts innumerable beneficial effects such as improvement of growth, nutrition, immunity and disease resistance in aquatic species. This bacterium adopts a variety of disparate and overlapping mechanisms such as synergistic, antagonistic, competitive exclusion and immune‐stimulating effects. This review summarizes various applications of this bacterium as probiotics, bioremediating agent, bioflocing agent and possible live vaccine vehicle in aquaculture.

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“…Such mechanisms form a series of essential functions that keep host cells alive, healthy, and protected from pathogens. The use of Bacillus species has been shown to elicit inhibitory responses to numerous fish diseases, such as red mouth disease, edwardsiellosis, furunculosis, lactococcusis, streptococcusis, vibriosis and aeromoniasis (Harikrishnan et al, 2010;Brunt et al, 2007;Ridha et al, 2003;Kumar et al, 2006;Taoka et al, 2006;Kamgar et al, 2012 ). ALT and AST, two liver enzymes, decreased in the probiotic diet fish group compared to the control (Fig.…”

Section: Discussionmentioning

confidence: 90%

Dietary Effect of Probiotic Bacteria, Bacillus amyloliquefaciens-JFP2 on Growth and Innate Immune Response in Rock Bream Oplegnathus fasciatus, Challenged with Streptococcus iniae

Heo¹,

Dharaneedharan²,

Kim³

2017

Israeli Journal of Aquaculture - Bamidgeh

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The present study was conducted to evaluate the use of Bacillus amyloliquefaciens as a probiotic source in the diet of rock bream, Oplegnathus fasciatus. A total of 210 fingerlings (25.4 ± 0.13 g) were divided into two groups, three replicates each; control (C) and probiotic (P) group (35×3=105 fish per group). C group was fed a basal diet without probiotic while P group was fed with B. amyloliquefaciens spores at a concentration of 1.4 ×10 6 colony-forming units per gram (CFU/g) of feed. After 90 days, P group fish showed significant improvements in body weight (BW), weight gain (WG), specific growth rate (SGR) and food conversion ratio (FCR) when compared with C group fish. Also, in biweekly assessments, serum protein and glucose level showed a significant increase (p<0.05) in probiotic fed fish compared with the control group. Similar increase in serum antioxidant and lysozyme activity was found in the probiotic fed fish group. However, there was a significant decrease in triglyceride and total cholesterol along with increased alanine aminotransferase (ALT) and aspartate amonotransferase (AST) levels in probiotic diet fed fish compared to the control. After seventy days the fish were challenged with Streptococcus iniae and mortality of the probiotic fed fish was significantly lower (p<0.05) when compared with the control. These results demonstrate the benefit of incorporation of B. amyloliquefaciens as a feed supplement to improve the health status of Oplegnathus fasciatus challenged with Streptococcus iniae. The IJA appears exclusively as a peer-reviewed on-line open-access journal at http://www.siamb.org.il. To read papers free of charge, please register online at registration form. Sale of IJA papers is strictly forbidden.

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Evaluation of Bacillus subtilis Effect as Probiotic on Hematological Parameters of Rainbow Trout, Oncorhynchus mykiss (Walbaum) Following Experimental Infection with Streptococcus iniae

Cited by 13 publications

References 29 publications

Effect of dietary supplementation of Bacillus subtilis on haematological and immunological parameters of Catla catla (Hamilton)

Effect of dietary supplementation of Bacillus subtilis on haematological and immunological parameters of Catla catla (Hamilton)

Multifaceted applications of probiotic Bacillus species in aquaculture with special reference to Bacillus subtilis

Dietary Effect of Probiotic Bacteria, Bacillus amyloliquefaciens-JFP2 on Growth and Innate Immune Response in Rock Bream Oplegnathus fasciatus, Challenged with Streptococcus iniae

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