Evaluation of basic fibroblast growth factor in tympanic membrane repair

Abstract

Basic fibroblast growth factor (bFGF) has been shown to enhance speed of wound repair at a number of anatomic sites. This study presents an evaluation of bFGF in a model of acute tympanic membrane (TM) perforation in order to assess rate of healing as well as structural and functional outcome. Perforations were created in the tympanic membranes of rats, then allowed to heal in the presence of topically applied bFGF with the opposite ear serving as a control. The growth factor was applied in repeated doses beginning 2 days after creation of the TM defect. The treated ears healed faster by an average of 4.0 days. The healed tympanic membranes were assessed using tympanometry and light microscopy. Structurally and functionally, the healed tympanic membranes were similar to the controls. The observed results indicate that bFGF promotes accelerated healing and restoration of normal architecture in acute TM defects.

Miringoplastia com a utilização de um novo material biossintético

“…Estudos realizados nas duas últimas décadas apontam a importância de fatores de crescimento próprios da membrana timpânica normal e a expressão destes fatores ocorrendo de forma diferente em membranas timpânicas perfuradas [23][24][25][26][27][28][29][30][31][32][33][34][35] .…”

“…A aquisição desses fatores de crescimento é possível por técnica de DNA recombinante que é complexa [21][22][23][24][25][26][27][28][29][30][31][32] .…”

Expression of Fibroblast Growth Factor Receptors 1–4 in Human Chronic Tympanic Membrane Perforation

“…Such therapeutic effects of bFGF are also seen when it is applied exogenously. Furthermore, bFGF effectively increases the healing of TM perforations in guinea pigs [10][11][12] , rats [13][14][15] , and chinchillas [16] . These clinical and experimental results suggest that bFGF affects the healing of TM perforation.…”

Keratinocyte growth factor 1, fibroblast growth factor 2 and 10 in the healing tympanic membrane following perforation in rats

“…Orientation of the fibroblast is already underway (Mondain et al 1991). Another study showed treated TMs healed on average 4 days earlier than control TMs (Vrabec et al 1994). Further studies showed no significant difference in healing time (Chauvin et al 1999;Friedman et al 1997).…”

“…There are several studies examining the role of FGF2 application to the acutely healing tympanic membrane (TM) (Mondain and Ryan 1994;Fina et al 1991;Fina et al 1993;Mondain et al 1991;Vrabec et al 1994). Research suggests that only high doses have a significant effect but these effects were counterbalanced by the effects of a hypertrophic wound, possibly due to the gelfoam TM used for application (Mondain et al 1991).…”