Ruta chalepensis L is commonly known as rue, is used in the traditional medicine of many countries for the treatment of various diseases.Recent pharmacological activities like antioxidant, anticancer, and anti-inflammatory effects are reported in this species. The aim of the study is to evaluate the biological constituents of Ruta chalepensis L(MK828113) plant extracts. The phytochemical screening tests were conducted as per the standard procedure to identify the compounds present in the leaf extracts of Ruta chalepensis. The leaves of this plant revealed the presence of alkaloids, flavonoids, phenols, quanines, and steroids with different bioactive compounds. The antibacterial activities of these extracts were examined using E.coli, B.subtilis, S.aureus, and P.aeruginosa to find the microbial inhibiting activities of the bioactive compounds. The bioactive compounds of these plants are great, interest in medicinal chemistry. The antioxidant activity such as DPPH, FRAP, and SOD was carried out and evaluated according to the dose-dependent manner. The compounds were further characterized using TLC and GC-MS analysis.