Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Ribeiro, Jorge; Pereira, Tiago; Caseiro, Ana Rita; Armada-da-Silva, Paulo; Pires, Isabel; Prada, Justina; Amorim, Irina; Amado, Sandra; França, Miguel Lacueva; Gonçalves, Cláudia; Lopes, M. A.; Santos, J. D.; Silva, Dina Morais; Geuna, Stefano; Luís, Ana Lúcia; Maurício, Ana Colette

doi:10.4252/wjsc.v7.i6.956

Cited by 21 publications

(40 citation statements)

References 65 publications

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“…More importantly, neuronal cell differentiation from mES cells on the MWCNT-coated hydrogel scaffolds was observed, forming extensive nerve networks around each fiber. In addition, Mauricio's research team has successfully used tube guides of polyvinyl alcohol (PVA) loaded with functionalized MWCNTs for in vivo peripheral nerve regeneration [19][20][21][22][23]. In one study, they investigated tubular PVA-MWCNT guides for neuromuscular regeneration after an axonotmesis injury in rat sciatic nerves [22].…”

Section: Introductionmentioning

confidence: 99%

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

et al. 2018

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Objective. Nanomaterials, such as carbon nanotubes (CNTs), have been introduced to modify the surface properties of scaffolds, thus enhancing the interaction between the neural cells and biomaterials. In addition to superior electrical conductivity, CNTs can provide nanoscale structures similar to those present in the natural neural environment. The primary objective of this study is to investigate the proliferative capability and differential potential of neural stem cells (NSCs) seeded on a CNT incorporated scaffold. Approach. Amine functionalized multi-walled carbon nanotubes (MWCNTs) were incorporated with a PEGDA polymer to provide enhanced electrical properties as well as nanofeatures on the surface of the scaffold. A stereolithography 3D printer was employed to fabricate a well-dispersed MWCNT-hydrogel composite neural scaffold with a tunable porous structure. 3D printing allows easy fabrication of complex 3D scaffolds with extremely intricate microarchitectures and controlled porosity. Main results. Our results showed that MWCNT-incorporated scaffolds promoted neural stem cell proliferation and early neuronal differentiation when compared to those scaffolds without the MWCNTs. Furthermore, biphasic pulse stimulation with 500 µA current promoted neuronal maturity quantified through protein expression analysis by quantitative polymerase chain reaction. Significance. Results of this study demonstrated that an electroconductive MWCNT scaffold, coupled with electrical stimulation, may have a synergistic effect on promoting neurite outgrowth for therapeutic application in nerve regeneration.

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Section: Introductionmentioning

confidence: 99%

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

et al. 2018

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“…Briefly, applying a casting technique using a silicone mould, a PVA aqueous solution 15% (w/v), a PVA aqueous solution 15% (w/v) supplemented with 0.05% of COOH‐functionalized MWCNTs and a PVA aqueous solution 15% (w/v) supplemented with a 0.05% of PPy were prepared. The membranes were produced by freezing/thawing cycles as described in Ribeiro et al and Gonçalves et al . Gamma‐radiation was used as sterilization method and the membranes were hydrated in a sterile saline solution during 2 h before microsurgical application in the rat axonotmesis injuries (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…). Membranes physical ‐ chemical characterization included electrical conductivity assessment, Differential Scanning Calorimetry (DSC) and enthalpy determination (ΔH), Fourrier Transform Infrared Spectroscopy (FTIR), X‐Ray Diffraction (XRD), Scanning Electronic Microscopy (SEM), and wettability determination, and the results were published elsewhere …”

Section: Methodsmentioning

confidence: 99%

“…Many studies have demonstrated that the chemical composition of the local nerve injury environment is crucial for nerve regeneration including the presence of growth factors . The association of biomaterials that not only have a positive effect on peripheral nerve regeneration but are also capable of carrying or supporting a cellular system responsible for orchestrating this environment, has been recently demonstrated by our research group . The morphological evaluation of the muscle tibialis anterior (TA) regeneration after the treatment of an axonotmesis injury with these three different membranes permitted the evaluation of neuromuscular recovery (and the neurogenic atrophy), and to correlate with the functional and morphological recoveries of the injured sciatic nerves .…”

Section: Introductionmentioning

confidence: 99%

“…When full peripheral nerve transection injuries (neurotmesis) occur, reconstructive surgical procedures are always necessary but in case of axonotmesis injuries the surgical procedures are not necessary although the use of biomaterials or cellular therapies may increase the regeneration rate and diminish the neurogenic muscular atrophy responsible for functional disabilities . However, this injury model is often used in pre‐clinical validations of biomaterials or cell‐based therapies as it is a very well‐studied model . The use of electrically conductive biomaterials to produce tube‐guides is a promising therapeutic, especially when the neurotmesis injuries have a gap which invalidates an end‐to‐end suture.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of PVA biodegradable electric conductive membranes for nerve regeneration in axonotmesis injuries: the rat sciatic nerve animal model

Ribeiro

Caseiro

Pereira

et al. 2017

J Biomedical Materials Res

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The therapeutic effect of three polyvinyl alcohol (PVA) membranes loaded with electrically conductive materials - carbon nanotubes (PVA-CNTs) and polypyrrole (PVA-PPy) - were tested in vivo for neuro-muscular regeneration after an axonotmesis injury in the rat sciatic nerve. The membranes electrical conductivity measured was 1.5 ± 0.5 × 10 S/m, 579 ± 0.6 × 10 S/m, and 1837.5 ± 0.7 × 10 S/m, respectively. At week-12, a residual motor and nociceptive deficit were present in all treated groups, but at week-12, a better recovery to normal gait pattern of the PVA-CNTs and PVA-PPy treated groups was observed. Morphometrical analysis demonstrated that PVA-CNTs group presented higher myelin thickness and lower g-ratio. The tibialis anterior muscle, in the PVA-PPy and PVA-CNTs groups showed a 9% and 19% increase of average fiber size area and a 5% and 10% increase of the "minimal Feret's diameter," respectively. No inflammation, degeneration, fibrosis or necrosis were detected in lung, liver, kidneys, spleen, and regional lymph nodes and absence of carbon deposits was confirmed with Von Kossa and Masson-Fontana stains. In conclusion, the membranes of PVA-CNTs and PVA-PPy are biocompatible and have electrical conductivity. The higher electrical conductivity measured in PVA-CNTs membrane might be responsible for the positive results on maturation of myelinated fibers. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1267-1280, 2017.

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Peripheral Nerve Regeneration: Current Status and New Strategies Using Polymeric Materials

Pinho

Fonseca

Serra

et al. 2016

Adv Healthcare Materials

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Experiments concerning peripheral nerve regeneration have been reported since the end of the 19th century. The need to implement an effective surgical procedure in terms of functional recovery has resulted in the appearance of several approaches to solve this problem. Nerve autograft was the first approach studied and is still considered the gold standard. Since autografts require donor harvesting, other strategies involving the use of natural materials have also been studied. Nevertheless, the results were not very encouraging and attention has moved towards the use of nerve conduits made from polymers, whose properties can be easily tailored and which allow the nerve conduit to be easily processed into a variety of shapes and forms. Some of these materials are already approved by the US Food and Drug Administration (FDA), as is presented here. Furthermore, polymers with conductive properties have very recently been subject to intensive study in this field, since it is believed that such properties have a positive influence in the regeneration of the new axons. This manuscript intends to give a global view of the mechanisms involved in peripheral nerve regeneration and the main strategies used to recover motor and sensorial function of injured nerves.

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Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Cited by 21 publications

References 65 publications

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

Evaluation of PVA biodegradable electric conductive membranes for nerve regeneration in axonotmesis injuries: the rat sciatic nerve animal model

Peripheral Nerve Regeneration: Current Status and New Strategies Using Polymeric Materials

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